Table 1.

Statistical summary

ItemFigureData structureType of testDescriptionComparisonPower
a1FNormal distributionMantel–Cox log rank test; Bonferroni post hoc analysis; K = 6Survival postinjection
Control vs. P13 ablation DKOχ2 = 51.18; p < 0.0001
P13 ablation DKO vs. P23 ablation DKOχ2 = 25.28; p < 0.0001
P13 ablation DKO vs. P93 ablation DKOχ2 = 17.72; p < 0.0001
Control vs. P23 ablation DKOx2 = 31.28; p < 0.0001
P23 ablation vs. P93 ablationχ2 = 18.68; p < 0.0001
Control vs. P93 ablationχ2 = 8.0; p = 0.0047
b1GNormal distributionTwo-way ANOVA; Tukey post hoc analysisP13 ablation: body weight
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp = 0.4746
Control 10 dpi vs. DKO 10 dpip = 0.1806
Control 20 dpi vs. DKO 20 dpip = 0.0038
Control 30 dpi vs. DKO 30 dpip = 0.0023
c1HNormal distributionTwo-way ANOVA; Tukey post hoc analysisP23 ablation: body weight
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0457
Genotype × timing interactionp = 0.0002
Control 10 dpi vs. DKO 10 dpip = 0.9953
Control 20 dpi vs. DKO 20 dpip = 1943
Control 60 dpi vs. DKO 60 dpip < 0.0001
d1INormal distributionTwo-way ANOVA; Tukey post hoc analysisP93 ablation: body weight
Effect of genotypep = 1410
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp = 0.2562
Control 10 dpi vs. DKO 10 dpip > 0.9999
Control 20 dpi vs. DKO 20 dpip = 0.9996
Control 1200 dpi vs. DKO 120 dpip = 0.3155
e2J, AnkGNormal distributionTwo-way ANOVA; Tukey post hoc analysisSN P13 ablation: AnkG nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. Ank cKO 10 dpip = 0.0694
Control 20 dpi vs. Ank cKO 20 dpip < 0.0001
Control 30 dpi vs. Ank cKO 30 dpip < 0.0001
Control 10 dpi vs. DKO 10 dpip < 0.0001
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip < 0.0001
Ank cKO 10 dpi vs. 20 dpip = 0.0010
Ank cKO 10 dpi vs. 30 dpip < 0.0001
Ank cKO 20 dpi vs. 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip > 0.9999
DKO 10 dpi vs. 30 dpip = 0.9997
DKO 20 dpi vs. 30 dpip > 0.9999
f2J, NF186Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P13 ablation: NF186 nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 30 dpi vs. Ank cKO 30 dpip > 0.9999
Control 10 dpi vs. DKO 10 dpip = 0.9984
Control 20 dpi vs. DKO 20 dpip = 0.0015
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.0042
DKO 10 dpi vs. 30 dpip < 0.0001
DKO 20 dpi vs. 30 dpip < 0.0001
g2J′, AnkGNormal distributionTwo-way ANOVA; Tukey post hoc analysisSC P13 ablation: AnkG nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. Ank cKO 10 dpip < 0.0001
Control 20 dpi vs. Ank cKO 20 dpip < 0.0001
Control 30 dpi vs. Ank cKO 30 dpip < 0.0001
Control 10 dpi vs. DKO 10 dpip < 0.0001
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip < 0.0001
Ank cKO 10 dpi vs. 20 dpip = 0.0011
Ank cKO 10 dpi vs. 30 dpip < 0.0001
Ank cKO 20 dpi vs. 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.9973
DKO 10 dpi vs. 30 dpip > 0.9998
DKO 20 dpi vs. 30 dpip = 0.9996
h2J′, NF186Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P13 ablation: NF186 nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 30 dpi vs. Ank cKO 30 dpip > 0.9999
Control 10 dpi vs. DKO 10 dpip = 0.5624
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.0001
DKO 10 dpi vs. 30 dpip < 0.0001
DKO 20 dpi vs. 30 dpip < 0.0001
i2K, AnkGNormal distributionTwo-way ANOVA; Tukey post hoc analysisSN P23 ablation: AnkG nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. Ank cKO 10 dpip = 0.9389
