Body weight, basal CORT concentration, and GR expression (fold induction, standardized to the noMD group at PND26) in the dorsal hippocampus, ventromedial prefrontal cortex (vmPFC), and dorsal striatum of male rats at PND26, PND29, and PND90
| noMD | MD | Condition | |||||
|---|---|---|---|---|---|---|---|
| PND26 | |||||||
| Body weight (BW in g) | 59 ± 1 | 52 ± 1 | t(78) = 6; p < 0.0001 | ||||
| Basal CORT (ng/ml) | 57 ± 20 | 178 ± 45 | t(22) = 5.7; p = 0.025 | ||||
| GR hippocampus | 1.0 ± 0.3 | 1.0 ± 0.2 | t(15) = 0.23; p = 0.140 | ||||
| GR vmPFC | 1.0 ± 0.2 | 2.4 ± 0.6 | t(13) = −2.2; p = 0.049 | ||||
| GR striatum | 1.0 ± 0.2 | 1.8 ± 0.6 | t(8) = 0.2; p = 0.749 | ||||
| PND29 | noMD VEH | noMD MIF | MD VEH | MD MIF | Condition × treatment | Condition (noMD vs MD) | Treatment (VEH vs MIF) |
| Body weight (BW in g) | 75 ± 2 | 72 ± 1 | 65 ± 1 | 64 ± 2 | F(1,28) = 0.4; p = 0.532 | F(1,28) = 47; p < 0.0001 | F(1,28) = 1.9; p = 0.180 |
| Basal CORT (ng/ml) | 228 ± 60 | 122 ± 45 | 68 ± 34 | 115 ± 65 | F(1,20) = 2; p = 0.172 | F(1,20) = 2.4;p = 0.140 | F(1,20) = 0.3; p = 0.593 |
| GR hippocampus | 1.2 ± 0.2 | 0.8 ± 0.1 | 1.1 ± 0.3 | 1.2 ± 0.2 | F(1,27) = 1.5; p = 0.226 | F(1,27) = 0.35 p = 0.560 | F(1,27) = 0.41; p = 0.525 |
| GR vmPFC | 1.2 ± 0.3 | 1.9± 0.6 | 1.4 ± 0.4 | 1.1 ± 0.3 | F(1,24) = 1; p = 0.322 | F(1,24) = 0.4; p = 0.524 | F(1,24) = 3.7; p = 0.545 |
| GR striatum | 1.6 ± 0.7 | 2.2 ± 0.8 | 1.3 ± 0.3 | 1.4 ± 0.5 | F(1,20) = 0.2; p = 0.692 | F(1,20) = 0.8; p = 0.370 | F(1,20) = 0.3; p = 0.590 |
| PND90 | noMD VEH | noMD MIF | MD VEH | MD MIF | Condition × treatment | Condition (noMD vs MD) | Treatment (VEH vs MIF) |
| Body weight (BW in g) | 329 ± 6 | 334 ± 6 | 333 ± 5 | 331 ± 8 | F(1,29) = 1.3; p = 0.262 | F(1,29) = 0.7; p = 0.415 | F(1,29) = 0.8; p = 0.368 |
| Basal CORT (ng/ml) | 191 ± 60 | 206 ± 56 | 104 ± 21 | 118 ± 37 | F(1,24) = 0.0; p = 0.991 | F(1,24) = 3.6; p = 0.071 | F(1,24) = 0.10; p = 0.753 |
| GR hippocampus | 1.4± 0.4 | 0.9 ± 0.3 | 1.4 ± 0.4 | 0.8 ± 0.2 | F(1,26) = 0.3; p = 0.871 | F(1,26) = 0.4; p = 0.834 | F(1,26) = 3.2; p = 0.9 |
| GR vmPFC | 2.2 ± 0.9 | 1.2 ± 0.3 | 1.2 ± 0.3 | 1.3 ± 0.5 | F(1,24) = 0.9; p = 0.350 | F(1,24) = 0.6; p = 0.430 | F(1,24) = 0.8; p = 0.375 |
| GR striatum | 1.1 ± 0.8 | 1.8 ± 1 | 1.3 ± 0.5 | 0.6 ± 0.2 | F(1,19) = 1.1; p = 0.300 | F(1,19) = 0.5; p = 0.505 | F(1,19) = 0.0; p = 0.994 |
All animals had been subjected to 24-h MD at PND3 or control treatment. Half of the animals were treated with MIF through oral gavage twice daily on PND26–PND28; the other half received VEH. Data represent mean ± SEM; all groups n = 6–8 animals, except for body weight at PND26, which was determined for all animals.