Figure | Test used | n | Defined | p value | F/t value |
---|---|---|---|---|---|

1A | Two-way ANOVA | VTA: 23, 13, 15, 14; SN-16, 9, 12, 10 | Dishes from 12 independent experiments | p < 0.0001 | F_{(1,104)} = 412.4 |

1B | Two-way ANOVA | VTA: 3, 5, 5, 3, 3; SN- 5, 4, 2, 6, 3 | Cultures from 2 independent experiments | p < 0.0001 | F_{(1,29)} = 109.5 |

1E | Unpaired t test | 4, 4 | Cells in each group | p = 0.0294 | t_{(6)} = 2.84 |

2B, VTA | One-way ANOVA | 16, 8, 6, 7, 6, 6 | VTA dishes from 8 independent experiments | p < 0.0001 | F_{(5,41)} = 15.5 |

2B, SN | One-way ANOVA | 17, 16, 7, 8, 10, 9 | SN dishes from 9 independent experiments | p < 0.0001 | F_{(5,61)} = 93.56 |

2C | Two-way ANOVA | VTA: 16, 8, 15, 6, 13; SN-11, 6, 10, 5 | Dishes from 6 independent experiments | p < 0.001 | F_{(1,69)} = 12.0 |

2E | One-way ANOVA | 14, 17, 26, 23 | Cells from 2 independent experiments | p < 0.0001 | F_{(3,76)} = 14.47 |

2G | One-way ANOVA | 40, 29, 106, 75 | Cells from 14 independent experiments | p < 0.0001 | F_{(3,246)} = 78.69 |

2H | Two-way ANOVA | VTA: 40, 27, 25, 30; SN: 70, 54, 22, 23 | Cells from 11 independent experiments | p < 0.0001 | F_{(1,283)} = 206.7 |

3A | Two-way ANOVA | VTA: 33, 6, 29, 16, 11, 19, 33, 7, 10, 19, 11, 15; SN: 34, 16, 18, 17, 11, 16, 34, 8, 19, 24, 18, 12 | Cells from 8 independent experiments | p < 0.0001 | F_{(3,412)} = 43.15 |

3B | Unpaired t test | 6, 8 | Cells from 2 independent experiments | p < 0.05 | t_{(12)} = 3.52 |

3D | Two-way ANOVA | VTA: 71, 27, 38, 11; SN: 57, 24, 35, 23 | Cells from 7 independent experiments | p < 0.0001 | F_{(1,278)} = 73.09 |

4B | Two-way ANOVA | VTA: 23, 14, 24, 24; SN: 51, 36, 22, 9 | Cells from 4 independent experiments | p < 0.0001 | F_{(1,195)} = 101.1 |

4C | One-way ANOVA | VTA: 46, 23, 51, 35; SN: 68, 11, 100, 59, 92, 48, 29, 41, 43 | Cells from 14 independent experiments | p < 0.0001 | F_{(12,633)} = 60.75 |

4D | One-way ANOVA | VTA: 67, 37; SN: 68, 48, 37 | Cells from 3 independent experiments | p < 0.0001 | F_{(4,192)} = 9.16 |

5C | One-way ANOVA | VTA: 36, 57; SN: 75, 46, 54, 20, 42, 28, 28 | Cells from 8 independent experiments | p < 0.0001 | F_{(8,377)} = 52.62 |

5E | One-way ANOVA | VTA: 24, 28; SN: 18, 26, 29 | Cells from 3 independent experiments | p < 0.0001 | F_{(4,120)} = 9.32 |

6A | One-way ANOVA | VTA: 9, 10, 10, 9; SN: 25, 28, 14, 9, 10, 10 | Cells from 4 independent experiments | p < 0.0001 | F_{(9,124)} = 40.1 |

6B | One-way ANOVA | VTA: 64, 42, 25; SN: 54, 38, 20 | Cells from 7 independent experiments | p < 0.0001 | F_{(5,127)} = 31.04 |

6C | One-way ANOVA | 80, 26, 15, 21, 78, 33, 29, 43, 22, 21 | Cells from 14 independent experiments | p < 0.0001 | F_{(9,358)} = 20.75 |

6D | Two-way ANOVA | 16, 20, 18, 16 | Cells from 2 independent experiments | p = 0.4593 | F_{(1,66)} = 0.55 |

7A | One-way ANOVA | 24, 15, 22, 12 | Cells from 5 independent experiments | p < 0.0001 | F_{(3,246)} = 78.69 |

7B | One-way ANOVA | 24, 44, 25, 17, 38, 27, 33, 9, 19 | Cells from 15 independent experiments | p < 0.0001 | F_{(8,227)} = 13.69 |

7C | One-way ANOVA | 14, 17, 23 | Cells from 3 independent experiments | p < 0.0001 | F_{(2,51)} = 19.0 |

7D, VTA | One-way ANOVA | 16, 10, 5, 3 | Dishes from 5 independent experiments | p < 0.0001 | F_{(3,30)} = 12.28 |

7D, SN | One-way ANOVA | 17, 23, 8, 8, 6 | Dishes from 7 independent experiments | p < 0.0001 | F_{(4,57)} = 139.2 |

