Data structure | Type of test | Test values and power | |
---|---|---|---|

Fig. 2A | N/A | Fisher’s exact test | p = 0.0386 |

Fig. 2B, two months | All but one group normally distributed (D7 WT) | Repeated measures two-way ANOVA with Bonferroni post hoc tests | Days in cage: F_{(2,56)} = 20.11, p < 0.0001; genotype: F_{(1,28)} = 0.0007, p = 0.9798; interaction: F_{(2,56)} = 0.0260, p = 0.9743 |

Fig. 2B, four months | All groups normally distributed | Repeated measures two-way ANOVA with Bonferroni post hoc tests | Days in cage: F_{(2,56)} = 6.050, p = 0.0042; genotype: F_{(1,28)} = 0.08113, p = 0.7779; interaction: F_{(2,56)} = 0.4340, p = 0.6501 |

Fig. 2B, six months | All groups normally distributed | Repeated measures two-way ANOVA with Bonferroni post hoc tests | Days in cage: F_{(2,56)} = 3.936, p = 0.0252; genotype: F_{(1,28)} = 0.2267, p = 0.6376; interaction: F_{(2,56)} = 0.3738, p = 0.6898 |

Fig. 2C (log transform) | All but one group (six-month YAC128) normally distributed, equal variances | Two-way ANOVA | Age: F_{(2,84)} = 4.803, p = 0.0106; genotype: F_{(1,84)} = 0.1089, p = 0.7422; interaction: F_{(2,84)} = 0.5332, p = 0.5887 |

Fig. 2D, two months | Normal distribution, equal variances | Student’s t test | t_{(28)} = 0.7433, p = 0.4635 |

Fig. 2D, four months | Non-normal distribution | Mann-Whitney test | U = 94, p = 0.4659 |

Fig. 2D, six months | Non-normal distribution | Mann-Whitney test | U = 107, p = 0.9665 |

Fig. 2D, WT | Non-normal distribution | Kruskal-Wallis Test with Dunn’s post hoc tests | H = 15.22, p = 0.0005 |

Fig. 2D, YAC128 | Non-normal distribution | Kruskal-Wallis Test with Dunn’s post hoc tests | H = 13.50, p = 0.0012 |

Fig. 3B | Groups normally distributed, equal variances | Two-way ANOVA | Age: F_{(2,84)} = 2.945, p = 0.0580; genotype: F_{(1,84)} = 4.772, p = 0.0317; interaction: F_{(2,84)} = 0.2492, p = 0.7800 |

Fig. 3C | Groups normally distributed | Repeated measures two-way ANOVA | Hour of day: F_{(23,644)} = 86.51, p < 0.0001; genotype: F_{(1,28)} = -0.3218, p > 0.9999; interaction: F_{(23,644)} = 0.7632, p = 0.7788 |

Fig. 3D | Groups normally distributed | Repeated measures two-way ANOVA with Bonferroni post hoc tests | Hour of day: F_{(23,598)} = 56.36, p < 0.0001; genotype: F_{(1,26)} = 0.0, p > 0.9999; interaction: F_{(23,598)} = 2.296, p = 0.0006 |

Fig. 3E | Groups normally distributed | Repeated measures two-way ANOVA with Bonferroni post hoc tests | Hour of day: F_{(23,644)} = 43.87, p < 0.0001; genotype: F_{(1,28)} = 0.8750, p = 0.3576; interaction: F_{(23,644)} = 1.911, p = 0.0066 |

Fig. 4A | Groups normally distributed | Repeated measures two-way ANOVA with Bonferroni post hoc tests | Trial number: F_{(20,500)} = 70.42, p < 0.0001; genotype: F_{(1, 25)} = 6.367, p = 0.0184; interaction: F_{(20,500)} = 5.321, p < 0.0001 |

Fig. 4B | Groups normally distributed | Repeated measures two-way ANOVA | Trial number: F_{(20,460)} = 115.8, p < 0.0001; genotype: F_{(1,23)} = 0.02924, p = 0.8657; interaction: F_{(20,460)} = 0.6740, p = 0.8528 |

Fig. 4C | Groups normally distributed | Repeated measures two-way ANOVA | Trial number: F_{(20,520)} = 115.7, p < 0.0001; genotype: F_{(1,26)} = 0.2737, p = 0.6053; interaction: F_{(20,520)} = 1.336, p = 0.1497 |

Fig. 4D | N/A | Fisher’s exact test | p = 0.7292 |

Fig. 4E | Groups normally distributed, equal variances | Two-way ANOVA | Age: F_{(2,74)} = 2.753, p = 0.0703; genotype: F_{(1,74)} = 2.002, p = 0.1613; interaction: F_{(2,74)} = 1.504, p = 0.2290 |

Fig. 5E | Groups normally distributed, equal variances | Two-way ANOVA with Bonferroni post hoc tests | Age: F_{(2,64)} = 3.193, p = 0.0477; genotype: F_{(1,64)} = 2.798, p = 0.0993; interaction: F_{(2,64)} = 2.522, p = 0.0883 |

Fig. 5F | All but one group (two-month YAC128) normally distributed, equal variances | Two-way ANOVA with Bonferroni post hoc tests | Age: F_{(2,64)} = 0.8329, p = 0.4395; genotype: F_{(1,64)} = 3.837, p = 0.0545; interaction: F_{(2,64)} = 6.309, p = 0.0032 |

Fig. 5G | Groups normally distributed, equal variances | Two-way ANOVA with Bonferroni post hoc tests | Age: F_{(2,64)} = 1.188, p = 0.3113; genotype: F_{(1,64)} = 2.193, p = 0.1435; interaction: F_{(2,64)} = 3.381, p = 0.0402 |