Statistical analysis
| Figure | Test | Sample size (n) | Test statistics | p | Power α = 0.050 |
|---|---|---|---|---|---|
| 1B | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Age F = 74.469; genotype F = 2.714; age × genotype F = 1.251 | Age p < 0.001; genotype p = 0.105; age × genotype p = 0.294 | Age α = 1.000; genotype α = 0.236; age × genotype α = 0.085 |
| 2B | Two-way ANOVA | WT: P1 = 7, P6 = 8, P14 = 10; KO: P1 = 5, P6 = 9, P14 = 10 | Age F = 234.948; genotype F = 56.277; age × genotype F = 3.471 | Age p < 0.001; genotype p < 0.001; age × genotype p = 0.040 | Age α = 1.000; genotype α = 1.000; age × genotype α = 0.460 |
| 3D | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Location F = 10.762; genotype F = 240.432; location × genotype F = 11.572 | Location p < 0.001; genotype p < 0.001; location × genotype p = 0.040 | Location α = 0.859; genotype α = 1.000; location × genotype α = 0.887 |
| 3E | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Location F = 14.142; genotype F = 39.594; location × genotype F = 5.922 | Location p < 0.001; genotype p < 0.001; location × genotype p = 0.020 | Location α = 0.958; genotype α = 1.000; location × genotype α = 0.577 |
| 3F | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Location F = 144.012; genotype F = 36.820; location × genotype F = 1.056 | Location p < 0.001; genotype p < 0.001; location × genotype p = 0.308 | Location α = 1.000; genotype α = 1.000; location × genotype α = 0.053 |
| 4B | Two-way ANOVA | WT: P1 = 7, P6 = 8, P14 = 10; KO: P1 = 5, P6 = 9, P14 = 10 | Age F = 30.240; genotype F = 48.714; age × genotype F = 2.404 | Age p < 0.001; genotype p < 0.001; age × genotype p = 0.100 | Age α = 1.000; genotype α = 1.000; age × genotype α = 0.278 |
| 5B | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Age F = 74.602; genotype F = 1.300; age × genotype F = 0.839 | Age p < 0.001; genotype p = 0.259; age × genotype p = 0.438 | Age α = 1.000; genotype α = 0.0789; age × genotype α = 0.050 |
| 5C | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Age F = 6.544; genotype F = 0.049; age × genotype F = 0.730 | Age p = 0.003; genotype p = 0.824; age × genotype p = 0.487 | Age α = 0.844; genotype α = 0.050; age × genotype α = 0.050 |
| 5E | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Age F = 188.708; genotype F = 1.683; age × genotype F = 0.860 | Age p < 0.001; genotype p = 0.200; age × genotype p = 0.428 | Age α = 1.000; genotype α = 0.120; age × genotype α = 0.050 |
| 5F | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Age F = 28.024; genotype F = 0.009; age × genotype F = 0.473 | Age p < 0.001; genotype p = 0.925; age × genotype p = 0.626 | Age α = 1.000; genotype α = 0.050; age × genotype α = 0.050 |
| 5H | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Age F = 60.344; genotype F = 0.792; age × genotype F = 0.155 | Age p < 0.001; genotype p = 0.377; age × genotype p = 0.857 | Age α = 1.000; genotype α = 0.050; age × genotype α = 0.050 |
| 5I | Two-way ANOVA | WT: P1 = 5, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 10, P14 = 16 | Age F = 35.620; genotype F = 0.724; age × genotype F = 0.620 | Age p < 0.001; genotype p = 0.398; age × genotype p = 0.541 | Age α = 1.000; genotype α = 0.050; age × genotype α = 0.050 |
| 6D | Two-way ANOVA | WT: P6 = 7, P14 = 10; KO:P6 = 13, P14 = 16 | Age F = 30.666; genotype F = 0.009; age × genotype F = 0.002 | Age p < 0.001; genotype p = 0.926; age × genotype p = 0.967 | Age α = 1.000; genotype α = 0.050; age × genotype α = 0.050 |
| 6E | Two-way ANOVA | WT: P6 = 7, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 10.846; genotype F = 0.098; age × genotype F = 0.078 | Age p = 0.002; genotype p = 0.756; age × genotype p = 0.782 | Age α = 0.883; genotype α = 0.050; age × genotype α = 0.050 |
| 6F | Two-way ANOVA | WT: P6 = 7, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 1.350; genotype F = 3.178; age × genotype F = 0.008 | Age p = 0.255; genotype p = 0.085; age × genotype p = 0.927 | Age α = 0.083; genotype α = 0.282; age × genotype α = 0.050 |
