Table 2.

Gephyrin E-domain binding enthalpy and binding entropy of GlyR β-loop wild-type determined by ITC

Parameterβ-Loop variantOne-site model4
ΔH [kcal/mol]1βL-wt3–16.1 ± 0.5
ΔS [cal/mol * K]2βL-wt3–26.8 ± 2.1

  • Mean values and SEM from five independent measurements.

  • ↵1Binding enthalpy (ΔH in kcal/mol).

  • ↵2Binding entropy (ΔS in cal/mol * K).

  • ↵3GlyR β-loop residues 378–426.

  • ↵4Binding isotherm fitted to a one-site interaction with gephyrin E-domain.