Table 2.

Quantification of rabies-labeled presynaptic inputs to dentate granule cells

	Starter neuron	DG GCL	DG ML	DG Hilus	CA1 non-SP	CA3a SP	CA3a non-SP	CA3b SP	CA3b non-SP	CA3c SP	CA3c non-SP	PRh	LEnt	mEnt	MS-DB	Subicu-lum	SuM	Raphe
A. Individual case summary
Case 1711 (a total of 2421 neuron counts)
Number of neurons	128	23	10	261	13	125	24	80	24	33	7	40	1285	286	182	8	15	1
CSI		0.18	0.08	2.04	0.10	0.98	0.19	0.63	0.19	0.26	0.05	0.31	10.04	2.23	1.42	0.06	0.12	0.01
% of total labeling		0.95	0.41	10.78	0.54	5.16	0.99	3.30	0.99	1.36	0.29	1.65	53.08	11.81	7.52	0.33	0.62	0.04
Case 1713 (a total of 1810 neuron counts)
Number of neurons	119	18	7	168	18	48	8	34	24	12	9	73	1048	165	161	8	7	2
CSI		0.15	0.06	1.41	0.13	0.40	0.07	0.29	0.20	0.10	0.08	0.61	8.81	1.39	1.35	0.07	0.06	0.02
% of total labeling		0.99	0.39	9.28	1	2.65	0.44	1.88	1.33	0.66	0.50	4.03	57.90	9.12	8.90	0.44	0.39	0.11
Case 1868 (a total of 3192 neuron counts)
Number of neurons	228	35	16	421	29	147	29	96	35	53	17	77	1669	315	196	10	35	5
CSI		0.15	0.07	1.85	0.13	0.64	0.13	0.42	0.15	0.23	0.07	0.34	7.32	1.38	0.86	0.04	0.15	0.02
% of total labeling		1.10	0.50	13.19	0.91	4.61	0.91	3.01	1.10	1.66	0.53	2.41	52.29	9.87	6.14	0.31	1.10	0.16
Case 1867 (a total of 1441 neuron counts)
Number of neurons	88	15	11	137	28	63	29	39	24	32	6	42	693	198	106	4	7	0
CSI		0.17	0.13	1.56	0.30	0.72	0.33	0.44	0.27	0.36	0.07	0.48	7.88	2.25	1.20	0.05	0.08	0.00
% of total labeling		1.04	0.76	9.51	1.94	4.37	2.01	2.71	1.67	2.22	0.42	2.91	48.09	13.74	7.36	0.28	0.49	0.00
Case 1864 (a total of 509 neuron counts)
Number of neurons	32	5	7	45	8	17	5	10	4	7	2	9	269	66	52	1	2	0
CSI		0.16	0.22	1.41	0.25	0.53	0.16	0.31	0.13	0.22	0.06	0.28	8.41	2.06	1.63	0.03	0.06	0.00
% of total labeling		0.98	1.38	8.84	1.57	3.34	0.98	1.96	0.79	1.38	0.39	1.77	52.85	12.97	10.22	0.20	0.39	0.00

B. Summary data of all 5 cases
	DG GCL	DG ML	DG Hilus	CA1 non-SP	CA3a SP	CA3a non-SP	CA3b SP	CA3b non-SP	CA3c SP	CA3c non-SP	PRh	LEnt	mEnt	MS-DB	Subicu-lum	SuM	Raphe
CSI (Mean)	0.16	0.11	1.65	0.18	0.65	0.17	0.42	0.19	0.23	0.07	0.40	8.49	1.86	1.29	0.05	0.09	0.01
SE	0.01	0.03	0.13	0.04	0.10	0.04	0.06	0.03	0.04	0.00	0.06	0.46	0.20	0.13	0.01	0.02	0.00
% of total labeling	1.01	0.69	10.32	1.19	4.03	1.07	2.57	1.17	1.46	0.43	2.56	52.84	11.50	8.03	0.31	0.60	0.06
SE	0.03	0.18	0.79	0.25	0.45	0.26	0.28	0.15	0.25	0.04	0.43	1.56	0.88	0.70	0.04	0.13	0.03

As every one in three sections across the whole brain series were used for mapping rabies-labeled presynaptic neurons of targeted dentate granule cells, the actual total number of labeled neurons in each case would be estimated three times of the total neuron counts listed in the table. The labeled neurons are predominantly distributed in the ipsilateral hemisphere. The neuronal count for each structure in each case is pooled from both hemispheres if there are any labeled neurons in the contralateral hemisphere. The percentage of total labeled neurons is calculated as the percentage of the number of labeled presynaptic neurons in a specified structure versus the overall total presynaptic neuron count in each case. The input CSI is defined as the ratio of the number of labeled presynaptic neurons in a specified structure versus the number of starter neurons.
DG GCL, DG granule cell layer; DG ML, DG molecular layer; CA1 non-SP, CA1 nonpyramidal cell layers; CA3a-c SP, CA3a-c pyramidal cell layer; CA3a-c non-SP, CA3a-c nonpyramidal cell layer; LEnt, lateral EC; mEnt, medial EC; SuM, supramammillary nucleus; Raphe, raphe nuclei.