Table 1.

Antibodies used in this study

Antibody nameSpecies/isotype/immunogenManufacturer informationConcentration used
AMIGO-1, anti-AMIGO-1 rabbit pAbRaised against aa 394–492 of mouse AMIGO-1 (cytoplasmic C-terminus).Trimmer Lab. Rabbit 28330 RRID:AB_25715151:400 dilution of affinity purified pAb, concentration unknown
L98/12, anti-AMIGO-1 mouse IgG1 mAbRaised against aa 28–370 of mouse AMIGO-1 (extracellular N-terminus).Trimmer lab.
RRID:AB_2571516
1:3 dilution of tissue culture supernatant, concentration unknown
K89/34, anti-Kv2.1 mouse IgG1 mAbRaised against aa 837–853 of rat Kv2.1.Trimmer lab. NeuroMab catalog 73-014
RRID:AB_10672253
5 µg/ml purified mAb
L61C/30, anti-Kv2.1 mouse IgG1 mAbRaised against aa 595–616 of rat Kv2.1.Trimmer lab. RRID:AB_25321001:5 dilution of tissue culture supernatant, concentration unknown
N372B/1, anti-Kv2.2 mouse IgG1 mAbRaised against aa 717–907 of rat Kv2.2. Binds within aa 764–907. Species reactivity with mouse, rat, ferret, macaque and humanNeuroMab catalog 73–369, RRID:AB_23158691:3 dilution of tissue culture supernatant, concentration unknown
N372B/60, anti-Kv2.2 mouse IgG2b mAbRaised against aa 717–907 of rat Kv2.2. Binds within aa 764–907. Species reactivity with mouse and ratNeuroMab catalog 73–360, RRID:AB_23158671:10 dilution of tissue culture supernatant, concentration unknown
N372C/51, anti-Kv2.2 mouse IgG1 mAbRaised against aa 717–907 of rat Kv2.2. Binds within aa 717–763 Species reactivity with mouse and ratNeuroMab catalog 73–358, RRID:AB_23158651:2 dilution of tissue culture supernatant, concentration unknown
N133/21, anti-RGS14 mouse IgG2a mAbRaised against aa 1–544 of rat RGS14.NeuroMab catalog 73–170, RRID:AB_106980261:10 dilution of tissue culture supernatant, concentration unknown
  • Details of the polyclonal (pAb) and monoclonal (mAb) Abs used in this study.