Table 2.

Rise kinetics for type I and II synapses of different genotypes

	Type Ib	Type Is		Type II
Genotype	40 Hz	20 Hz	40 Hz	10 Hz	20 Hz	40 Hz
	t_1/2Rise ± SD (n, N)	t_1/2Rise ± SD (n, N)	t_1/2Rise ± SD (n, N)	t_1/2Rise ± SD (n, N)	t_1/2Rise ± SD (n, N)	t_1/2Rise ± SD (n, N)
0.1 mM Ca²⁺
WT	1.12 ± 0.26 (18,5)	1.17 ± 0.19 (99, 14)	0.68 ± 0.21 (47, 9)	0.94 ± 0.32 (68, 13)	0.67 ± 0.20 (72, 13)	0.68 ± 0.20 (24, 7)
WT + 4AP	0.66 ± 0.10 (19, 3)^***	0.39 ± 0.09 (32, 4)^***	0.34 ± 0.17 (29, 3)^***	0.76 ± 0.18 (20, 4)^**	0.48 ± 0.24 (20, 4)^**	0.49 ± 0.32 (21, 3)^**
WT + TEA	1.03 ± 0.23 (20, 4)	1.00 ± 0.22 (29, 4)^**	0.61 ± 0.13 (25, 4)	0.43 ± 0.27 (15, 5)^***	0.55 ± 0.30 (15, 5)^*	0.55 ± 0.19 (19, 4)

Sh^M	0.89 ± 0.21 (47, 7)^**	0.43 ± 0.12 (81, 9)^***	0.39 ± 0.27 (67, 9)^***	0.85 ± 0.25 (41, 6)^*	0.62 ± 0.16 (43, 6)	0.51 ± 0.22 (29, 6)^**
Sh¹²⁰	1.20 ± 0.13 (13, 3)	0.49 ± 0.21 (35, 4)^***	0.23 ± 0.14 (23, 4)^***	0.89 ± 0.19 (23, 3)^*	0.58 ± 0.14 (26, 3)^*	N.D.
eag¹	1.10 ± 0.22 (31, 6)	1.12 ± 0.29 (28, 6)	0.64 ± 0.14 (56, 6)	N.A.^†	N.A.^†	N.A.^†
eag¹ Sh¹²⁰	0.78 ± 0.23 (25, 6)^***	0.39 ± 0.23 (55, 7)^***	0.25 ± 0.13 (46, 5)^***	N.A.^†	N.A.^†	N.A.^†

para^bss1	1.10 ± 0.18 (39, 9)	0.74 ± 0.38 (65, 9)^***	0.63 ± 0.14 (29, 3)	N.A.^†	N.A.^†	N.A.^†
para^ts1	1.02 ± 0.26 (6, 3)	1.28 ± 0.19 (26, 5)^*	0.75 ± 0.20 (33, 7)	N.A.^††	N.A.^††	0.87 ± 0.31 (20, 3)
para^bss1 Sh¹²⁰	1.19 ± 0.17 (27, 4)	0.33 ± 0.06 (33, 6)^***	N.D.	0.61 ± 0.34 (11, 5)^**	0.53 ± 0.16 (22, 6) ^***	N.D.

0.5 mM Ca²⁺
WT	0.53 ± 0.19 (25, 4)^***	0.57 ± 0.12 (31, 5)^***	0.28 ± 0.06 (34, 4)^***	0.43 ± 0.17 (33, 5)^***	0.36 ± 0.21 (36, 5)^***	0.23 ± 0.13 (29, 5)^***
Sh¹²⁰	0.62 ± 0.07 (25, 3)	0.33 ± 0.11 (22, 3)⁺⁺	0.18 ± 0.05 (17, 2)⁺⁺⁺	0.52 ± 0.14 (32, 4)⁺⁺	0.27 ± 0.09 (33, 4)	0.23 ± 0.15 (27, 4)
para^bss1	0.69 ± 0.12 (26, 3)⁺⁺	0.58 ± 0.10 (48, 4)⁺⁺⁺	0.25 ± 0.06 (34, 3)	0.28 ± 0.15 (18, 4)⁺⁺⁺	0.21 ± 0.09 (19, 4)⁺⁺⁺	0.26 ± 0.14 (12, 4)

Data are presented as t_1/2Rise ± SD (n, N), where t_1/2Rise indicates half-rise time in seconds. N.A., not applicable, because of intermittent responses (†) or low-amplitude signals masked by baseline noise (††). N.D., not determined. The bolded font indicates significant difference from wild-type control. Student’s t tests were performed against WT control at the same frequency in 0.1 mM Ca²⁺ (*p < 0.05, **p < 0.01, ***p < 0.001) or in 0.5 mM Ca²⁺ (+p < 0.05, ++p < 0.01, +++p < 0.001).