Table 3.

Summary of statistical analyses

LocationDescriptionData structureType of testStatistical value
aFig. 1BENormal (Gaussian distribution) at the end point; number (n) of biological experiments = 4 with three technical replicatesTwo-way repeated-measures ANOVA after Bonferroni correctionNumber (#) of MNs : p < 0.001, F = 13.63, DFn = 1; Neurite length per cell: p < 0.01, F = 5.27, DFn = 1; # of Nodes per cell : p < 0.001, F= 30.57, DFn = 1; Cell body size per cell : p < 0.001, F = 11.54, DFn = 1
Unpaired two-tailed t-test at the end point# of MNs : p < 0.001; Neurite length per cell: p < 0.01; # of Nodes per cell : p < 0.001; Cell body size per cell : p < 0.001
bFig. 1FNormal, n = 4Unpaired two-tailed t-testp < 0.05
cFig. 1GNormal, n = 4Unpaired two-tailed t-test# of MNs: p < 0.01, Neurite length per cell: p < 0.05; # of Nodes per cell: p < 0.0001, Cell body size: p < 0.01
d Fig. 2CNormal, n = 5Unpaired two-tailed t-testTF+: p < 0.001, TF addback at day 6: p < 0.05
e Fig. 2D, ENormal at the end point; n = 5Two-way repeated-measures ANOVA after Bonferroni correctionNeurite length per cell: p < 0.01, F = 6.555, DFn = 4; # of Nodes per cell: p < 0.05, F = 3.356, DFn = 4
Unpaired two-tailed t-test at the end pointNeurite length per cell: TF addback at day 6, day 7, and day 8: p < 0.001, respectively; # of Nodes per cell: TF addback at day 6, day 7: p < 0.001; TF addback at day 8: p < 0.01
f Fig. 2GNormal, n = 5Unpaired two-tailed t-testTF+ and TF+/Ken 5µM: p < 0.001; TF/2.5µM and TF/5µM: p < 0.05
g Fig. 2H, INormal at the end point; n = 5Two-way repeated-measures ANOVA after Bonferroni correctionNeurite length per cell: p < 0.05, F = 4.01, DFn = 4; # of Nodes per cell: not significant
Unpaired two-tailed t-test at the end pointNeurite length per cell: TF+ and TF+/Ken 5µM: p < 0.001; # of Nodes per cell: TF+: p < 0.001,; TF+/Ken5 µM: p < 0.01
h Fig. 3DNormal at the end point; n = 5Two-way repeated-measures ANOVA correction after Bonferroni correctionFold change of MNs with 4 or more nodes :p < 0.05, F = 3.949, DFn = 2
Unpaired two-tailed t-test at the end pointp < 0.05
i Fig. 4CNormal, n = 5Unpaired two-tailed t-testTF+: p < 0.001, TF addback at day 6: p < 0.001
j Fig. 4DNormal, n = 5Unpaired two-tailed t-testTF+: p < 0.01, TF+/Ken 5 µM: p < 0.01
k Fig. 5CNormal, n = 5Unpaired two-tailed t-testTF+: p < 0.001, TF addback at day 6: p < 0.001, TF addback at day 7: p < 0.05, addback at day 8: p < 0.05,
l Fig. 5DNormal, n = 5Unpaired two-tailed t-testTF+: p < 0.001, TF addback at day6: p < 0.001,; TF addback at day7: p < 0.05, addback at day8: p < 0.05
m Fig. 5ENormal, n = 5Unpaired two-tailed t-testTF+: p < 0.001, TF+/Ken 5 µM: p < 0.001,; TF/Ken 2.5 µM: p < 0.01, TF/Ken 5µM: p < 0.01
n Fig. 5FNormal, n = 5Unpaired two-tailed t-testTF+: p < 0.01, TF+/Ken 5 µM: p < 0.01
o Fig. 6CNormal, n = 5Unpaired two-tailed t-testTF addback at day 6, day 7, and day 8: p < 0.001, respectively
p Fig. 6DNormal, n = 5Unpaired two-tailed t-testTF addback at day 6, day 7, and day 8: p < 0.001, respectively