Table 1

Details of statistical analyses between all experimental groups presented in the paper.

FigureTest usednDefinedp valueF/t value
1ATwo-way ANOVAVTA: 23, 13, 15, 14; SN-16, 9, 12, 10Dishes from 12 independent experimentsp < 0.0001F(1,104) = 412.4
1BTwo-way ANOVAVTA: 3, 5, 5, 3, 3; SN- 5, 4, 2, 6, 3Cultures from 2 independent experimentsp < 0.0001F(1,29) = 109.5
1EUnpaired t test4, 4Cells in each groupp = 0.0294t(6) = 2.84
2B, VTAOne-way ANOVA16, 8, 6, 7, 6, 6VTA dishes from 8 independent experimentsp < 0.0001F(5,41) = 15.5
2B, SNOne-way ANOVA17, 16, 7, 8, 10, 9SN dishes from 9 independent experimentsp < 0.0001F(5,61) = 93.56
2CTwo-way ANOVAVTA: 16, 8, 15, 6, 13; SN-11, 6, 10, 5Dishes from 6 independent experimentsp < 0.001F(1,69) = 12.0
2EOne-way ANOVA14, 17, 26, 23Cells from 2 independent experimentsp < 0.0001F(3,76) = 14.47
2GOne-way ANOVA40, 29, 106, 75Cells from 14 independent experimentsp < 0.0001F(3,246) = 78.69
2HTwo-way ANOVAVTA: 40, 27, 25, 30; SN: 70, 54, 22, 23Cells from 11 independent experimentsp < 0.0001F(1,283) = 206.7
3ATwo-way ANOVAVTA: 33, 6, 29, 16, 11, 19, 33, 7, 10, 19, 11, 15; SN: 34, 16, 18, 17, 11, 16, 34, 8, 19, 24, 18, 12Cells from 8 independent experimentsp < 0.0001F(3,412) = 43.15
3BUnpaired t test6, 8Cells from 2 independent experimentsp < 0.05t(12) = 3.52
3DTwo-way ANOVAVTA: 71, 27, 38, 11; SN: 57, 24, 35, 23Cells from 7 independent experimentsp < 0.0001F(1,278) = 73.09
4BTwo-way ANOVAVTA: 23, 14, 24, 24; SN: 51, 36, 22, 9Cells from 4 independent experimentsp < 0.0001F(1,195) = 101.1
4COne-way ANOVAVTA: 46, 23, 51, 35; SN: 68, 11, 100, 59, 92, 48, 29, 41, 43Cells from 14 independent experimentsp < 0.0001F(12,633) = 60.75
4DOne-way ANOVAVTA: 67, 37; SN: 68, 48, 37Cells from 3 independent experimentsp < 0.0001F(4,192) = 9.16
5COne-way ANOVAVTA: 36, 57; SN: 75, 46, 54, 20, 42, 28, 28Cells from 8 independent experimentsp < 0.0001F(8,377) = 52.62
5EOne-way ANOVAVTA: 24, 28; SN: 18, 26, 29Cells from 3 independent experimentsp < 0.0001F(4,120) = 9.32
6AOne-way ANOVAVTA: 9, 10, 10, 9; SN: 25, 28, 14, 9, 10, 10Cells from 4 independent experimentsp < 0.0001F(9,124) = 40.1
6BOne-way ANOVAVTA: 64, 42, 25; SN: 54, 38, 20Cells from 7 independent experimentsp < 0.0001F(5,127) = 31.04
6COne-way ANOVA80, 26, 15, 21, 78, 33, 29, 43, 22, 21Cells from 14 independent experimentsp < 0.0001F(9,358) = 20.75
6DTwo-way ANOVA16, 20, 18, 16Cells from 2 independent experimentsp = 0.4593F(1,66) = 0.55
7AOne-way ANOVA24, 15, 22, 12Cells from 5 independent experimentsp < 0.0001F(3,246) = 78.69
7BOne-way ANOVA24, 44, 25, 17, 38, 27, 33, 9, 19Cells from 15 independent experimentsp < 0.0001F(8,227) = 13.69
7COne-way ANOVA14, 17, 23Cells from 3 independent experimentsp < 0.0001F(2,51) = 19.0
7D, VTAOne-way ANOVA16, 10, 5, 3Dishes from 5 independent experimentsp < 0.0001F(3,30) = 12.28
7D, SNOne-way ANOVA17, 23, 8, 8, 6Dishes from 7 independent experimentsp < 0.0001F(4,57) = 139.2
