Table 4.

Top 5 HD markers in the striatum of Q175 altered by the TRF treatment

ComparisonQ175 vs WTAd lib vs TRF
Age2 months6 months o10 months9 months
Gene SymbolFull nameLog2 fold changeLog2 fold changep value
FosFBJ osteosarcoma oncogenensns0.0004
Htr2a*5-Hydroxytryptamine (serotonin) receptor 2Ansnsns0.0005
Hrh3Histamine receptor H3ns0.0009
Chrm4Cholinergic receptor, muscarinic 40.0012
Tacr1Tachykinin receptor 1ns0.0020
  • P value of the t test comparison with Q175 housed under ad lib is shown. Asterisk indicates HD markers changed in both the striatum and cortex. Transcripts increased by the treatment (Log2 fold change) are shown in green (↑) and those decreased by the treatment in red (↓). Transcripts without significant change (p > 0.05) are shown in gray (ns); 24% gene expressions in the striatum and 7% gene expressions in the cortex are altered by the TRF treatment. Among altered genes in striatum, >50% genes (13/24) that are shown downregulated in Q175 controls (comparison with age-matched WT controls (Lengfelder et al., 2016) are upregulated by TRF.