Table 1.

Antibodies used for immunoblotting

Primary antibodyMade inSupplierPart numberDilutionSecondary antibody (dilution)*
Anti-mGluR1RbMillipore07-6171:500Dk anti-Rb IRDye 700 (1:20,000)
Anti-mGluR5MsMilliporeMABN5401:500Dk anti-Ms IRDye 800 (1:15,000)
Anti-mGluR2/3RbMillipore06-6761:1000Dk anti-Rb IRDye 700 (1:20,000)
Anti-mGluR4RbMilliporeAB150971:1000Dk anti-Rb IRDye 800 (1:15,000)
Anti-mGluR7GtAbcamab853431:1000Dk anti-Gt IRDye 700 (1:20,000)
Anti-mGluR8GtSanta Cruz Biotechnologysc-303001:500Dk anti-Gt IRDye 800 (1:15,000)
Anti-α-tubulinCkSigma-AldrichSAB35000231:2000Dk anti-Ck IRDye 700 (1:20,000)
  • Ck = chicken, Dk = donkey, Gt = goat, Ms = mouse, Rb = rabbit.

  • * All secondary antibodies were purchased from LI-COR Bioscience.