Table 1:

Summary of CaMKIIa optogenetic stimulations experiments

AAV expressionEvoked EPCs
PmCoBAOTMCGCmac
YesN/DNoYesNo
NoN/DYesN/D
NoN/DYesNo
YesN/DYesNo
YesN/DYesYesYes
YesNoN/DYes
YesNoN/DYes
YesNoN/DNo
YesNoN/DN/DYes
YesNoNoYes
YesNoNoYes
YesNoNoN/D
YesNoNoNo
NoNoNoNo
YesNoNoN/D
NoNoNoNo
YesNoNoYes
YesNoNoYes
YesNoNoN/DYes
YesNoNoYes
YesYesN/DN/D
YesYesYesNo
YesYesYesN/D
YesYesYesN/D
YesYesYesYes
YesYesYesN/DYes
YesYesYesYesYes
YesYesYesN/DYes
  • Viral delivery of Chr2 to the PmCO was accomplished using a CaMKIIa-specific viral vector (see Materials and Methods) to target excitatory neurons. When the PmCo was efficiently targeted (first left column, PmCo, value = yes), excitatory responses were evoked in AOB GCs. Similar responses were observed also in AOB MCs (MC) but only when the BAOT was labeled (second left column, BAOT, value = yes; N = 7). In other cases (N = 4) MCs were also responsive to perisomatic light stimulations but, due to tissue damage during brain tissue harvest, the area corresponding to the anterior portion of the injection site (in proximity with the BAOT) was not available. This survey was used to assess the likelihood of PmCo-to-GC and BAOT-to-MC connectivity.