Table 1.

Statistical analysis

Location	Data structure	Type of test	CI/power	P value	Comparison
a	Normally distributed	One-way ANOVA	0.97	<0.0001	Grp expression in midbrain subregions
	Normally distributed	Tukey’s post hoc	6.508 to 17.74	<0.0001	Grp IF vs. PN/PIF
	Normally distributed	Tukey’s post hoc	34.77 to 46	<0.0001	Grp IF vs. PBP
	Normally distributed	Tukey’s post hoc	35.1 to 46.33	<0.0001	Grp IF vs. SNc-V
	Normally distributed	Tukey’s post hoc	57.26 to 68.49	<0.0001	Grp IF vs. SNc-D
	Normally distributed	Tukey’s post hoc	22.65 to 33.88	<0.0001	Grp PN/PIF vs. PBP
	Normally distributed	Tukey’s post hoc	22.97 to 34.2	<0.0001	Grp PN/PIF vs. SNc-V
	Normally distributed	Tukey’s post hoc	45.13 to 56.37	<0.0001	Grp PN/PIF vs. SNc-D
	Normally distributed	Tukey’s post hoc	–5.292 to 5.942	0.9998	Grp PBP vs. SNc-V
	Normally distributed	Tukey’s post hoc	16.87 to 28.1	<0.0001	Grp PBP vs. SNc-D
	Normally distributed	Tukey’s post hoc	16.55 to 27.78	<0.0001	Grp SNc-V vs. SNcD
b	Normally distributed	One-way ANOVA	0.96	<0.0001	Neurod6 expression in midbrain subregions
	Normally distributed	Tukey’s post hoc	–0.4177 to 9.918	0.0842	Neurod6 IF vs. PN/PIF
	Normally distributed	Tukey’s post hoc	16.28 to 26.62	<0.0001	Neurod6 IF vs. PBP
	Normally distributed	Tukey’s post hoc	34.69 to 45.03	<0.0001	Neurod6 IF vs. SNc-V
	Normally distributed	Tukey’s post hoc	35.82 to 46.16	<0.0001	Neurod6 IF vs. SNc-D
	Normally distributed	Tukey’s post hoc	11.53 to 21.87	<0.0001	Neurod6 PN/PIF vs.
	Normally distributed	Tukey’s post hoc			PBP
	Normally distributed	Tukey’s post hoc	29.94 to 40.28	<0.0001	Neurod6 PN/PIF vs. SNc-V
	Normally distributed	Tukey’s post hoc	31.07 to 41.41	<0.0001	Neurod6 PN/PIF vs. SNc-D
	Normally distributed	Tukey’s post hoc	13.24 to 23.58	<0.0001	Neurod6 PBP vs. SNc-V
	Normally distributed	Tukey’s post hoc	14.37 to 24.71	<0.0001	Neurod6 PBP vs. SNc-D
	Normally distributed	Tukey’s post hoc	–4.043 to 6.293	0.9699	Neurod6 SNc-V vs. SNc-D
c	Normally distributed	One-way ANOVA	0.522	0.036	Grp expression by age
	Normally distributed	Tukey’s post hoc	–1.112 to 2.462	0.5636	Grp 2 wk vs. 8 wk
	Normally distributed	Tukey’s post hoc	–3.087 to 0.4872	0.1603	Grp 2 wk vs. 16 wk
	Normally distributed	Tukey’s post hoc	–3.762 to – 0.1878	0.0316	Grp 8 wk vs. 16 wk
d	Normally distributed	One-way ANOVA	0.56	0.0236	Neurod6 expression by age
	Normally distributed	Tukey’s post hoc	–2.859 to 1.109	0.4655	Neurod6 2 wk vs. 8 wk
	Normally distributed	Tukey’s post hoc	–4.384 to – 0.4162	0.0201	Neurod6 2 wk vs. 16 wk
	Normally distributed	Tukey’s post hoc	–3.509 to 0.4588	0.1351	Neurod6 8 wk vs. 16 wk
e	Normally distributed	Unpaired t test	–7.68 to 3.147	0.3728	Grp IF M vs. F
f	Normally distributed	Unpaired t test	–12.78 to 0.4155	0.0634	Grp PN/PIF M vs. F
g	Normally distributed	Unpaired t test	–1.924 to 4.89	0.3549	Grp PBP M vs. F
h	Normally distributed	Unpaired t test	–7.321 to 5.188	0.7119	Grp SNCv M vs. F
i	Normally distributed	Unpaired t test	–0.6657 to 1.199	0.5383	Grp SNCd M vs. F
j	Normally distributed	Unpaired t test	–13.48 to 4.412	0.2852	Neurod6 IF M vs. F
k	Normally distributed	Unpaired t test	–8.615 to 3.682	0.3924	Neurod6 PN/PIF M vs. F
l	Normally distributed	Unpaired t test	–1.028 to 2.095	0.4642	Neurod6 PBP M vs. F
m	Normally distributed	Unpaired t test	–2.251 to 0.1514	0.0801	Neurod6 SNCv M vs. F
n	Negative binomial	Likelihood ratio test	n/a	0.5113	Neurod6 M vs. F RNASeq data
o	Negative binomial	Likelihood ratio test	n/a	0.6835	Grp M vs. F RNASeq data
p	Normally distributed	Unpaired t test	–9.156 to –1.532	0.0075	Vm – SNc vs. NEXCre
q	Normally distributed	Unpaired t test	–1222 to –729.4	<0.0001	Rm – SNc vs. NEXCre
r	Normally distributed	Unpaired t test	23.67 to 43.99	<0.0001	Cm – SNc vs. NEXCre
s	Normally distributed	Unpaired t test	11.3 to 21.88	<0.0001	AP height – SNc vs. NEX-Cre
t	Normally distributed	Unpaired t test	3.372 to 9.524	0.0002	AHP – SNc vs. NEXCre
u	Normally distributed	Unpaired t test	6.564 to 10.49	<0.0001	Sag – SNc vs. NEXCre
v	Normally distributed	Unpaired t test	4.295 to 7.773	<0.0001	Rebound – SNc vs. NEX-Cre
w	Normally distributed	One-way ANOVA	0.85	0.0002	Cylinder test
	Normally distributed	Tukey’s post hoc	6.33 to 55.07	0.0163	Saline vs. 6-OHDA paw
	Normally distributed	Tukey’s post hoc	–55.27 to –6.53	0.0157	Saline vs. both paws
	Normally distributed	Tukey’s post hoc	–85.97 to –37.23	0.0002	6-OHDA vs. both paws
x	Normally distributed	Paired one-way ANOVA	0.96	<0.0001	Cell population survival
	Normally distributed	Dunnett’s post hoc	–69.31 to –34.69	0.0022	All TH⁺ vs. Grp-/ Neurod6 ⁺
	Normally distributed	Dunnett’s post hoc	–6.01 to 15.16	0.3169	All TH⁺ vs. Grp ⁺/ Neurod6 ⁺
	Normally distributed	Dunnett’s post hoc	2.07 to 15.78	0.0246	All TH⁺ vs. Grp⁺/ Neurod6-
	Normally distributed	Dunnett’s post hoc	0.6149 to 15.14	0.0403	All TH⁺ vs. Lypd1 ⁺/Neurod6-
y	Normally distributed	Paired t test	–3.593 to 3.043	0.8091	Lypd1⁺/Neurod6 ⁺ saline vs. 6-OHDA