Parameters of spontaneous EPSCs in SCN neurons
| Genotype | Mice (n) | Neurons (n) | Amplitude (pA) | Rise time (10–90% of amplitude) (ms) | Decay time constant (ms) | Area (charge) (pAm) | Frequency events/s |
|---|---|---|---|---|---|---|---|
| WT | 10 | 28 | 14.4 ± 0.9 | 1.9 ± 0.1 | 2.9 ± 0.2 | 46.2 ± 4.6 | 2.6 ± 0.8 |
| Control | 3 | 11 | 17.1 ± 1.7 | 1.4 ± 0.2 | 3.6 ± 0.2 | 43.9 ± 4.0 | 1.9 ± 0.7 |
| cKO | 10 | 15 | 16.3 ± 1.1 | 1.6 ± 0.2 | 2.9 ± 0.2 | 46.3 ± 3.4 | 2.4 ± 0.7 |
| ANOVA F-crit | 3.18 | 3.18 | 3.18 | 3.18 | 3.18 | ||
| ANOVA F(2,51) | 1.44 | 4.11 | 0.55 | 0.06 | 0.12 | ||
| p, (cKO vs WT) | 0.47 | 0.21 | 0.95 | 1.00 | 0.99 | ||
| p, (cKO vs control) | 0.91 | 0.53 | 0.78 | 0.95 | 0.92 | ||
| p, (control vs WT) | 0.29 | 0.02* | 0.56 | 0.95 | 0.86 |
WT: wild-type controls; control: littermate controls (Opn4+/+); cKO: Opn4Cre/+; Vglut2loxP/loxP. *p < 0.05, one-way ANOVA followed by Tukey HSD post hoc test (Excel, Igor Pro). MiniAnalysis software (Synaptosoft Inc.) was used for acquisition and analysis of spontaneous EPSCs parameters.