Line | Data structure | Type of test | Power |
---|---|---|---|
a | Normal distribution | Unpaired two-tailed t test | 0.0005 |
b | Normal distribution | Unpaired two-tailed t test | 0.0001 |
c | Normal distribution | Unpaired two-tailed t test | 0.0008 |
d | Normal distribution | Unpaired two-tailed t test | For KD 0.3234/for ΔH 0.6921 |
e | Normal distribution | Unpaired two-tailed t test | <0.0001 |
f | Normal distribution | Unpaired two-tailed t test | <0.0001 |
g | Normal distribution | Unpaired two-tailed t test | 0.0003 |
h | Normal distribution | Unpaired two-tailed t test | 0.001 |
i | Normal distribution | Unpaired two-tailed t test | 0.0267 |
j | Not normal distribution | One-way ANOVA/post hoc Bonferroni test | >0.05 |
k | Not normal distribution | One-way ANOVA (Kruskal–Wallis test)/post hoc Dunn’s multiple comparison test | For areas <0.001/for Deff <0.001 |
l | Not normal distribution | One-way ANOVA (Kruskal–Wallis test)/post hoc Dunn’s multiple comparison test | For areas <0.001/for Deff <0.001 |
m | Not normal distribution | One-way ANOVA (Kruskal–Wallis test)/post hoc Dunn’s multiple comparison test | For areas <0.001/for Deff <0.001 |
n | Not normal distribution | One-way ANOVA (Kruskal–Wallis test)/post hoc Dunn’s multiple comparison test | <0.001 |
o | Not normal distribution | One-way ANOVA (Kruskal–Wallis test)/post hoc Dunn’s multiple comparison test | For areas p < 0.001/for Deff p < 0.001 |
p | Not normal distribution | One-way ANOVA (Kruskal–Wallis test)/post hoc Dunn’s multiple comparison test | For areas <0.0001/for Deff <0.0001 |
ITC-derived binding parameters from a minimum of three independent measurements were compared using an unpaired two-tailed t test. Diffusion values were compared via one-way ANOVA (Kruskal–Wallis test) followed by a post hoc Dunn’s multiple comparison test. The comparison test was applied due to differences in the number of detected trajectories (n = 700–10,000 synaptic trajectories for each construct from three to five independent experiments).