Control 20 dpi vs. Ank cKO 20 dpip < 0.0001
Control 60 dpi vs. Ank cKO 60 dpip < 0.0001
Control 10 dpi vs. DKO 10 dpip < 0.0001
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 60 dpi vs. DKO 60 dpip < 0.0001
Ank cKO 10 dpi vs. 20 dpip = 0.0008
Ank cKO 10 dpi vs. 60 dpip < 0.0001
Ank cKO 20 dpi vs. 60 dpip < 0.0001
DKO 10 dpi vs. 20 dpip > 0.9999
DKO 10 dpi vs. 60 dpip > 0.9999
DKO 20 dpi vs. 60 dpip = 0.8804
j2K, NF186Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P23 ablation: NF186 nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 60 dpi vs. Ank cKO 60 dpip > 0.9999
Control 10 dpi vs. DKO 10 dpip = 0.9943
Control 20 dpi vs. DKO 20 dpip = 0.0031
Control 60 dpi vs. DKO 60 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.0089
DKO 10 dpi vs. 60 dpip < 0.0001
DKO 20 dpi vs. 60 dpip < 0.0001
k2K′, AnkGNormal distributionTwo-way ANOVA; Tukey post hoc analysisSC P23 ablation: AnkG nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. Ank cKO 10 dpip < 0.0001
Control 20 dpi vs. Ank cKO 20 dpip < 0.0001
Control 60 dpi vs. Ank cKO 60 dpip < 0.0001
Control 10 dpi vs. DKO 10 dpip < 0.0001
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 60 dpi vs. DKO 60 dpip < 0.0001
Ank cKO 10 dpi vs. 20 dpip < 0.0001
Ank cKO 10 dpi vs. 60 dpip < 0.0001
Ank cKO 20 dpi vs. 60 dpip < 0.0001
DKO 10 dpi vs. 20 dpip > 0.9999
DKO 10 dpi vs. 60 dpip > 0.9999
DKO 20 dpi vs. 60 dpip = 0.9991
l2K′, NF186Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P23 ablation: NF186 nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 60 dpi vs. Ank cKO 60 dpip > 0.9999
Control 10 dpi vs. DKO 10 dpip = 0.6324
Control 20 dpi vs. DKO 20 dpip = 0.0001
Control 60 dpi vs. DKO 60 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.0009
DKO 10 dpi vs. 60 dpip < 0.0001
DKO 20 dpi vs. 60 dpip < 0.0001
m2L, AnkGNormal distributionTwo-way ANOVA; Tukey post hoc analysisSN P93 ablation: AnkG nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.9562
Genotype × timing interactionp = 0.1124
Control 10 dpi vs. DKO 10 dpip < 0.0001
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 120 dpi vs. DKO 120 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.7683
DKO 10 dpi vs. 120 dpip = 0.9502
DKO 20 dpi vs. 120 dpip = 0.9925
n2L, NF186Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P93 ablation: NF186 nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.1372
Control 20 dpi vs. DKO 20 dpip = 0.0182
Control 120 dpi vs. DKO 120 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.9704
DKO 10 dpi vs. 120 dpip < 0.0001
DKO 20 dpi vs. 120 dpip < 0.0001
o2L′, AnkGNormal distributionTwo-way ANOVA; Tukey post hoc analysisSC P93 ablation: AnkG nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0083
Genotype × timing interactionp = 0.0063
Control 10 dpi vs. DKO 10 dpip < 0.0001
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 120 dpi vs. DKO 120 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.1040
DKO 10 dpi vs. 120 dpip = 0.0010
DKO 20 dpi vs. 120 dpip = 0.2210
p2L′, NF186Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P93 ablation: NF186 nodal count
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.9167
Control 20 dpi vs. DKO 20 dpip = 0.4298
Control 120 dpi vs. DKO 120 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.9131
DKO 10 dpi vs. 120 dpip < 0.0001
DKO 20 dpi vs. 120 dpip < 0.0001
