List of antibodies.
| Antibody | Manufacturer (species, cat. no.) | Concentrations (primary; secondary) | Blocking solution |
|---|---|---|---|
| AKT | Cell Signaling Technology; 4685 RRID:AB_2225340 | 1:5000; 1:5000 | 5% Milk/TBST |
| pERK T202/Y204 | Cell Signaling Technology; 4370 RRID:AB_2315112 | 1:10,000; 1:5000 | 5% BSA/TBST |
| ERK | Santa Cruz Biotechnology; sc-94 RRID:AB_2140110 | 1:20,000; 1:10,000 | 5% Milk/TBST |
| pFMRP S499 | PhosphoSolutions; p1125-499 RRID:AB_2492094 | 1:1000; 1:2000 | Block milk 5%, probe in BSA (pFMRP must be probed for before FMRP) |
| FMRP | Abcam; ab17722 RRID:AB_2278530 | 1:5000; 1:5000 | 5% Milk/TBST |
| pS6 S240/244 | Cell Signaling Technology; 5364 RRID:AB_10694233 | 1:10,000; 20,000 | 5% BSA/TBST (total S6 must be probed for first, as pS6 is not efficiently stripped) |
| Total S6 | Cell Signaling Technology; 2217 RRID:AB_331355 | 1:5000; 1:10,000 | 5% Milk/TBST |
| Phosphoserine antibody | Millipore; AB1603 RRID:AB_390205 | 1:2000; 1:2000 | See Protocols.io |
| Phosphothreonine HRP conjugate antibody | Cell Signaling Technology; 6949S RRID:AB_10828224 | 1:1000; no secondary | See Protocols.io |
| Phosphotyrosine antibody | Cell Signaling Technology (Rb) #8954 | 1:1000; 1:2000 | See Protocols.io |
| Goat anti-mouse IgG–HRP secondary | Santa Cruz Biotechnology; sc-2005 RRID:AB_631736 | 1:10,000 | 5% Milk/TBST |
| Anti-rabbit IgG secondary | Cell Signaling Technology; 7074 RRID:AB_2099233 | 1:5000 | 5% Milk/TBST |