Analyte-dependent parameters for the transitions used in scheduled multiple reaction monitoring data acquisition
| Analyte | Q1 (Da) 12C analyte | Q3 (Da) 12C analyte | RT (min) | DP (volts) | CE (volts) | CXP (volts) |
|---|---|---|---|---|---|---|
| Glucose 6-phosphate | 258.89 | 96.7 | 8.1 | –20 | –30 | –15 |
| Fructose 6-bisphosphate | 259.02 | 96.8 | 9.6 | –20 | –30 | –15 |
| Fructose 1,6-bisphosphate | 339.08 | 96.9 | 21.9 | –20 | –30 | –15 |
| Dihydroxyacetone phosphate | 168.84 | 97 | 11.9 | –50 | –14 | –5 |
| 2- and 3-Phosphoglycerate | 184.91 | 97 | 21.5 | –50 | –20 | –5 |
| Phosphoenolpyruvate | 166.83 | 79 | 22.3 | –40 | –18 | –5 |
| Pyruvate | 87.02 | 43 | 11.9 | –45 | –12 | –1 |
| Citrate | 190.96 | 110.9 | 22.6 | –50 | –18 | –7 |
| Aconitate | 172.94 | 84.9 | 22.6 | –30 | –18 | –5 |
| 2-Oxoglutarate | 144.95 | 100.8 | 20.5 | –40 | –12 | –5 |
| Succinate | 117 | 73 | 18.5 | –45 | –16 | –3 |
| Fumarate | 115.01 | 70.9 | 21.1 | –45 | –12 | –1 |
| Malate | 133 | 70.8 | 19.7 | –40 | –22 | –3 |