7FOne-way ANOVAVTA: 26, 23, 22; SN: 16, 23, 22Cells from 2 independent experimentsp < 0.05F(5,126) = 13.89
7GOne-way ANOVA22, 28, 25, 10, 6, 6, 10Dishes from 13 independent experimentsp < 0.0001F(6,99) = 157.6
7HOne-way ANOVAVTA: 12, 12, 13; SN: 7, 7, 8Dishes from 4 independent experimentsp < 0.0001F(5,53) = 95.53
8AOne-way ANOVAWT: 16, 12, 8; KO: 14, 11, 6Dishes from 3 independent experimentsp < 0.0001F(5,58) = 80.15
8BOne-way ANOVA23, 15, 6, 6Dishes from 2 independent experimentsp < 0.01F(3,46) = 14.12
8CTwo-way ANOVAWT: 18, 20, 18; KO: 20, 16, 24Cells from 2 independent experimentsp = 0.4988F(1,110) = 0.46
8DOne-way ANOVA104, 124, 41, 83Cells from 5 independent experimentsp < 0.0001F(3,348) = 59.46
8EOne-way ANOVA18, 26, 18, 19Cells from 3 independent experimentsp < 0.0001F(3,77) = 8.15
8FTwo-way ANOVAWT: 7, 10, 15; KO: 7, 10, 10Cells from 3 independent experimentsp < 0.0001F(1,53) = 20.67
9BOne-way ANOVA97, 104, 127, 83Cells from 2 independent experimentsp < 0.0001F(3,407) = 224.8
9COne-way ANOVA97, 104, 38, 41Cells from 3 independent experimentsp < 0.0001F(3,276) = 32.04
9DOne-way ANOVACtrl: 75, 54, 20; mKate: 40, 42; hWT: 50, 52, 54Cells from 4 independent experimentsp < 0.0001F(7,379) = 63.87
9EOne-way ANOVACtrl: 36, 57, 61; mKate: 21, 21; hWT: 64, 64, 33Cells from 4 independent experimentsp = 0.7016F(7,349) = 0.67
Extended Data figures
1-1CTwo-way ANOVAVTA:13; SN: 11Cells in 2 independent experimentsp < 0.01F(1,392) = 9.85
1-1DMann-Whitney13, 11Cells in 2 independent experimentsp = 0.506U = 60.5
1-1ETwo-way ANOVAVTA: 13; SN: 11Cells in 2 independent experimentsp = 0.053F(1,175) = 3.79
1-1FMann-Whitney15, 11Cells in 2 independent experimentsp = 0.643U = 80.0
1-1GMann-Whitney14, 12Cells in 2 independent experimentsp = 0.699U = 76.0
1-1HMann-Whitney14, 12Cells in 2 independent experimentsp = 0.625U = 74.0
1-2ATwo-way ANOVAWT: 16, 12, 10; THGFP: 6, 5, 5, 5; THmito: 6, 5, 5, 5; DATGCaMP: 6, 5, 5, 5Cells from 6 independent experimentsp = 0.4232F(3,74) = 0.95
1-2BTwo-way ANOVAWT: 23, 15, 14; THGFP: 6, 5, 5, 5; THmito: 6, 5, 6, 5; DATGCaMP: 6, 5, 5, 5Cells from 6 independent experimentsp = 0.9491F(3,89) = 0.12
4-1Unpaired t test33, 32Cells from 2 independent experimentsp = 0.8678t(63) = 0.1675
5-1BTwo-way ANOVAVTA: 18, 8, 26; SN: 34, 7, 37; effect of the drugCells from 4 independent experimentsp < 0.002F(2,131) = 6.61
5-1BTwo-way ANOVAVTA: 18, 8, 26; SN: 34, 7, 37; effect between the cell typesCells from 4 independent experimentsp = 0.1429F(1,131) = 2.17
5-1COne-way ANOVA36, 24, 57, 61Cells from 7 independent experimentsp = 0.1601F(3,174) = 1.74
5-2One-way ANOVA68, 100, 59, 28, 29, 14Cells from 7 independent experimentsp < 0.0001F(5,292) = 37.02
6-1One-way ANOVA33, 25, 9, 27, 30Cells from 6 independent experimentsp < 0.0001F(4,119) = 9.47
6-2One-way ANOVA74, 27, 75, 33, 24, 40Cells from 8 independent experimentsp < 0.0001F(5,267) = 26.46