Comparison of body weight, selected cardio-respiratory variables, and arterial blood gases between pups maintained under standard conditions (control) versus those subjected to neonatal stress (maternal separation)
| Control | Neonatal stress | ||||||
|---|---|---|---|---|---|---|---|
| Female | Male | Female | Male | Stress effect | Sex effect | Factorial interaction | |
| Body weight (g) | 41 ± 1.4 | 41 ± 1.3 | 37 ± 1.2 | 37 ± 1.4 | p = 0.003
F(1,124) = 9.408 | p = 0.49 F(1,124) = 0.469 | p = 0.96 F(1,124) = 0.002 |
| Respiratory frequency (breaths/min) | 51 ± 2.9 | 51 ± 3.5 | 47 ± 2.4 | 47 ± 2.7 | p = 0.17 F(1,120) = 1.895 | p = 0.98 F(1,120) = 0.001 | p = 0.98 F(1,120) = 0.001 |
| SpO2 (%) | 91 ± 1.0 | 90 ± 1.2 | 90 ± 1.1 | 88 ± 1.2 | p = 0.11 F(1,119) = 2.601 | p = 0.19 F(1,119) = 1.745 | p = 0.69 F(1,119) = 0.160 |
| Heart rate (beats/min) | 414 ± 15 | 418 ± 15 | 394 ± 12 | 430 ± 16 | p = 0.79 F(1,120) = 0.066 | p = 0.29 F(1,120) = 1.151 | p = 0.17 F(1,120) = 1.892 |
| Body temperature (oC) | 35.0 ± 0.0 | 35 ± 0.0 | 34.9 ± 0.0 | 35.0 ± 0.0 | p = 0.34 F(1,116) = 0.935 | p = 0.34 F(1,116) = 0.935 | p = 0.34 F(1,116) = 0.935 |
| pHa | 7.25 ± 0.03 | 7.27 ± 0.03 | 7.33 ± 0.03 | 7.36 ± 0.03 | p = 0.01
F(1,24) = 7.072 | p = 0.51 F(1,24) = 0.456 | p = 0.85 F(1,24) = 0.035 |
| PaO2 (mm Hg) | 97 ± 5.9 | 99 ± 5.6 | 102 ± 8.9 | 100 ± 4.4 | p = 0.62 F(1,25) = 0.250 | p = 0.94 F(1,25) = 0.006 | p = 0.78 F(1,25) = 0.082 |
| PaCO2 (mm Hg) | 38 ± 3.5 | 40 ± 4.1 | 32 ± 2.2 | 30 ± 3.1 | p = 0.01
F(1,25) = 7.351 | p = 0.98 F(1,25) = 0.0005 | p = 0.57 F(1,25) = 0.334 |
Measurements were performed in anesthetized pups (14-15 d old) under resting conditions. Note that blood gas measurements were obtained in a distinct series of experiments; LCR in these pups was not tested. Data are reported as mean ± SEM. A bold value indicates a significant effect of factors (ANOVA).