Table 1.

The molarity and components of TMA-based and Cs-based internal solutions used for voltage-clamp recordings and internal solutions used for current-clamp recordings

TMA ATP-free		TMA with ATP		Cs ATP-free		Cs with ATP		Internal for current clamp ATP-free		Internal for current clamp
Molarity	Component	Molarity	Component	Molarity	Component	Molarity	Component	Molarity	Component	Molarity	Component
135 mM	TMA-OH × 5 H₂O	135 mM	TMA-OH x 5 H₂O	110 mM	Cs methane sulfonate	110 mM	Cs methane sulfonate	130 mM	K-D-gluconate	130 mM	K-D-gluconate
40 mM	HEPES	40 mM	HEPES	10 mM	HEPES	10 mM	HEPES	5 mM	EGTA	5 mM	EGTA
10 mM	EGTA	10 mM	EGTA	9 mM	EGTA	9 mM	EGTA	4 mM	NaCl	4 mM	NaCl
2 mM	MgCl₂ × 6 H₂O	14 mM	Phosphocreatine di-tris			14 mM	Phosphocreatine di-tris	0.5 mM	CaCl₂ x 2 H₂O	0.5 mM	CaCl₂ × 2 H₂O
		5 mM	Mg-ATP			5 mM	Mg-ATP	10 mM	HEPES	10 mM	HEPES
		0.3 mM	Tris-GTP			0.3 mM	Tris-GTP			2 mM	Mg-ATP
										0.5 mM	Tris-GTP

Abbreviations for different components of internal solutions are defined in Materials and Methods.