Parameter | β-Loop variant | Synaptic8 Q1/Q2/Q3 | Extrasynaptic9 Q1/Q2/Q3 |
---|---|---|---|
Explored area [10−3 µm2]1 | TMD-βL-wt3 | 0.67/1.23/3.35 | 0.82/1.63/4.96 |
TMD-βL-HI4 | 0.64/1.16/2.21 | 0.75/1.39/3.53 | |
TMD-βL-βL-ΔCore5 | 1.86/4.60/11.42 | 1.51/4.63/12.77 | |
TMD-βL-D407P/F408G6 | 1.97/6.58/16.56 | 2.33/7.13/17.92 | |
TMD-βL-geph−7 | 4.27/11.50/23.03 | 3.86/11.49/24.85 | |
Deff [µm2 * s−1]2 | TMD-βL-wt3 | 0.02/0.05/0.11 | 0.04/0.07/0.26 |
TMD-βL-HI4 | 0.03/0.06/0.12 | 0.04/0.08/0.30 | |
TMD-βL-ΔCore5 | 0.05/0.12/0.30 | 0.06/0.21/0.57 | |
TMD-βL-D407P/F408G6 | 0.05/0.15/0.40 | 0.10/0.32/0.73 | |
TMD-βL-geph−7 | 0.09/0.27/0.62 | 0.15/0.44/0.86 | |
Parameter | GlyRβ variant | Synaptic (thresholded)8
Q1/Q2/Q3 | Extrasynaptic (thresholded)9
Q1/Q2/Q3 |
Explored area [10−3 µm2]1 | GlyRβ-wt10 | 0.54/0.73/1.05 | 0.60/0.91/1.76 |
GlyRβ-HI11 | 0.55/0.77/1.18 | 0.63/0.96/1.88 | |
GlyRβ-LO12 | 0.58/0.83/1.40 | 0.77/1.47/3.04 | |
GlyRβ-D407P/F408G13 | 0.60/0.86/1.52 | 0.78/1.52/3.13 | |
GlyRβ-geph−14 | 0.84/1.51/2.73 | 0.92/1.83/3.48 | |
Deff [µm2 * s−1]2 | GlyRβ-wt10 | 0.03/0.04/0.06 | 0.03/0.05/0.11 |
GlyRβ-HI11 | 0.03/0.04/0.07 | 0.03/0.05/0.11 | |
GlyRβ-LO12 | 0.03/0.04/0.09 | 0.04/0.09/0.19 | |
GlyRβ-D407P/F408G13 | 0.03/0.04/0.09 | 0.04/0.09/0.20 | |
GlyRβ-geph−14 | 0.04/0.09/0.19 | 0.05/0.11/0.22 |
Median values (Q2), 25% (Q1), and 75% (Q3) quartiles of the explored trajectory areas and Deff from >700 synaptic trajectories and >7000 extrasynaptic trajectories for each construct.
↵ 1Explored area (in 10−3 µm2).
↵ 2Deff (in µm2/s).
↵ 3GlyR β-loop (residues 334–455) C-terminally fused to TMD and Dendra2.
↵ 4GlyR β-loop with deletion Δ378–393.
↵ 5GlyR β-loop with deletion Δ397–410.
↵ 6GlyR β-loop with substitution D407P and F408G.
↵ 7GlyR β-loop with substitution F398A and I400A.
↵ 8Values for synaptic trajectories.
↵ 9Values for extrasynaptic trajectories.
↵ 10mEos4b-tagged full-length human GlyRβ subunit.
↵ 11Full-length GlyRβ with deletion Δ378–393.
↵ 12Full-length GlyRβ with deletion Δ414–426].
↵ 13Full-length GlyRβ with substitution D407P and F408G.
↵ 14Full-length GlyRβ with substitution F398A and I400A. For the full-length GlyRβ variants, a threshold of 0.02 µm2/s was applied (corresponding to the median Deff of GlyRβ-wt in fixed samples). The given Q1, Q2 and Q3 values only represent the trajectories above this threshold.