Figures | Experiment | n(N) | Test used | p value | Mean (CI)* |
---|---|---|---|---|---|
1A | In vitro, interface, P55 | WT: 10(3), KO: 9(3) | ANOVA | 0.0025 | see 1Bleft |
1B | Left: in vitro, interface, P55 | WT: 10(3), KO: 9(3) | Two-way ANOVA | Experimental condition: 0.89 genotype: <0.0001 | WT: 38.3 (18.2), KO: 76.4 (12.0) |
Right: in vitro, submerged, P25 | WT: 30(5), KO: 34(6) | WT: 37.6 (6.1), KO: 56.3 (6.9) | |||
1C | Left: in vitro, interface, P25 | WT: 4(2), KO: 4(2) | Two-way ANOVA | Experimental condition: 0.98 genotype: 0.0005 | WT: 1.00 (0.31), KO: 0.67 (0.29) |
Middle: in vitro, submerged, P25 | WT: 8(3), KO: 11(4) | WT: 1.00 (0.24), KO: 0.71 (0.14) | |||
Right: in vitro, interface, P85 | WT: 9(3), KO: 9(3) | WT: 1.00 (0.20), KO: 0.69 (0.12) | |||
1D* | Left: in vitro, submerged, P25 | WT: 18(8), KO: 19(6) | Mann–Whitney U | 0.013 | WT: 1.0 [0.8 1.4], KO: 0.7 [0.6 0.9] |
Middle: in vitro, submerged, P25 | WT: 20(6), KO: 18(8) | Mann–Whitney U | 0.036 | WT: 1.0 [0.6 1.2], KO: 0.5 [0.4 1.1] | |
Right: in vitro, interface, P25 | WT: 12(2), KO: 12(2) | Mann–Whitney U | 0.29 | WT: 1.0 [0.5 1.3], KO: 1.2 [0.7 1.7] | |
2A | In vivo, P50, 3h-LTP | WT: 16, KO: 15 | For LTP induction: two-way ANOVA | LTP (categorical): WT: 0.017, KO: <0.0001 time (continuous): WT: 0.42, KO: 0.005 | LTP 2-3h postinduction (%): WT: 9.1 (8.6), KO: 32.6 (25.5) |
Between genotypes: two-way ANOVA | genotype: <0.0001 time: 0.016 | ||||
2B | In vivo, P50, 2h-LTP | WT: 16, KO: 16 | For LTP induction: two-way ANOVA | LTP (categorical): WT: <0.0001, KO: 0.002 time (continuous): WT: 0.02, KO: 0.15 | LTP 1-2h postinduction (%): WT: 15.0 (6.9), KO: 15.0 (12.1) |
Between genotypes: two-way ANOVA | Genotype: 0.82 time: 0.048 | ||||
2C | In vivo, P50 | WT: 12, KO: 8 | Two-way ANOVA | Genotype: 0.011 stimulation: <0.0001 | WT125μA: 1.9 (1.1), KO125μA: 1.1 (0.5) |
3A | In vivo, P50, L-LTP | WT: 10, KO: 10 | For LTP induction: two-way ANOVA | LTP (categorical): WT: <0.0001, KO: 0.012 time (continuous): WT: 0.007, KO: 0.74 | LTP 2-3h postinduction (%): WT: 12.9 (11.2), KO: 14.3 (12.0) |
Between genotypes: two-way ANOVA | Genotype: 0.79 time: 0.17 | ||||
3B | In vivo, P50, E-LTP | WT: 10, KO: 10 | For LTP induction: two-way ANOVA | LTP (categorical): WT: 0.0002, KO: <0.0001 time (continuous): WT: 0.022, KO: 0.003 | LTP 1-2h postinduction (%): WT: 12.0 (18.6), KO: 10.6 (13.2) |
Between genotypes: two-way ANOVA | Genotype: 0.16 time: 0.006 | ||||
3C | In vivo, P50 | WT: 5, KO: 5 | Two-way ANOVA | Genotype: 0.94 stimulation: <0.0001 | WT125μA: 2.2 (1.9), KO125μA: 1.7 (1.4) |
4A | In vitro, submerged, P25 | WT: 11(6) | Student’s t test (paired) | WT: 0.19 | WT−60mV: 16.3 (6.6), WT+40mV: 13.2 (5.6) |
KO: 14(6) | Student’s t test (paired) | KO: 0.0003 | KO−60mV: 36.3 (10.9), KO+40mV: 12.7 (4.1) | ||
4C | In vitro, submerged, P25 | WT: 11(6), KO: 13(6) | Student’s t test | 0.0018 | WT: 3.1 (4.4), KO: 23.6 (9.4) |
4D* | In vitro, submerged, P25 | WT: 20(6), KO: 18(8) | Mann–Whitney U | 0.036 | WT: 1.9 [1.1 2.3], KO: 0.9 [0.7 2.0] |
4E | In vitro, submerged, AMPA/NMDA ratio* | P13–P14: WT: 16(4) KO: 18(5) P21–P24: WT: 10(3), KO: 11(3) P25–P28: WT: 10(4), KO: 7(3) | Over age groups: Kruskal–Wallis test | WT: 0.0026, KO: 0.056 | P13–P14: WT: 0.68 [0.47 0.88], KO: 0.45 [0.31 0.64] P21–P24: WT: 0.75 [0.55 1.09], KO: 0.63 [0.43 0.93] P25–P28: WT: 1.20 [0.88 1.48], KO: 0.78 [0.50 1.00] |
Between genotypes: Mann–Whitney U | P13–P14: 0.055 P21–P24: 0.083 P25–P28: 0.016 | ||||
In vitro, submerged, LTP | P13–P14: WT: 10(2), KO: 7(2) P21–P24: WT: 15(2), KO: 14(2) P25–P28: WT: 15(3), KO: 20(3) | Over age groups: ANOVA | WT: 0.012, KO: 0.77 | P13–P14: WT: 51.6 (12.9), KO: 61.6 (11.4) P21–P24: WT: 44.6 (7.9), KO: 58.2 (13.2) P25–P28: WT: 30.5 (8.2), KO: 55.3 (7.8) | |
Between genotypes: Student’s t test | P13–P14: 0.30 P21–P24: 0.09 P25–P28: 0.0002 |
↵* Median and percentiles [25th 75th] are reported for nonparametric datasets instead of mean and CI values.