Statistical table
| Figure | Data structure | Type of test | Power |
|---|---|---|---|
| 1D | Quantification of DNA by qPCR (n = 4–6, 2 biological replicates with each having 2–3 technical replicates) | One-way ANOVA followed by Student’s t test (two-tailed, type 2) | *p < 0.05, **p < 1 × 10−4 |
| 3A | Quantification of DNA by qPCR (n = 18, 6 biological replicates with each having 3 technical replicates) | One-way ANOVA followed by Student’s t test (two-tailed, type 2) | *p < 0.05, **p < 1 × 10−4, ***p < 1 × 10−5 |
| 3B | Measurement of luciferase reporter activity (n = 9, 3 biological replicates with each having 3 technical replicates) | Gaussian distribution was confirmed by D’Agostino–Pearson normality test (α = 0.05); one-way ANOVA followed by post hoc Tukey’s test | ****p < 0.0001 |
| 4B | Quantification of mRNA by RT-qPCR (n = 9, 3 biological replicates, with each having 3 technical replicates) | Gaussian distribution was confirmed by D’Agostino–Pearson normality test (α = 0.05); one-way ANOVA followed by post hoc Tukey’s test | *p = 0.023 |
| 4D | Quantification of protein levels by immunoblotting (n = 3 biological replicates) | Unpaired t test (two-tailed, type 2) | p = 0.057 |
| 4G,H | Quantification of protein levels by immunofluorescence (n = 30, from 2 biological replicates) | Nonparametric Kruskal–Wallis test followed by Dunn’s multiple-correction analysis | *p < 0.02,**p = 0.0012, ****p < 0.0001 |
| 6B–F | Quantification of calcium levels by measuring Fluo-3 signal (n = 20–50 per condition) | Nonparametric Kruskal–Wallis test followed by Dunn’s multiple-correction analysis | **p = 0.0073, ***p = 0.0008 |
| 7B–F | Quantification of calcium levels by measuring Fluo-3 signal (n = 20–40 per condition) | Unpaired t test (two-tailed, type 2) | *p < 0.05 |