Three to four weeks | Six to eight weeks | |||||
---|---|---|---|---|---|---|
WT | KO | Statistical value | WT | KO | Statistical value | |
Input resistance (MΩ) | 91.51 ± 4.96 | 104.33 ± 13.07 | t_{(23)} = 1.125 p = 0.2721 | 75.49 ± 6.19 | 82.32 ± 5.57 | t_{(22)} = 0.8119 p = 0.4255 |
V_spike peak (mV) | 46.41 ± 1.17 | 42.93 ± 2.65 | t_{(23)} = 1.407 p = 0.1727 | 48.54 ± 1.65 | 46.75 ± 1.34 | t_{(22)} = 0.8498 p = 0.4046 |
V_trough (mV) | −47.87 ± 0.44 | −49.13 ± 0.94 | t_{(23)} = 1.388 p = 0.1785 | −48.08 ± 0.59 | −48.46 ± 0.62 | t_{(22)} = 0.4247 p = 0.6752 |
Spike height (mV) | 94.28 ± 1.41 | 92.06 ± 3.15 | t_{(23)} = 0.7528 p = 0.4592 | 96.63 ± 1.97 | 95.21 ± 1.62 | p = 0.5036* |
Spike width (ms) | 1.18 ± 0.04 | 1.22 ± 0.05 | t_{(23)} = 0.5595 p = 0.5812 | 1.05 ± 0.06 | 0.92 ± 0.03 | t_{(22)} = 2.069 p = 0.0505 |
V_threshold (mV) | −37.25 ± 0.56 | −38.67 ± 0.90 | t_{(23)} = 1.401 p = 0.1747 | −38.20 ± 1.02 | −39.14 ± 0.58 | t_{(22)} = 0.8592 p = 0.3995 |
Upstroke (mV/ms) | 265.83 ± 8.82 | 250.42 ± 18.31 | t_{(23)} = 0.8626 p = 0.3973 | 349.53 ± 17.73 | 358.53 ± 12.56 | p = 0.9431* |
Downstroke (mV/ms) | −68.02 ± 2.31 | −65.86 ± 3.44 | t_{(23)} = 0.5238 p = 0.6054 | −79.90 ± 4.59 | −91.96 ± 3.79 | t_{(22)} = 2.033 p = 0.0543 |
Upstroke/downstroke ratio | −3.93 ± 0.11 | −3.79 ± 0.18 | t_{(23)} = 0.7090 p = 0.4854 | −4.45 ± 0.26 | −3.93 ± 0.11 | t_{(22)} = 2.088p = 0.0486 |
RMP (mV) | −68.40 ± 0.81 | −67.01 ± 1.71 | p = 0.6659* | −70.27 ± 1.10 | −69.72 ± 0.83 | t_{(22)} = 0.4048 p = 0.6895 |
First ISI (ms) | 6.95 ± 0.26 | 7.86 ± 1.47 | t_{(23)} = 0.8638 p = 0.3966 | 6.41 ± 0.46 | 5.26 ± 0.58 | p = 0.0220* |
Mean ISI (ms) | 26.99 ± 0.76 | 28.63 ± 2.24 | p = 0.7504* | 29.41 ± 1.43 | 26.41 ± 1.44 | t_{(22)} = 1.436 p = 0.1650 |
Adaptation index | 0.02 ± 0.001 | 0.02 ± 0.001 | t_{(23)} = 0.6344 p = 0.5321 | 0.02 ± 0.004 | 0.03 ± 0.002 | p = 0.7879* |
Slope of current-firing curve | 7.02 ± 0.23 | 6.41 ± 0.60 | p = 0.0883* | 6.35 ± 0.28 | 7.19 ± 0.47 | p = 0.1583* |
Spike features were obtained from the first action potential evoked by the step current which was just above the threshold. ISIs were obtained from the response evoked by the step current which was 360 pA above the threshold. Two-tailed unpaired t test or M–W test (*) was used for statistical analyses. Bold text indicates statistical significance, p < 0.05.