Table 5.

Quantification of synapses per analyzed region in sptPALM recordings of TMD-βL variants in rat spinal cord neurons

β-Loop variant	Experiment 1^¹	Experiment 2^¹	Experiment 3^¹	Average^²
TMD-βL-wt^³	53 ± 15	48 ± 28	53 ± 0	52 ± 15
TMD-βL-HI^⁴	66 ± 39	45 ± 16	100 ± 23	64 ± 31
TMD-βL-ΔCore^⁵	64 ± 20	32 ± 1	85 ± 91	61 ± 45
TMD-βL-D407P/F408G^⁶	37 ± 4	68 ± 6	48 ± 12	50 ± 15
TMD-βL-geph⁻^⁷	55 ± 13	107 ± 35	35 ± 8	72 ± 40

Average number of synapses per experiment and for all experiments.
↵ ¹Mean number of synapses per analyzed region and SD for the stated experiment (1, 2, or 3).
↵ ²mean ± SD of synapses for all three experiments.
↵ ³GlyR β-loop (residues 334–455) C-terminally fused to TMD and Dendra2.
↵ ⁴GlyR β-loop with deletion Δ378–393.
↵ ⁵GlyR β-loop with deletion Δ397–410.
↵ ⁶GlyR β-loop with substitution D407P and F408G.
↵ ⁷GlyR β-loop with substitution F398A and I400A.