q2M, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P13 ablation: NF186 intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0001
Genotype × timing interactionp = 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.4702
Control 20 dpi vs. DKO 20 dpip = 0.0001
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.0125
DKO 10 dpi vs. 30 dpip < 0.0001
DKO 20 dpi vs. 30 dpip = 0.0036
r2M, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P23 ablation: NF186 intensity
Effect of genotypep = 0.0007
Effect of timing postinjectionp = 0.0308
Genotype × timing interactionp = 0.0308
Control 10 dpi vs. DKO 10 dpip = 0.9972
Control 20 dpi vs. DKO 20 dpip = 0.2550
Control 60 dpi vs. DKO 60 dpip = 0.0029
DKO 10 dpi vs. 20 dpip = 0.5495
DKO 10 dpi vs. 60 dpip = 0.0070
DKO 20 dpi vs. 60 dpip = 0.1800
s2M, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P93 ablation: NF186 intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip > 0.9999
Control 20 dpi vs. DKO 20 dpip = 0.2330
Control 120 dpi vs. DKO 120 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.2696
DKO 10 dpi vs. 120 dpip < 0.0001
DKO 20 dpi vs. 120 dpip = 0.0003
t2M′, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P13 ablation: NF186 intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0003
Genotype × timing interactionp = 0.0003
Control 10 dpi vs. DKO 10 dpip = 0.9382
Control 20 dpi vs. DKO 20 dpip = 0.0971
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.5018
DKO 10 dpi vs. 30 dpip < 0.0001
DKO 20 dpi vs. 30 dpip = 0.0009
u2M′, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P23 ablation: NF186 intensity
Effect of genotypep = 0.0013
Effect of timing postinjectionp = 0.0112
Genotype × timing interactionp = 0.0113
Control 10 dpi vs. DKO 10 dpip > 0.9999
Control 20 dpi vs. DKO 20 dpip = 0.5115
Control 60 dpi vs. DKO 60 dpip = 0.0010
DKO 10 dpi vs. 20 dpip = 0.6657
DKO 10 dpi vs. 60 dpip = 0.0028
DKO 20 dpi vs. 60 dpip = 0.0329
v2M′, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P93 ablation: NF186 intensity
Effect of genotypep = 0.0007
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip > 0.9999
Control 20 dpi vs. DKO 20 dpip = 0.9996
Control 120 dpi vs. DKO 120 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.9978
DKO 10 dpi vs. 120 dpip < 0.0001
DKO 20 dpi vs. 120 dpip < 0.0001
w2QNormal distributionUnpaired, two-tailed t testSN P13 ablation: relative protein level
AnkG level: control vs. Ank cKOp = 0.0002
AnkG level: control vs. DKOp = 0.006
NF186 level: control vs. Ank cKOp = 0.9415
NF186 level: control vs. DKOp = 0.0062
×2Q′Normal distributionUnpaired, two-tailed t testSC P13 ablation: relative protein level
AnkG level: control vs. Ank cKOp = 0.0079
AnkG level: control vs. DKOp = 0.0013
NF186 level: control vs. Ank cKOp = 0.355
NF186 level: control vs. DKOp = 0.0003
y2RNormal distributionUnpaired, two-tailed t testSN P23 ablation: relative protein level
AnkG level: control vs. Ank cKOp = 0.0404
AnkG level: control vs. DKOp = 0.0046
NF186 level: control vs. Ank cKOp = 0.3541
NF186 level: control vs. DKOp = 0.0249
z2R′Normal distributionUnpaired, two-tailed t testSC P23 ablation: relative protein level
AnkG level: control vs. Ank cKOp = 0.0036
AnkG level: control vs. DKOp = 0.0016
NF186 level: control vs. Ank cKOp = 0.7327
NF186 level: control vs. DKOp = 0.0066
aa2SNormal distributionUnpaired, two-tailed t testSN P93 ablation: relative protein level
