Table 1.

Summary of statistical analyses

Wild-type (WT)Nhe6-null (MUT)
Figure, statistical test, and measureBrain regionTime point, moMeanSEMnMeanSEMnStatistics
Fig. 1
Two-tailed Student’s t test
Area (cm2)
Whole brain00.3760.02190.3940.01112p = 0.42
Whole brain11.0150.012111.0300.0307p = 0.61
Whole brain21.2570.03381.1120.0426p = 0.018
Whole brain61.3260.01171.2430.0116p = 0.00025
Whole brain231.1050.01341.00680.00735p = 0.00025
Cortex00.2440.01390.24990.008312p = 0.67
Cortex10.75270.0074110.7660.0207p = 0.51
Cortex20.9080.02780.8080.0306p = 0.029
Cortex60.9490.01170.9070.0126p = 0.025
Cortex230.77230.008940.75010.00315p = 0.035
Cerebellum00.03160.002490.03230.001612p = 0.78
Cerebellum10.21880.0050110.22100.00887p = 0.83
Cerebellum20.29130.005680.2540.0116p = 0.0067
Cerebellum60.30060.003770.25900.00596p = 7.5 × 10–5
Cerebellum230.24830.007840.17600.00575p = 0.00012
Cerebellum + midbrain00.1530.01490.14410.002912p = 0.49
Cerebellum + midbrain10.26190.0062110.2640.0107p = 0.85
Cerebellum + midbrain20.34840.008380.3040.0166p = 0.021
Cerebellum + midbrain60.37760.001270.33650.00786p = 0.00015
Cerebellum + midbrain230.31240.006440.23680.00685p = 0.000096
Fig. 2
Two-tailed Student’s t test
Thickness (mm)Cortex221.2100.02931.0900.0183p = 0.025
Area (mm2)
Striatum224.6890.03034.0620.0453p = 0.00031
Hippocampus222.4550.04732.0740.0683p = 0.0098
Cerebellum227.1280.01424.5860.0894p = 4.6 × 10–5
Width (mm)
Spinal cord (Region 1)221.8900.07321.4910.0732p = 0.060
Spinal cord (Region 2)221.9870.08021.8450.0332p = 0.24
Fig. 3
Two-way ANOVA followed by Tukey’s multiple comparison test
Thickness (mm)
Cortex21.1540.01831.1260.0293p = 0.84
Cortex221.2100.02931.0900.0183p = 0.035
Area (mm2)
Striatum24.0600.07733.830.123p = 0.24
Striatum224.6890.03034.0620.0453p = 0.0021
Hippocampus22.1990.01132.1130.0623p = 0.66
Hippocampus222.4550.04732.0740.0683p = 0.0036
Cerebellum26.530.1535.910.163p = 0.034
Cerebellum227.1280.01424.5860.0894p < 0.0001
Slopes generated from linear regression
Thickness (mm)
Cortex2–220.00280.0017–0.00180.0017p = 0.10
Area (mm2)
Striatum2–220.03150.00410.01160.0066p = 0.034
Hippocampus2–220.01280.0024–0.00200.0046p = 0.021
Cerebellum2–220.03000.0097–0.06610.0085p < 0.0001
Fig. 4
Two-tailed Student’s t test
PC densityCerebellar vermis: Primary fissure52.940.1331.990.243p = 0.03
Calbindin signalCerebellar vermis: Primary fissure532.92.8415.51.74p = 0.002
PC densityCerebellar vermis: Primary fissure11–133.280.2850.770.264p < 0.001
Calbindin signalCerebellar vermis: Primary fissure11–1330.73.668.800.924p = 0.001
PC densityCerebellar vermis: Primary fissure5 and 11–13p = 0.006
Calbindin signalCerebellar vermis: Primary fissure5 and 11–13p = 0.01
Fig. 5
Two-tailed Student’s t test
PC density
Cerebellar vermis: Anterior lobe53.270.2430.740.444p = 0.006
Cerebellar vermis: Flocculonodular lobe53.490.3632.420.523p = 0.17
Cerebellar vermis: Anterior lobe11–133.220.3040.210.133p < 0.001
Cerebellar vermis: Flocculonodular lobe11–133.310.4342.780.513p = 0.46
One-way ANOVA followed by Tukey’s multiple comparison test
PC densityCerebellar vermis: Primary fissure, Anterior lobe, Flocculonodular lobe5 and 11–1311–13 mo MUT F(2,7) = 20.11
p = 0.001
Fig. 6
Two-tailed Student’s t test
PC density
Periphery54.860.4533.950.273WT vs. MUT
p = 0.16
Periphery5Periphery vs. Vermis in WT
p = 0.02
Periphery5Periphery vs. Vermis in MUT
p = 0.006
Fig. 7
Two-tailed Student’s t test
PC density
Cerebellar vermis: Primary fissure63.250.0721.020.383p = 0.02
Cerebellar vermis: Anterior lobe63.800.1220.840.583p = 0.03
Cerebellar vermis: Flocculonodular lobe63.510.4323.240.543p = 0.75
One-way ANOVA followed by Tukey’s multiple comparison test
PC densityCerebellar vermis: Primary fissure, Anterior lobe, Flocculonodular lobe6F(2,6) = 6.94
p = 0.03
Fig. 8
Two-tailed Student’s t test
PC densityCerebellar vermis: Primary fissure53.050.3530.830.454p = 0.007
Calbindin signalCerebellar vermis: Primary fissure532.12.5313.31.44p = 0.001
Fig. 10
Two-tailed Student’s t test
Microglia cell count
Cortex22159153219104p = 0.019
Striatum22398.75.63568304p = 0.0054
Hippocampus CA2274.04.43100.03.44p = 0.0048
Hippocampus DG2256.06.7367.33.94p = 0.18
Fig. 13
Two-tailed Student’s t test
Size of CD68-positive puncta in Iba1-positive cells (μm2)
Cortex2219.760.49328.02.03p = 0.015
Striatum2220.52.0329.94.03p = 0.11
Hippocampus DG227.22.2310.82.53p = 0.34
• CA, cornu Ammonis; CD68, cluster of differentiation 68; DG, dentate gyrus; Iba1, ionized calcium-binding adapter molecule 1; mo, month or months; MUT, Nhe6-null; PC, Purkinje cell; SEM, standard error of the mean; WT, wild-type.