| 6G | Two-way ANOVA | WT: P6 = 7, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 15.330; genotype F = 0.195; age × genotype F = 0.094 | Age p < 0.001; genotype p = 0.661; age × genotype p = 0.760 | Age α = 0.972; genotype α = 0.050; age × genotype α = 0.050 |
| 7D | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 0.283; genotype F = 0.417; age × genotype F = 1.336 | Age p = 0.600; genotype p = 0.525; age × genotype p = 0.260 | Age α = 0.050; genotype α = 0.050; age × genotype α = 0.081 |
| 7E | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 0.804; genotype F = 66.730; age × genotype F = 0.017 | Age p = 0.379; genotype p < 0.001; age × genotype p = 0.896 | Age α = 0.050; genotype α = 1.000; age × genotype α = 0.050 |
| 7F | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO:P6 = 13, P14 = 16 | Age F = 1.785; genotype F = 4.347; age × genotype F = 0.037 | Age p = 0.194; genotype p = 0.047; age × genotype p = 0.849 | Age α = 0.128; genotype α = 0.408; age × genotype α = 0.050 |
| 7G | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 1.291; genotype F = 23.979; age × genotype F = 0.719 | Age p = 0.268; genotype p < 0.001; age × genotype p = 0.406 | Age α = 0.077; genotype α = 0.998; age × genotype α = 0.050 |
| 8D | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 16.857; genotype F = 0.210; age × genotype F = 0.169 | Age p < 0.001; genotype p = 0.649; age × genotype p = 0.683 | Age α = 0.984; genotype α = 0.050; age × genotype α = 0.050 |
| 8E | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 13.242; genotype F = 0.991; age × genotype F = 0.078 | Age p < 0.001; genotype p = 0.325; age × genotype p = 0.782 | Age α = 0.944; genotype α = 0.050; age × genotype α = 0.050 |
| 8F | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 5.388; genotype F = 40.622; age × genotype F = 0.008 | Age p < 0.001; genotype p = 0.027; age × genotype p = 0.929 | Age α = 1.000; genotype α = 0.519; age × genotype α = 0.050 |
| 8G | Two-way ANOVA | WT: P6 = 8, P14 = 10; KO: P6 = 13, P14 = 16 | Age F = 62.677; genotype F = 4.302; age × genotype F = 1.233 | Age p < 0.001; genotype p = 0.044; age × genotype p = 0.273 | Age α = 1.000; genotype α = 0.414; age × genotype α = 0.072 |
| 9D | Two-way ANOVA | WT: P1 = 7, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 14, P14 = 16 | Age F = 24.955; genotype F = 1.250; age × genotype F = 0.125 | Age p < 0.001; genotype p = 0.269; age × genotype p = 0.882 | Age α = 1.000; genotype α = 0.074; age × genotype α = 0.050 |
| 9H | Two-way ANOVA | WT: P1 = 7, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 14, P14 = 16 | Age F = 52.928; genotype F = 0.219; age × genotype F = 0.428 | Age p < 0.001; genotype p = 0.642; age × genotype p = 0.654 | Age α = 1.000; genotype α = 0.050; age × genotype α = 0.050 |
| 9L | Two-way ANOVA | WT: P1 = 7, P6 = 8, P14 = 15; KO: P1 = 5, P6 = 14, P14 = 16 | Age F = 50.641; genotype F = 0.783; age × genotype F = 1.917 | Age p < 0.001; genotype p = 0.380; age × genotype p = 0.156 | Age α = 1.000; genotype α = 0.050; age × genotype α = 0.193 |
| 10B | Two-way ANOVA | WT: P6 = 8, P14 = 13; KO: P6 = 10, P14 = 11 | Age F = 21.766; genotype F = 5.393; age × genotype F = 1.687 | Age p < 0.001; genotype p = 0.026; age × genotype p = 0.203 | Age α = 0.997; genotype α = 0.524; age × genotype α = 0.119 |
| 10D | Two-way ANOVA | WT: P6 = 8, P14 = 13; KO: P6 = 10, P14 = 11 | Age F = 19.654; genotype F = 1.462; age × genotype F = 0.256 | Age p < 0.001; genotype p = 0.234; age × genotype p = 0.616 | Age α = 0.994; genotype α = 0.095; age × genotype α = 0.050 |
| 10F | Two-way ANOVA | WT: P6 = 8, P14 = 13; KO: P6 = 10, P14 = 11 | Age F = 27.231; genotype F = 5.368; age × genotype F = 2.468 | Age p < 0.001; genotype p = 0.026; age × genotype p = 0.125 | Age α = 1.000; genotype α = 0.523; age × genotype α = 0.205 |