Interneurons and motor neurons examined for osmotic upshifts
| Transgene | Affected neurons | Number of turns/min for the 5th minute | p value | |||
|---|---|---|---|---|---|---|
| Nontransgenic control | Transgenic worms | |||||
| 150 mOsm | 400 mOsm | 150 mOsm | 400 mOsm | |||
| Podr-2b(3a)::twk-18(gf) | AIB, AIZ | 9.38 ± 1.19 (16) | 25.69 ± 1.96 (16) | 5.63 ± 1.38 (16) | 22.60 ± 2.24 (15) | 0.848 |
| Pinx-1::twk-18(gf) | AIB | 7.44 ± 1.19 (16) | 29.31 ± 2.22 (16) | 1.63 ± 0.51 (16) | 26.56 ± 2.04 (16) | 0.355 |
| Pttx-3::twk-18(gf) | AIY | 7.81 ± 0.99 (16) | 25.06 ± 2.26 (16) | 7.56 ± 1.08 (16) | 23.40 ± 1.92 (15) | 0.669 |
Values are reported as the mean ± SEM (n animals tested). Transgenic animals that express a gain-of-function isoform of the potassium channel TWK-18 to inhibit the activity of specific interneurons and/or motor neurons are examined. The number of turns per minute is shown for the 5th minute for each genotype (transgenic vs nontransgenic siblings) and osmolarity treatment. The interaction of genotype and treatment is tested with two-way ANOVA on the number of turns per minute in the 5th minute. The number of animals tested under each condition is indicated in the parenthesis next to mean ± SEM.