TH-siRNA, Ctrl-siRNA, TH-shRNA, and Ctrl-shRNA sequence, primers
| Name | Sequence (accession number) |
|---|---|
| TH-siRNA | Sense strand- 5'CUGUGAAGUUUGACCCGUAtt-3' (NM_012740) |
| Antisense strand-5'UACGGGUCAAACUUCACAGgg-3' (NM_012740) | |
| Ctrl-siRNA | Non-targeting control (Ambion, 4390843) |
| TH-shRNA | 5'CCGGCAGGAACTATGCCTCTCGTATCTCGAGATA CGAGAGGCATAGTTCCTGTTTTTG 3' (TRCN0000115525 NM_009377.1-1340s1c1) |
| Ctrl-shRNA | Turbo GFP shRNA control particles (SHC004V) |
| TH-Forward primer | 5'GTATATCCGCCATGCCTCCT3' NM_012740.3 |
| TH-Revers primer | 5'AATGTCCTGGGAGAACTGGG3' NM_012740.3 |
| TBP-Forward primer | 5'ACCTAAAGACCATTGCACTTCG3' NM_001004198 |
| TBP-Reverse primer | 5'GCTCTCTTATTCTCATGATGACTGC3' NM_001004198 |
| GAPDH-Forward primer | 5'AGGTCGGTGTGAACGGATTTG3' NM_017008 |
| GAPDH-Reverse primer | 5'TGTAGACCATGTAGTTGAGGTCA3' NM_017008 |