Counts of Tph2-immunopositive and EphA5-expressing neurons and colocalized neurons in different raphe nuclei
| Neuron | B1 | B2 | B3 | B5 | B6 | B7 | B8 | B9 |
|---|---|---|---|---|---|---|---|---|
| Tph2+ | 8.2 ± 0.9 | 2.8 ± 0.1 | 7.4 ± 0.9 | 5.1 ± 0.1 | 30.7 ± 1.9 | 11.2 ± 0.9 | 5 ± 0.4 | 7.3 ± 0.5 |
| EphA5+ | 19.3 ± 3.3 | 3.3 ± 1.7 | 2.9 ± 1.5 | 8.9 ± 0.2 | 41.2 ± 2.9 | 23.3 ± 2.2 | 18.9 ± 1.6 | 16.9 ± 0.8 |
| Tph2+/EphA5+ | 1.3 ± 0.2 | 0 | 0.1 ± 0.1 | 0.7 ± 0.1 | 11.5 ± 0.6 | 5.7 ± 0.4 | 1.1 ± 0.1 | 1.6 ± 0.2 |
| Tph2 colocalized, % | 16.4 ± 1 | 0 | 1.8 ± 1.9 | 14.3 ± 0.7 | 37.6 ± 1.6 | 50.5 ± 1.2 | 22.3 ± 0.2 | 22.3 ± 2.8 |
Data are mean cell numbers ± SEM/area obtained from counts done in three cases in ROI covering the different raphe nuclei (B1–B9) . All cells (validated by DAPI staining) immunolabeled for Tph2, for EphA5 and double-labeled for Tph2 and EhpA5 were counted in three different ROIs (dimension 0.225 mm2) for each structure and for each case and checked for colocalization. Percentage of the colabeled Tph2 neurons is indicated.