Statistical analyses performed in all experiments
| Figure | Data structure | Type of test/comparisons | p Value |
|---|---|---|---|
| 1A | See below | ||
| 1B | Normal distribution | Two-way ANOVA (stress treatment × virus type) | Main effect of virus type: p = 0.0044 |
| Main effect of stress treatment: p < 0.0001 | |||
| Sidak’s multiple-comparisons test | Interaction: p < 0.0001 | ||
| Home cage–inescapable Stress | 95% CI: | ||
| eYFP | 30.24–58.97 | ||
| NpHR | −7.976 to 20.10 | ||
| 2B | Normal distribution | Two-way repeated-measures ANOVA | Main effect virus type: p = 0.0007 |
| Sidak’s multiple comparisons test | Main effect time: p < 0.0001 | ||
| eYFP – NpHR | Interaction: p = 0.0002 | ||
| BL1 | 95% CI: | ||
| BL2 | −43.11 to 65.40 | ||
| BL3 | −59.67 to 48.84 | ||
| BL4 | −47.73 to 60.78 | ||
| S1 | −66.85 to 41.66 | ||
| S2 | 33.82–142.3 | ||
| S3 | −9.440 to 99.07 | ||
| S4 | 7.267–115.8 | ||
| S5 | 1.613–110.1 | ||
| PS1 | 0.8602–109.4 | ||
| PS2 | −5.678 to 102.8 | ||
| PS3 | 13.43–121.9 | ||
| PS4 | 2.628–111.1 | ||
| Independent samples t test (AUC) | 0.5271–109.0 | ||
| T, df | p = 0.0023 | ||
| t = 3.859 df = 12.00 | |||
| 2C | Normal distribution | Independent-samples t test for baseline JSI 5-HT values | Maine effect virus type: p = 0.0029 |
| Two-way repeated-measures ANOVA | Main effect time: p < 0.0001 | ||
| Sidak’s multiple-comparisons test | Interaction: p < 0.0001 | ||
| eYFP – NpHR | 95% CI: | ||
| BL1 | −31.60 to 60.99 | ||
| BL2 | −32.35 to 60.23 | ||
| BL3 | −67.83 to 24.76 | ||
| BL4 | −41.90 to 50.69 | ||
| JSI | 78.49–171.1 | ||
| PI1 | −9.353 to 83.23 | ||
| PI2 | −34.55 to 58.03 | ||
| PI3 | −25.61 to 66.98 | ||
| Independent-samples t test (AUC) | p = 0.0102 | ||
| T, df | t = 3.580 df = 6.479 | ||
| 2D | Normal distribution | Independent-samples t test | p < 0.0001 |
| T, df | t = 6.344 df = 9.716 | ||
| 2D | Normal distribution | Linear regression | p = 0.0125 |
| R2 = 0.3921 | |||
| R = −0.6257 | |||
| T, df | t = 6.344 df = 9.716 | ||
| 3 | Normal distribution | Ordinary one-way ANOVA | p = 0.0065 |
| Tukey’s post hoc test | 95% CI | ||
| MHb Fos 0 h | 0.03556 to 2.589 | ||
| HC vs ES | 0.4731 to 3.027 | ||
| HC vs IS | -0.8394 to 1.714 | ||
| ES vs IS | p < 0.0001 | ||
| LHbM Fos 0 h | −37.59 to −19.54 | ||
| HC vs ES | −34.15 to −16.10 | ||
| HC vs IS | −5.587 to 12.46 | ||
| ES vs IS | p = 0.1416 | ||
| LHbL Fos 0 h | p = 0.3300 | ||
| MHb Fos 2 h | p = 0.0013 | ||
| LHbM Fos 2 h | 95% CI: | ||
| HC vs ES | −38.21 to −8.789 | ||
| HC vs IS | −34.40 to −4.977 | ||
| ES vs IS | −10.90 to 18.52 | ||
| LHbL Fos 2 h | p = 0.1888 | ||
| MHb Fos 4 h | p = 0.8780 | ||
| LHbM Fos 4 h | p = 0.8652 | ||
| LHbL Fos 4 h | p = 0.0348 | ||
| HC vs ES | 95% CI: | ||
| HC vs IS | −3.706 to 0.4562 | ||
| ES vs IS | −4.331 to −0.1688 | ||
| −2.706 to 1.456 | |||
| 4 | Normal distribution | Ordinary one-way ANOVA | 95% CI: |
| Tukey’s post hoc test | p = 0.6651 | ||
| Total FG MHb | p = 0.7163 | ||
| Total FG LHbM | p = 0.5220 | ||
| Total FG LHbL | p = 0.9074 | ||
| Total Fos-IR MHb | p < 0.0001 | ||
| Total Fos-IR LHbM | 95% CI: | ||
| HC vs ES | −16.94 to −6.726 | ||
| HC vs IS | −18.73 to −8.517 | ||
| ES vs IS | −6.899 to 3.316 | ||
| Total Fos-IR LHbL | p = 0.0109 | ||
| HC vs ES | 95% CI: | ||
| HC vs IS | −8.395 to −1.022 | ||
| ES vs IS | −7.020 to 0.3534 | ||
| Total % double-label MHb | −2.312 to 5.062 | ||
| Total % double-label LHbM | p = 0.4152 | ||
| HC vs ES | p < 0.0001 | ||
| HC vs IS | 95% CI: | ||
| ES vs IS | −21.49 to −7.661 | ||
| Total % double-label LHbL | −18.44 to −4.614 | ||
| −3.866 to 9.960 | |||
| p = 0.0764 |