Quantification of Ctbp2-labeled ribbons.
| Type | Number/25 μm2 | |||||
|---|---|---|---|---|---|---|
| Ribbons in OPL | p-value | Ribbons in NK3R-labeled outer IPL | p-value | Ribbons in Syt2-labeled inner IPL | p-value | |
| WT | 8.58 ± 0.30 | 1 | 16.33 ± 0.45 | 1 | 13.33 ± 0.42 | 1 |
| Cabp1–/– | 8.12 ± 0.27 | 0.26 | 16.04 ± 0.49 | 0.66 | 14.16 ± 0.53 | 0.22 |
| Cabp2–/– | 8.25 ± 0.21 | 0.36 | 16.75 ± 0.47 | 0.53 | 14.54 ± 0.53 | 0.08 |
Retinal whole mount, as shown in Figures 4 and 6, from four mice for each phenotype were analyzed by confocal microscopy. Ctbp2-labeled ribbons were counted in images of six areas of 25 μm2 for each mouse in the OPL. Ctbp2-labeled puncta were also quantified in the NK3R-labeled sublamina containing type 1 and 2 OFF bipolar cell axon terminals or Syt2-labeled sublaminae of the inner IPL containing the type 6 ON bipolar cells. Numbers represent mean ± SE (n = 24). Statistical comparisons with WT mice were performed using the Student’s t-test.