Table 2.

qRT-PCR validation of select microarray candidates shows interplatform reproducibility.

Gene symbolGene nameFCp-valueFC
High in ipsilateral RGCs
    Gja1Gap junction protein, alpha 1 (connexin 43)17.600.001>500
    Klf4Kruppel–like factor 47.380.002455
    Lhx2LIM homeobox protein 24.720.0092.37
    Otx2Orthodenticle homolog 25.020.0210.651
    Slc6a4Serotonin transporter (SERT)4.490.00449.8
    Sox2SRY–box containing gene 27.470.0293.81
    SparcSecreted acidic cysteine rich glycoprotein12.470.0006.02
    Zfp36Zinc finger protein 368.280.00231.2
    Zic1Zinc finger protein of the cerebellum 15.670.0026.49
    Zic2Zinc finger protein of the cerebellum 25.780.01664.7
High in contralateral RGCs
    Sema3eSemaphorin 3E–2.200.003–2.52
    Tbx20T–box 20–2.150.001–42.5
  • Of these 11 genes, all but Otx2 (italicized) were enriched in the RGC subpopulation as seen by gene profiling, demonstrating interplatform reproducibility of the gene expression detection by microarray. Genes previously known to be differentially expressed in ipsilateral and contralateral RGCs are bolded (Zic2, SERT).