7F | One-way ANOVA | VTA: 26, 23, 22; SN: 16, 23, 22 | Cells from 2 independent experiments | p < 0.05 | F_{(5,126)} = 13.89 |

7G | One-way ANOVA | 22, 28, 25, 10, 6, 6, 10 | Dishes from 13 independent experiments | p < 0.0001 | F_{(6,99)} = 157.6 |

7H | One-way ANOVA | VTA: 12, 12, 13; SN: 7, 7, 8 | Dishes from 4 independent experiments | p < 0.0001 | F_{(5,53)} = 95.53 |

8A | One-way ANOVA | WT: 16, 12, 8; KO: 14, 11, 6 | Dishes from 3 independent experiments | p < 0.0001 | F_{(5,58)} = 80.15 |

8B | One-way ANOVA | 23, 15, 6, 6 | Dishes from 2 independent experiments | p < 0.01 | F_{(3,46)} = 14.12 |

8C | Two-way ANOVA | WT: 18, 20, 18; KO: 20, 16, 24 | Cells from 2 independent experiments | p = 0.4988 | F_{(1,110)} = 0.46 |

8D | One-way ANOVA | 104, 124, 41, 83 | Cells from 5 independent experiments | p < 0.0001 | F_{(3,348)} = 59.46 |

8E | One-way ANOVA | 18, 26, 18, 19 | Cells from 3 independent experiments | p < 0.0001 | F_{(3,77)} = 8.15 |

8F | Two-way ANOVA | WT: 7, 10, 15; KO: 7, 10, 10 | Cells from 3 independent experiments | p < 0.0001 | F_{(1,53)} = 20.67 |

9B | One-way ANOVA | 97, 104, 127, 83 | Cells from 2 independent experiments | p < 0.0001 | F_{(3,407)} = 224.8 |

9C | One-way ANOVA | 97, 104, 38, 41 | Cells from 3 independent experiments | p < 0.0001 | F_{(3,276)} = 32.04 |

9D | One-way ANOVA | Ctrl: 75, 54, 20; mKate: 40, 42; hWT: 50, 52, 54 | Cells from 4 independent experiments | p < 0.0001 | F_{(7,379)} = 63.87 |

9E | One-way ANOVA | Ctrl: 36, 57, 61; mKate: 21, 21; hWT: 64, 64, 33 | Cells from 4 independent experiments | p = 0.7016 | F_{(7,349)} = 0.67 |

Extended Data figures | |||||

1-1C | Two-way ANOVA | VTA:13; SN: 11 | Cells in 2 independent experiments | p < 0.01 | F_{(1,392)} = 9.85 |

1-1D | Mann-Whitney | 13, 11 | Cells in 2 independent experiments | p = 0.506 | U = 60.5 |

1-1E | Two-way ANOVA | VTA: 13; SN: 11 | Cells in 2 independent experiments | p = 0.053 | F_{(1,175)} = 3.79 |

1-1F | Mann-Whitney | 15, 11 | Cells in 2 independent experiments | p = 0.643 | U = 80.0 |

1-1G | Mann-Whitney | 14, 12 | Cells in 2 independent experiments | p = 0.699 | U = 76.0 |

1-1H | Mann-Whitney | 14, 12 | Cells in 2 independent experiments | p = 0.625 | U = 74.0 |

1-2A | Two-way ANOVA | WT: 16, 12, 10; THGFP: 6, 5, 5, 5; THmito: 6, 5, 5, 5; DATGCaMP: 6, 5, 5, 5 | Cells from 6 independent experiments | p = 0.4232 | F_{(3,74)} = 0.95 |

1-2B | Two-way ANOVA | WT: 23, 15, 14; THGFP: 6, 5, 5, 5; THmito: 6, 5, 6, 5; DATGCaMP: 6, 5, 5, 5 | Cells from 6 independent experiments | p = 0.9491 | F_{(3,89)} = 0.12 |

4-1 | Unpaired t test | 33, 32 | Cells from 2 independent experiments | p = 0.8678 | t_{(63)} = 0.1675 |

5-1B | Two-way ANOVA | VTA: 18, 8, 26; SN: 34, 7, 37; effect of the drug | Cells from 4 independent experiments | p < 0.002 | F_{(2,131)} = 6.61 |

5-1B | Two-way ANOVA | VTA: 18, 8, 26; SN: 34, 7, 37; effect between the cell types | Cells from 4 independent experiments | p = 0.1429 | F_{(1,131)} = 2.17 |

5-1C | One-way ANOVA | 36, 24, 57, 61 | Cells from 7 independent experiments | p = 0.1601 | F_{(3,174)} = 1.74 |

5-2 | One-way ANOVA | 68, 100, 59, 28, 29, 14 | Cells from 7 independent experiments | p < 0.0001 | F_{(5,292)} = 37.02 |

6-1 | One-way ANOVA | 33, 25, 9, 27, 30 | Cells from 6 independent experiments | p < 0.0001 | F_{(4,119)} = 9.47 |

6-2 | One-way ANOVA | 74, 27, 75, 33, 24, 40 | Cells from 8 independent experiments | p < 0.0001 | F_{(5,267)} = 26.46 |