Synaptic and intrinsic properties of CA1 pyramidal cells at 6-8 d after ablation
AAV-DTR + DT | AAV-DTR alone | DT alone | Statistics | ||
---|---|---|---|---|---|
Ave ± SD | Ave ± SD | Ave ± SD | One-way ANOVA | ||
mEPSC | IEI (ms) | 1806.8 ± 805.9 | 2465.6 ± 1091.5 | 2199.4 ± 1057.2 | F(2,44) = 1.75, p = 0.19 |
Amplitude (pA) | 31.7 ± 4.1 | 32.8 ± 5.9 | 31.0 ± 7.4 | F(2,44) = 0.36, p = 0.70 | |
Decay (ms) | 3.3 ± 0.4# | 3.7 ± 0.6 | 4.5 ± 1.3 | F(2,44) = 7.05, p < 0.01 | |
n (mice/cells) | 4/16 | 4/15 | 4/14 | ||
mIPSC | IEI (ms) | 3129.0 ± 3391.5* | 378.7 ± 433.3 | 120.5 ± 45.7 | F(2,44) = 10.3, p < 0.01 |
Amplitude (pA) | 40.1 ± 8.1 | 43.1 ± 7.3 | 35.8 ± 6.2 | F(2,44) = 3.67, p = 0.64 | |
Decay (ms) | 6.8 ± 1.5 | 6.3 ± 0.8 | 6.3 ± 0.9 | F(2,44) = 1.15, p = 0.33 | |
n (mice/cells) | 4/16 | 4/15 | 4/14 | ||
Intrinsic properties | Whole-cell capacitance (pF) | 33.6 ± 6.1 | 30.4 ± 6.7 | 30.6 ± 6.9 | F(2,44) = 1.15, p = 0.33 |
Input resistance (MΩ) | 148 ± 68 | 115 ± 38 | 100 ± 37 | F(2,44) = 3.61, p = 0.04 | |
Membrane time constant (τw, ms) | 1.9 ± 0.6 | 2.1 ± 0.6 | 2.5 ± 0.8 | F(2,44) = 2.55, p = 0.09 |
↵* Significant difference between AAV-DTR + DT and both AAV-DTR alone and DT alone (one-way ANOVA, Tukey’s multiple comparison test).
#Significant difference between AAV-DTR + DT and DT alone (one-way ANOVA, Tukey’s multiple comparison test).