Average V50 (mean ± SEM) values for steady-state activation and inactivation with different internal solutions in CeM neurons
| TMA ATP-free | TMA with ATP | Cs ATP-free | Cs with ATP | Cs with ATP GTP-free | |
|---|---|---|---|---|---|
| V50 activation (number of cells) | –63.31 ± 0.31 (17) | –63.25 ± 0.42 (18) | –63.72 ± 0.40 (18) | –63.16 ± 0.39 (21) | –63.88 ± 0.64 (14) |
| V50 inactivation (number of cells) | –95.16 ± 0.62 (17) | –89.86 ± 0.41 (22) | –81.56 ± 1.11 (15) | –73.70 ± 0.64 (21) | –75.15 ± 0.58 (15) |
| Unpaired t test V50 inactivation | t(37) = 7.36, p < 0.001* | t(34) = 6.53, p < 0.001* t(34) = 1.61, p = 0.117ns | |||
| *TMA ATP-free vs TMA with ATP | *Cs ATP-free vs Cs with ATP nsCs with ATP GTP-free vs Cs with ATP | ||||
Note that inclusion of ATP shifted V50 for inactivation toward more depolarized potentials in both Cs-based and TMA-based solutions. In contrast, V50 for activation was minimaly affected in the same cells.