AnkG level: control vs. DKOp = 0.0043
NF186 level: control vs. DKOp < 0.0001
ab2S′Normal distributionUnpaired, two-tailed t testSC P93 ablation: relative protein level
AnkG level: control vs. DKOp = 0.0009
NF186 level: control vs. DKOp = 0.0002
ac3S, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P13 ablation: NaV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.9992
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip < 0.0001
DKO 10 dpi vs. 30 dpip < 0.0001
DKO 20 dpi vs. 30 dpip < 0.0001
ad3S, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P23 ablation: NaV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0002
Genotype × timing interactionp = 0.0002
Control 10 dpi vs. DKO 10 dpip = 0.9996
Control 20 dpi vs. DKO 20 dpip = 0.0003
Control 60 dpi vs. DKO 60 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.0011
DKO 10 dpi vs. 60 dpip < 0.0001
DKO 20 dpi vs. 60 dpip = 0.93009
ae3S, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P93 ablation: NaV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0027
Genotype × timing interactionp = 0.0027
Control 10 dpi vs. DKO 10 dpip = 0.9996
Control 20 dpi vs. DKO 20 dpip = 0.0058
Control 120 dpi vs. DKO 120 dpip = 0.0012
DKO 10 dpi vs. 20 dpip = 0.0032
DKO 10 dpi vs. 120 dpip = 0.0004
DKO 20 dpi vs. 120 dpip = 0.6551
af3T, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P13 ablation: NaV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.3498
Genotype × timing interactionp = 0.3510
Control 10 dpi vs. DKO 10 dpip = 0.0085
Control 20 dpi vs. DKO 20 dpip = 0.0018
Control 30 dpi vs. DKO 30 dpip = 0.0003
DKO 10 dpi vs. 20 dpip = 0.9713
DKO 10 dpi vs. 30 dpip = 0.2965
DKO 20 dpi vs. 30 dpip = 0.7138
ag3T, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P23 ablation: NaV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0451
Genotype × timing interactionp = 0.0452
Control 10 dpi vs. DKO 10 dpip = 0.4863
Control 20 dpi vs. DKO 20 dpip = 0.0089
Control 60 dpi vs. DKO 60 dpip = 0.0004
DKO 10 dpi vs. 20 dpip = 0.2164
DKO 10 dpi vs. 60 dpip = 0.0152
DKO 20 dpi vs. 60 dpip = 0.8617
ah3T, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P93 ablation: NaV intensity
Effect of genotypep = 0.0015
Effect of timing postinjectionp = 0.0108
Genotype × timing interactionp = 0.0107
Control 10 dpi vs. DKO 10 dpip = 0.9987
Control 20 dpi vs. DKO 20 dpip = 0.7937
Control 120 dpi vs. DKO 120 dpip = 0.0025
DKO 10 dpi vs. 20 dpip = 0.9066
DKO 10 dpi vs. 120 dpip = 0.0026
DKO 20 dpi vs. 120 dpip = 0.0259
ai3UNormal distributionUnpaired, two-tailed t testSC- NaV: relative protein level
Control vs. P13 ablation DKOp = 0.8532
Control vs. P23 ablation DKOp = 0.927
Control vs. P93 ablationp = 0.98
aj4S, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P13 ablation: BIV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0169
Genotype × timing interactionp = 0.0170
Control 10 dpi vs. DKO 10 dpip = 0.1170
Control 20 dpi vs. DKO 20 dpip = 0.0017
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.1209
DKO 10 dpi vs. 30 dpip = 0.0036
DKO 20 dpi vs. 30 dpip = 0.6801
ak4S, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P23 ablation: BIV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0423
Genotype × timing interactionp = 0.0425
Control 10 dpi vs. DKO 10 dpip = 0.7035
Control 20 dpi vs. DKO 20 dpip = 0.1771
Control 60 dpi vs. DKO 60 dpip = 0.0006
DKO 10 dpi vs. 20 dpip = 0.9089
