Table 2.

Stimulus-specific effects on single-unit NCM auditory responsiveness

Dependent variable (single-unit data)	Model	Statistical tests	F-values and degrees of freedom	p-value	Effect size (partial η²)	Post hoc results
Development (aCSF; Trial 1 only)
z-score	Phase × hemisphere × stimulus	Three-way ANOVA; Tukey’s HSD	F_{(7, 292)} = 4.682	<0.001	0.101	WN < CON1, CON2, HET, JUV CON, and JUV REV CON (p < 0.003)
Stimulus-evoked firing	Phase × hemisphere × stimulus	Three-way ANOVA; Tukey’s HSD	F_{(7, 292)} = 2.400	0.022	0.054	WN < CON1 and HET (p < 0.022)
Classification accuracy	Phase × hemisphere × stimulus	Three-way ANOVA; Tukey’s HSD	F_{(7, 262)} = 2.529	0.016	0.063	WN < JUV CON (p = 0.023)
Effect of E2 (aCSF vs. E2)
Sensory
z-score	Trial × hemisphere × stimulus	Three-way ANOVA	F_{(5, 72)} = 2.062	0.080	0.125	n/a
Stimulus-evoked firing	Trial × hemisphere × stimulus	Three-way ANOVA	F_{(5, 72)} = 1.495	0.202	0.094	n/a
Classification accuracy	Trial × hemisphere × stimulus	Three-way ANOVA	F_{(1, 54)} = 1.298	0.278	0.107	n/a
Sensorimotor
z-score
Left NCM	Trial × stimulus	Two-way ANOVA; Tukey’s HSD	F_{(7, 112)} = 3.097	0.005	0.162	WN < BOS, CON1, CON2, and HET (p < 0.038)
Right NCM	Trial × stimulus	Two-way ANOVA	F_{(5, 90)} = 2.275	0.054	0.112	n/a
Stimulus-evoked firing
Left NCM	Trial × stimulus	Two-way ANOVA	F_{(7, 112)} = 1.365	0.227	0.079	n/a
Right NCM	Trial × stimulus	Two-way ANOVA	F_{(5, 90)} = 0.558	0.732	0.030	n/a
Classification accuracy
Left NCM	Trial × stimulus	Two-way ANOVA; Tukey’s HSD	F_{(7, 112)} = 2.415	0.024	0.131	WN < JUV CON (p = 0.048)
Right NCM	Trial × stimulus	Two-way ANOVA	F_{(5, 90)} = 0.880	0.498	0.047	n/a

n/a, not applicable.