Values of Auto Bias for iTRAQ analysis in ProteinPilot. Observed iTRAQ ratios of all peptides were normalized by using Auto Bias. Gel, a part of gel part in Figure 1C.
| Gel | Auto bias (median) | ||
|---|---|---|---|
| 115/114 | 116/114 | 117/114 | |
| 1 | 1.3163 | 1.0467 | 1.0604 |
| 2 | 1.2254 | 1.0641 | 0.8925 |
| 3 | 0.9273 | 0.9753 | 1.0206 |
| 4 | 0.7433 | 1.0111 | 0.9129 |
| 5 | 0.998 | 0.9869 | 0.9197 |
| 6 | 0.9157 | 0.9237 | 0.9097 |