Statistical analyses
| Graph | Statistical test | Power | Factor effect | Interaction effect | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Antidepressant effects | Factor treatment | Factor experimental phase | ||||||||
| a. | Fig. 1A | FST | One-way ANOVA | n = 10 | F(2,27) = 16.20; | p < 0.001 | ||||
| b. | Fig. 1C | FUST controls | Two-way RM ANOVA | n = 8 | F(1,14) = 0.11; | p > 0.05 | F(2,28) = 3.09; | p > 0.05 | F(2,28) = 0.03; | p > 0.05 |
| c. | Fig. 1B | FUST | Two-way RM ANOVA | n = 9-10 | F(3,33) = 2.91; | p < 0.05 | F(2,66) = 39.97; | p < 0.001 | F(6,66) = 2.34; | p = 0.05 |
| Effects on cortical oscillations | Factor pretreatment | Factor time | ||||||||
| d. | Fig. 2C | γ power | Two-way RM ANOVA | n = 8-9 | F(1,15) = 20.07; | p < 0.001 | F(11,165) = 8.49; | p < 0.001 | F(11,165) = 7.05; | p < 0.001 |
| e. | Fig. 2D | α power | Two-way RM ANOVA | n = 8-9 | F(1,15) = 2.71; | p > 0.05 | F(11,165) = 2.09; | p < 0.05 | F(11,165) = 2.21; | p < 0.05 |
| f. | Fig. 2E | β power | Two-way RM ANOVA | n = 8-9 | F(1,15) = 2.83; | p > 0.05 | F(11,165) = 2.12; | p < 0.05 | F(11,165) = 1.29; | p > 0.05 |
| g. | Fig. 2F | δ power | Two-way RM ANOVA | n = 8-9 | F(1,15) = 0.0001; | p > 0.05 | F(11,165) = 0.64; | p > 0.05 | F(11,165) = 0.81; | p > 0.05 |
| h. | Fig. 2G | θ power | Two-way RM ANOVA | n = 8-9 | F(1,15) = 2.24; | p > 0.05 | F(11,165) = 2.09; | p < 0.05 | F(11,165) = 1.43; | p > 0.05 |
| NBQX effects, FST | Factor treatment | Factor pretreatment | ||||||||
| i. | Fig. 3 | 1 h postinjection | Two-way ANOVA | n = 9 | F(1,24) = 10.75; | p < 0.01 | F(1,24) = 3.92; | p = 0.06 | F(1,24) = 8.87; | p < 0.01 |
| j. | 24 h postinjection | Two-way ANOVA | n = 9 | F(1,24) = 8.82; | p < 0.01 | F(1,24) = 2.37; | p > 0.05 | F(1,24) = 6.95; | p < 0.05 | |
| Effects DMSO on behavior | Factor treatment | Factor time | ||||||||
| k. | Fig. 4A | Open-field test (timeline) | Two-way RM ANOVA | n = 9 | F(1,16) = 0.37; | p > 0.05 | F(23,368) = 53.80; | p < 0.001 | F(23,368) = 1.20; | p < 0.01 |
| l. | Open-field test (bar graph) | Unpaired t test (two-tailed) | n = 9 | t = 0.15; df = 16 | p > 0.05 | |||||
| m. | Fig. 4B | FST | Unpaired t test (two-tailed) | n = 10 | t = 0.45; df = 18 | p > 0.05 | ||||
| Factor treatment | Factor dB | |||||||||
| n. | Fig. 4C | PPI | Two-way RM ANOVA | n = 17 | F(1,32) = 0.001 | p > 0.05 | F(2,64) = 16.18; | p < 0.001 | F(2,64) = 0.80; | p > 0.05 |
| o. | Fig. 4D | Startle amplitude | Unpaired t test (two-tailed) | n = 17 | t = 1.0; df = 28 | p > 0.05 | ||||
| Factor treatment | Factor time | |||||||||
| p. | Fig. 4E | Rota-rod | Two-way RM ANOVA | n = 8 | F(1,15) = 0.30; | p > 0.05 | F(5,75) = 1.36; | p < 0.001 | F(5,75) = 1.15; | p < 0.01 |
| Side effects | Factor treatment | Factor time | ||||||||
| q. | Fig. 5A | Open-field test (timeline) | Two-way RM ANOVA | n = 8 | F(1,24) = 8.82; | p < 0.01 | F(1,24) = 2.37; | p > 0.05 | F(1,24) = 6.95; | p < 0.05 |
| r. | Open-field test (bar graph) | Unpaired t test (two-tailed) | n = 8 | F(1,24) = 8.82; | p < 0.01 | F(1,24) = 2.37; | p > 0.05 | F(1,24) = 6.95; | p < 0.05 | |
| s. | Fig. 5B | Rota-rod | Two-way RM ANOVA | n = 8-9 | F(3,30) = 12.28; | p < 0.001 | F(5,150) = 11.46; | p < 0.001 | F(15,150) = 10.78; | p < 0.001 |
| t. | Fig. 5C | CPP | Two-way RM ANOVA | n = 10 | F(2,27) = 3.32; | p = 0.05 | F(1,27) = 0.80; | p > 0.05 | F(2,27) = 7.33; | p < 0.01 |
| Factor treatment | Factor dB | |||||||||
| u. | Fig. 5D | PPI | Two-way RM ANOVA | n = 6-8 | F(3,24) = 7.42; | p < 0.01 | F(2,48) = 19.47; | p < 0.001 | F(6,48) = 0.62; | p > 0.05 |