Figure | Statistical test | N | Statistical significance |
---|---|---|---|

1E | Unpaired t test, two-tailed | TMPase: N = 6 | TMPase: p < 0.0001 |

Cell area | CGRP: N = 5 | CGRP+,NF200-: p < 0.0001 | |

CGRP+,NF200+: p = 0.4803 | |||

Table 3 | Unpaired t test, two-tailed | N = 5–6 | See Table 3 |

Cell distribution | |||

2B | Unpaired t test, two-tailed | Adult: N = 6 | Adult control vs adult Nav-Tsc2: p = 0.0047 |

Axon diameter | P29: N = 5 | P29 control vs P29 Nav-Tsc2: p = 0.0442 | |

Adult control vs P29 control: p = 0.318 | |||

Adult Nav-Tsc2 vs P29 Nav-Tsc2: p = 0.0258 | |||

2C | Unpaired t test, two-tailed | Adult: N = 6 | Adult control vs adult Nav-Tsc2: p = 0.0055 |

Axons >1 micron | P29: N = 5 | P29 control vs P29 Nav-Tsc2: p = 0.2076 | |

Adult control vs P29 control: p = 0.4211 | |||

Adult Nav-Tsc2 vs P29 Nav-Tsc2: p = 0.0125 | |||

2D | Unpaired t test, two-tailed | Adult: N = 6 | Adult control vs adult Nav-Tsc2: p = 0.0371 |

Axons/bundle | P29: N = 5 | P29 control vs P29 Nav-Tsc2: p = 0.0747 | |

Adult control vs P29 control: p = 0.5018 | |||

Adult Nav-Tsc2 vs P29 Nav-Tsc2: p = 0.0202 | |||

3C | Unpaired t test, two-tailed | N = 5 | TuJ1: p < 0.0001 |

Skin innervation | |||

3F | Unpaired t test, two-tailed | N = 5 | Lamina I: p = 0.0403 |

GFP density | Lamina II: p = 0.0002 | ||

5C,F,G,L | Unpaired t test, two-tailed | N = 5 | Total neurons: p = 0.081 |

DRG neuron | SP: N = 6 | NF200 total: p = 0.314 | |

counting | SP total: p = 0.0006 | ||

CGRP+,NF200-: p < 0.0001 | |||

CGRP+,NF200+: p = 0.0007 | |||

TrkA+,NF200-: p < 0.0001 | |||

TrkA+,NF200+: p = 0.0005 | |||

IB4 total: p = 0.0001 | |||

IB4+,NF200+: p = 0.0037 | |||

6C | Unpaired t test, two-tailed | N = 4 whole DRG | Nav1.8: p = 0.0613 |

qPCR of | N = 3 FACS-sorted samples | TrkA: p = 0.0015 | |

FACS-sorting | TrkB: p = 0.9758 | ||

TrkC: p = 0.0020 | |||

Prx: p = 0.1784 | |||

Egr2: p = 0.5834 | |||

8A | Unpaired t test, two-tailed | Female: N = 13 | Female: p = 0.2513 |

von Frey | Male: N = 12 | Male: p = 0.462 | |

8B | Unpaired t test, two-tailed | Female: N = 6 control, N = 4 cKO | Female: p = 0.2390 |

Cold plantar | male: N = 11 control, N = 10 cKO | Male: p = 0.2102 | |

8C | Unpaired t test, two-tailed | Female: N = 13 | Female: p = 0.0046 |

Hargreaves | Male: N = 9 | Male: p = 0.0337 | |

8D | Two-way RM ANOVA | Control: N = 9 | Interaction: F_{(5,75)} = 1.82; p = 0.1191 |

Female CCI | Nav-Tsc2: N = 8 | Time: F_{(5,75)} = 10.04; p < 0.0001 | |

Genotype: F_{(1,15)} = 9.032; p = 0.0089 | |||

8D | Sidak’s multiple | Control: N = 9 | BL: adjusted p > 0.9999 |

Female CCI | comparison test | Nav-Tsc2: N = 8 | POD 4: adjusted p = 0.269 |

POD 6: adjusted p = 0.9896 | |||

POD 20: adjusted p = 0.9209 | |||

POD 27: adjusted p = 0.0071 | |||

POD 34: adjusted p = 0.0836 | |||

8E | Two-way RM ANOVA | Control: N = 11 | Interaction: F_{(5,75)} = 0.7719; p = 0.5723 |

Male CCI | Nav-Tsc2: N = 10 | Time: F_{(5,95)} = 4.873; p = 0.0005 | |

Genotype: F_{(1,19)} = 3.403; p = 0.0807 | |||

8E | Sidak’s multiple | Control: N = 11 | BL: adjusted p > 0.9999 |

Male CCI | comparison test | Nav-Tsc2: N = 10 | POD 4: adjusted p = 0.9992 |

POD 6: adjusted p = 0.9998 | |||

POD 20: adjusted p = 0.3652 | |||

POD 27: adjusted p = 0.236 | |||

POD 34: adjusted p = 0.8045 | |||

8F | Two-way RM ANOVA | Control: N = 17 | Interaction: F_{(4,128)} = 1.03; p = 0.3946 |

Rotarod | Nav-Tsc2: N = 17 | Trial number: F_{(4,128)} = 60.26; p < 0.0001 | |

Genotype: F_{(1,32)} = 4.08; p = 0.0518 | |||

8F | Sidak’s multiple | Control: N = 17 | Trial 1: adjusted p = 0.8576 |

Rotarod | comparison test | Nav-Tsc2: N = 17 | Trial 2: adjusted p = 0.8396 |

Trial 3: adjusted p = 0.2686 | |||

Trial 4: adjusted p = 0.0812 | |||

Trial 5: adjusted p = 0.1971 | |||

8G | Unpaired t test, two-tailed | Control: N = 23 | p = 0.8027 |

Open field activity | Nav-Tsc2: N = 23 | ||

8H | Two-way RM ANOVA | Control: N = 23 | Interaction: F_{(1,43)} = 2.62; p = 0.1129 |

Pole climb down | Nav-Tsc2: N = 22 | Trial number: F_{(1,43)} = 4.516; p = 0.0394 | |

Genotype: F_{(1,43)} = 0.04254; p = 0.8376 | |||

8H | Sidak’s multiple | Control: N = 23 | Trial 1: adjusted p = 0.84 |

Pole climb down | comparison test | Nav-Tsc2: N = 22 | Trial 2: adjusted p = 0.606 |