Table 3.

List of anti-PGRN primary antibodies used in this study

VendorCatalog number/(RRID)SpeciesClonalityImmunogenAntibody stockImmunoblot dilutionICC dilution
R&D SystemsAF2420/(2114489)GoatPolyaa 18-5931 µg/µl1:500-1:10001:500
R&D SystemsMAB2420/(2114488)MouseMonoaa 18-5931 µg/µl1:500-1:10001:300
Sigma (Atlas)HPA008763/(1234492)RabbitPolyaa 128-2710.1 µg/µl1:500-1:10001:250
Life Technologies40-3400/(2533461)RabbitPolyC-terminal protein0.25 µg/µl1:500-1:10001:50
Novus/SDIX26320002/(2114484)RabbitPolyaa 289-4260.5 µg/µl1:5001:250
Abcam/Epitomicsab108608/EPR3781/(10888818)RabbitMonoSynthetic protein0.5 µg/µl1:500N/A
ENZOALX-804-737-C100/(2052325)MouseMonoHuman PGRN1 µg/µl1:1000N/A
Santa Cruz(C-11) sc-377036/(Not listed)MouseMonoaa 21-3200.2 µg/µl1:2501:50
GenScriptLinker-3 (custom)/(n/a)RabbitPolyaa 187-200 LAKKLPAQRTNRAVC0.18 µg/µl1:10,000N/A
Custom4C1 (custom)/(n/a)MouseMonoHuman C-TAP PGRNUnknown1:50N/A
LS BIOLS-C154960/(Not listed)GoatPolyaa 248-2590.5 µg/µl1:5001:50
AdipogenAG-25A-0090-C100/(249045)RabbitPolyHuman GRN C1 µg/µl1:10001:250
  • aa = amino acid, ICC = immunocytochemistry