DKO 10 dpi vs. 60 dpip = 0.0215
DKO 20 dpi vs. 60 dpip = 0.1587
al4S, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P93 ablation: BIV intensity
Effect of genotypep = 0.0006
Effect of timing postinjectionp = 0.0394
Genotype × timing interactionp = 0.0394
Control 10 dpi vs. DKO 10 dpip = 0.9642
Control 20 dpi vs. DKO 20 dpip = 0.2846
Control 120 dpi vs. DKO 120 dpip = 0.0040
DKO 10 dpi vs. 20 dpip = 0.6927
DKO 10 dpi vs. 120 dpip = 0.0147
DKO 20 dpi vs. 120 dpip = 0.1642
am4T, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P13 ablation: BIV intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.2569
Genotype × timing interactionp = 0.2569
Control 10 dpi vs. DKO 10 dpip = 0.0004
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.9939
DKO 10 dpi vs. 30 dpip = 0.2390
DKO 20 dpi vs. 30 dpip = 0.5023
an4T, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P23 ablation: BIV intensity
Effect of genotypep = 0.0003
Effect of timing postinjectionp = 0.1459
Genotype × timing interactionp = 0.1459
Control 10 dpi vs. DKO 10 dpip = 0.8248
Control 20 dpi vs. DKO 20 dpip = 0.1930
Control 60 dpi vs. DKO 60 dpip = 0.0043
DKO 10 dpi vs. 20 dpip = 0.8728
DKO 10 dpi vs. 60 dpip = 0.0925
DKO 20 dpi vs. 60 dpip = 0.5341
ao4T, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P93 ablation: BIV intensity
Effect of genotypep = 0.0198
Effect of timing postinjectionp = 0.0050
Genotype × timing interactionp = 0.0050
Control 10 dpi vs. DKO 10 dpip > 0.9999
Control 20 dpi vs. DKO 20 dpip = 0.9978
Control 120 dpi vs. DKO 120 dpip = 0.0042
DKO 10 dpi vs. 20 dpip = 0.9877
DKO 10 dpi vs. 120 dpip = 0.0031
DKO 20 dpi vs. 120 dpip = 0.0019
ap4UNormal distributionUnpaired, two-tailed t testSC- BIV: relative protein level
Control vs. P13 ablation DKOp = 0.7971
Control vs. P23 ablation DKOp = 0.9601
Control vs. P93 ablationp = 0.8546
aq5S, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P13 ablation: AnkR intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0001
Genotype × timing interactionp = 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.0005
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip > 0.9999
DKO 10 dpi vs. 20 dpip = 0.0926
DKO 10 dpi vs. 30 dpip = 0.0009
DKO 20 dpi vs. 30 dpip < 0.0001
ar5S, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P23 ablation: AnkR intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip < 0.0001
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 60 dpi vs. DKO 60 dpip = 0.8245
DKO 10 dpi vs. 20 dpip < 0.0001
DKO 10 dpi vs. 60 dpip < 0.0001
DKO 20 dpi vs. 60 dpip < 0.0001
as5S, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSN P93 ablation: AnkR intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.0008
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 120 dpi vs. DKO 120 dpip = 0.1776
DKO 10 dpi vs. 20 dpip < 0.0001
DKO 10 dpi vs. 120 dpip < 0.0001
DKO 20 dpi vs. 120 dpip < 0.0001
at5T, P13Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P13 ablation: AnkR intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0002
Genotype × timing interactionp < 0.0003
Control 10 dpi vs. DKO 10 dpip = 0.3295
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip = 0.9979
DKO 10 dpi vs. 20 dpip < 0.0001
DKO 10 dpi vs. 30 dpip = 0.4570
DKO 20 dpi vs. 30 dpip < 0.0001
au5T, P23Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P23 ablation: AnkR intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.8577
