Table 3.

Changes in mPSC parameters in GnRH neurons on E2 administration

Frequency of mPSCsFrequency of control period (Hz)Percentage change (%)N/ndftp
Pro pm E21.869 ± 0.1877153.2 ± 17.3312/7113.0670.0107a
Pro am E21.074 ± 0.2078110.2 ± 8.5812/8111.1890.2593a
Met pm E21.120 ± 0.229080.6 ± 7.549/682.5910.0321a
Pro pm PHTPP+E22.059 ± 0.228193.4 ± 9.077/460.72430.4962a
Pro pm intraPHTPP+E21.790 ± 0.512997.1 ± 9.167/560.32220.7582a
Pro pm CPTIO+E22.006 ± 0.2569106.1 ± 5.939/581.0270.3346a
Pro pm PP2+E21.555 ± 0.2891106.2 ± 6.068/671.0140.3441a
Pro pm LY294002+E21.703 ± 0.1989102.2 ± 5.888/670.38050.7148a
Pro pm Kynu+E21.279 ± 0.2357133.7 ± 12.277/462.7460.0335a
Pro pm Picro+E20.631 ± 0.1376143.1 ± 17.237/462.4920.0470a
Amplitude of mPSCsAmplitude of control period (pA)Percentage change (%)Ndftp
Pro pm E2-24.43 ± 2.067100.7 ± 2.1912/7110.27600.7952a
Pro am E2-36.41 ± 5.148101.5 ± 1.1512/8111.3430.2062a
Met pm E2-34.51 ± 6.257101.2 ± 2.849/680.43010.6785a
Pro pm PHTPP+E2-26.48 ± 3.56297.0 ± 2.197/461.3680.2203a
Pro pm intraPHTPP+E2-27.72 ± 8.61499.1 ± 1.187/560.73370.4908a
Pro pm CPTIO+E2-25.05 ± 2.062102.7 ± 2.1929/581.2160.2585a
Pro pm PP2+E2-26.46 ± 2.464101.8 ± 3.848/670.46380.6569a
Pro pm LY294002+E2-36.90 ± 4.897100.7 ± 1.188/670.58120.5793a
Pro pm Kynu+E2-36.83 ± 2.28698.1 ± 2.377/460.77220.4693a
Pro pm Picro+E2-19.64 ± 2.10797.0 ± 2.587/461.1620.2894a
Rise τ of mPSCsRise τ of control period (ms)Percentage change (%)Ndftp
Pro pm E23.941 ± 0.5402138.8 ± 43.1312/7110.89850.3882a
Pro am E24.287 ± 0.1746134.3 ± 20.2912/8111.6880.1194a
Met pm E24.760 ± 0.6285109.1 ± 16.419/680.55520.5939a
Pro pm PHTPP+E23.710 ± 0.2690103.3 ± 14.277/460.23020.8256a
Pro pm intraPHTPP+E24.175 ± 0.9160116.7 ± 24.647/560.67630.5241a
Pro pm CPTIO+E23.771 ± 0.4979143.9 ± 44.249/581.6110.1459a
Pro pm PP2+E23.970 ± 0.446699.1 ± 13.228/670.06570.9494a
Pro pm LY294002+E23.708 ± 0.287092.6 ± 9.098/670.8180.4403a
Pro pm Kynu+E24.220 ± 0.4710101.2 ± 14.997/460.07890.9396a
Pro pm Picro+E23.006 ± 0.1134124.4 ± 27.997/460.87290.4163a
Decay τ of mPSCsDecay τ of control period (ms)Percentage change (%)Ndftp
Pro pm E210.60 ± 2.416137.2 ± 27.4412/7111.6560.1259a
Pro am E213.04 ± 5.393130.8 ± 24.2012/8111.2720.2297a
Met pm E213.34 ± 1.805141.4 ± 23.439/681.7680.1150a
Pro pm PHTPP+E213.44 ± 3.977105.3 ± 22.417/460.23590.8214a
Pro pm intraPHTPP+E211.83 ± 1.433125.7 ± 16.457/561.5620.1692a
Pro pm CPTIO+E213.62 ± 2.368113.4 ± 22.799/580.58980.5716a
Pro pm PP2+E210.67 ± 1.03394.5 ± 7.388/670.74550.4803a
Pro pm LY294002+E213.45 ± 2.55997.3 ± 9.518/670.28530.7837a
Pro pm Kynu+E212.48 ± 1.152103.5 ± 8.277/460.42690.6843a
Pro pm Picro+E25.62 ± 1.10179.3 ± 21.577/460.96020.3740a
  • N = number of neurons measured. n = number of animals used for the given experiment.

  • df = degree of freedom of Student’s t test of percentage data where each neuron serves as its own control.

  • t = t values of Student’s t test of percentage data.

  • p = p probability values of Student’s t test of percentage data.

  • Pro = proestrus; Met = metestrus; am = morning; pm = afternoon.