PCR primer description and sequences
| Gene | Primer sequence: 5' → 3' | Function |
|---|---|---|
| β-Actin | F: TTCCTTCCTGGGTATGGAAT R: GAGGAGCAATGATCTTGATC | Cytoskeletal protein (housekeeping gene) |
| IL-1β | F: CCTTGTGCAAGTGTCTGAAG R: GGGCTTGGAAGCAATCCTTA | Proinflammatory cytokine |
| IL-6 | F: AGAAAAGAGTTGTGCAATGGCA R: GGCAAATTTCCTGGTTATATCC | Proinflammatory cytokine |
| IκBα | F: CACCAACTACAACGGCCACA R: GCTCCTGAGCGTTGACATCA | Marker for transcription factor NF-κB activity |
| CD11b | F: CTGGGAGATGTGAATGGAG R: ACTGATGCTGGCTACTGATG | Macrophage/microglial antigen marker |
| HMGB1 | F: GAGGTGGAAGACCATGTCTG R: AAGAAGAAGGCCGAAGGAGG | Endogenous danger signal |
| NLRP3 | F: AGAAGCTGGGGTTGGTGAATT R: GTTGTCTAACTCCAGCATCTG | Rate limiting protein in NLRP3 inflammasome formation |
| CX3CR1 | F: TCAGGACCTCACCATGCCTA R: CGAACGTGAAGACAAGGGAG | Microglia-selective chemokine receptor |
| TLR4 | F: TCCCTGCATAGAGGTACTTC R: CACACCTGGATAAATCCAGC | Pattern recognition receptor |
CD, cluster of differentiation; F, forward; R, reverse.