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 60 dpi vs. DKO 60 dpip > 0.9999
DKO 10 dpi vs. 20 dpip < 0.0001
DKO 10 dpi vs. 60 dpip = 0.8179
DKO 20 dpi vs. 60 dpip < 0.0001
av5T, P93Normal distributionTwo-way ANOVA; Tukey post hoc analysisSC P93 ablation: AnkR intensity
Effect of genotypep < 0.0001
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.8502
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 120 dpi vs. DKO 120 dpip = 0.4611
DKO 10 dpi vs. 20 dpip < 0.0001
DKO 10 dpi vs. 120 dpip = 0.9796
DKO 20 dpi vs. 120 dpip < 0.0001
aw5UNormal distributionUnpaired, two-tailed t testSC- AnkR: relative protein level
Control vs. P13 ablation DKOp = 0.9677
Control vs. P23 ablation DKOp = 0.4293
Control vs. P93 ablationp = 0.2763
ax6BNormal distributionTwo-way ANOVA; Tukey post hoc analysisP13 ablation: NCV
Effect of genotypep = 0.0927
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp < 0.0001
Control 10 dpi vs. DKO 10 dpip = 0.0005
Control 20 dpi vs. DKO 20 dpip < 0.0001
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.7586
DKO 10 dpi vs. 30 dpip = 0.2018
DKO 20 dpi vs. 30 dpip = 0.9264
ay6CNormal distributionTwo-way ANOVA; Tukey post hoc analysisP13 ablation: Notch Amp
Effect of genotypep = 0.0006
Effect of timing postinjectionp < 0.0001
Genotype × timing interactionp = 0.0016
Control 10 dpi vs. DKO 10 dpip = 0.9992
Control 20 dpi vs. DKO 20 dpip = 0.3675
Control 30 dpi vs. DKO 30 dpip < 0.0001
DKO 10 dpi vs. 20 dpip = 0.7221
DKO 10 dpi vs. 30 dpip = 0.9993
DKO 20 dpi vs. 30 dpip = 0.4799
az6ENormal distributionTwo-way ANOVA; Tukey post hoc analysisP23 ablation: NCV
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.0428
Genotype × timing interactionp = 0.0171
Control 10 dpi vs. DKO 10 dpip > 0.9999
Control 20 dpi vs. DKO 20 dpi0.0021
Control 60 dpi vs. DKO 60 dpi0.0004
DKO 10 dpi vs. 20 dpip > 0.9999
DKO 10 dpi vs. 60 dpip = 0.9991
DKO 20 dpi vs. 60 dpip = 0.9998
ba6FNormal distributionTwo-way ANOVA; Tukey post hoc analysisP23 ablation: Notch Amp
Effect of genotypep = 0.0007
Effect of timing postinjectionp = 0.5107
Genotype × timing interactionp = 0.4310
Control 10 dpi vs. DKO 10 dpip = 0.9582
Control 20 dpi vs. DKO 20 dpip = 0.0492
Control 60 dpi vs. DKO 60 dpip = 0.034
DKO 10 dpi vs. 20 dpip = 0.6092
DKO 10 dpi vs. 60 dpip = 0.8662
DKO 20 dpi vs. 60 dpip = 0.9906
bb6HNormal distributionTwo-way ANOVA; Tukey post hoc analysisP93 ablation: NCV
Effect of genotypep < 0.0001
Effect of timing postinjectionp = 0.9269
Genotype × timing interactionp = 0.0770
Control 20 dpi vs. DKO 20 dpip = 0.8318
Control 60 dpi vs. DKO 60 dpip = 0.0002
Control 120 dpi vs. DKO 120 dpip = 0.0644
DKO 20 dpi vs. 60 dpip = 0.9163
DKO 20 dpi vs. 120 dpip = 0.4341
DKO 60 dpi vs. 120 dpip = 0.9652
bc6INormal distributionTwo-way ANOVA; Tukey post hoc analysisP93 ablation: Notch Amp
Effect of genotypep = 0.0213
Effect of timing postinjectionp = 0.0088
Genotype × timing interactionp = 0.0174
Control 20 dpi vs. DKO 20 dpip > 0.9999
Control 60 dpi vs. DKO 60 dpip = 0.0713
Control 120 dpi vs. DKO 120 dpip = 0.0041
DKO 20 dpi vs. 60 dpip > 0.9999
DKO 20 dpi vs. 120 dpip = 0.3463
DKO 60 dpi vs. 120 dpip = 0.4148

  • Note: GraphPad Prism, which was used to perform all statistical analyses, does not report exact p-values higher than 0.9999 or lower than 0.0001.

  • All statistical tests are summarized with exact p-values.