Table 4.

Tabulation of all proteins identified in common between two biological replicates of eight-plex iTRAQ-labeled β2*-nAChRs immunoisolated with mAb295 from mouse cortex, indicating which proteins have been identified in human temporal cortex or whole mouse brain (Iso, isoform of protein identified; GN, gene name)

Accession_MOUSEDescriptionHuman CtxMouse brain
1433T14-3-3 protein theta GN = YwhaqIsoIso
ACHA4Neuronal acetylcholine receptor subunit α4 GN = Chrna4YesYes
ACHA5Neuronal acetylcholine receptor subunit α5 GN = Chrna5IsoIso
ACHB2Neuronal acetylcholine receptor subunit β2 GN = Chrnb2YesYes
ACPMAcyl carrier protein, mito GN = Ndufab1NoYes
ACTG; ACTBActin, cytoplasmic 2 GN = Actg1; actin, cytoplasmic 1 GN = ActbIsoYes
ADT1ADP/ATP translocase 1 GN = Slc25a4 SV = 4YesYes
ADT2ADP/ATP translocase 2 GN = Slc25a5YesYes
AINXα-Internexin GN = InaNoYes
AT1A3Sodium/potassium-transporting ATPase subunit α3 GN = Atp1a3YesYes
AT1B1Sodium/potassium-transporting ATPase subunit β1 GN = Atp1b1YesYes
AT5F1ATP synthase subunit b, mito GN = Atp5f1IsoYes
ATP5HATP synthase subunit d, mito GN = Atp5hIsoYes
ATP5IATP synthase subunit e, mito GN = Atp5iIsoYes
ATPAATP synthase subunit α, mito GN = Atp5a1YesYes
ATPBATP synthase subunit β, mito GN = Atp5bYesYes
ATPDATP synthase subunit delta, mito GN = Atp5dIsoYes
C1QAComplement C1q subcomponent subunit A GN = C1qaNoNo
CALMCalmodulin GN = Calm1YesYes
CLH1Clathrin heavy chain 1 GN = CltcYesNo
CMC1Calcium-binding mito carrier protein Aralar1 GN = Slc25a12NoYes
CN372',3'-cyclic-nucleotide 3'-phosphodiesterase GN = CnpNoYes
EAA2Excitatory amino acid transporter 2 GN = Slc1a2YesYes
G3PGlyceraldehyde-3-phosphate dehydrogenase GN = GapdhYesYes
GBB1Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit β1 GN = Gnb1YesYes
GFAPGlial fibrillary acidic protein GN = Gfap SV = 4YesYes
GLNAGlutamine synthetase GN = Glul SV = 6NoYes
GNAOGuanine nucleotide-binding protein G(o) subunit α GN = Gnao1YesYes
KCC2ACalcium/calmodulin-dependent protein kinase type II subunit α GN = CaMKIIaYesYes
KCC2BCalcium/calmodulin-dependent protein kinase type II subunit β GN = CaMKIIbYesYes
M2OMMito 2-oxoglutarate/malate carrier protein GN = Slc25a11NoYes
MBPMyelin basic protein GN = MbpYesYes
MPCPPhosphate carrier protein, mito GN = Slc25a3YesYes
MYH10Myosin-10 GN = Myh10YesYes
MYL6Myosin light polypeptide 6 GN = Myl6YesYes
NDUA2NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 2 GN = Ndufa2IsoYes
NDUA4NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 4 GN = Ndufa4YesIso
NDUA5NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 5 GN = Ndufa5IsoYes
NDUA6NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 6 GN = Ndufa6IsoYes
NDUA7NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 7 GN = Ndufa7IsoYes
NDUA9NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 9, mito GN = Ndufa9IsoYes
NDUAANADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 10, mito GN = Ndufa10IsoYes
NDUACNADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 12 GN = Ndufa12IsoYes
NDUADNADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 13 GN = Ndufa13IsoYes
NDUB3NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 3 GN = Ndufb3IsoYes
NDUB4NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 4 GN = Ndufb4IsoYes
NDUB5NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 5, mito GN = Ndufb5IsoYes
NDUB6NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 6 GN = Ndufb6IsoYes
NDUB7NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 7 GN = Ndufb7IsoYes
NDUB8NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 8, mito GN = Ndufb8IsoYes
NDUB9NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 9 GN = Ndufb9IsoYes
NDUC2NADH dehydrogenase [ubiquinone] 1 subunit C2 GN = Ndufc2IsoYes
NDUS1NADH-ubiquinone oxidoreductase 75 kDa subunit, mito GN = Ndufs1IsoYes
NDUS2NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mito GN = Ndufs2IsoYes
NDUS3NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mito GN = Ndufs3YesYes
NDUS4NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mito GN = Ndufs4IsoYes
NDUS5NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 GN = Ndufs5IsoYes
NDUS6NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mito GN = Ndufs6IsoYes
NDUS7NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mito GN = Ndufs7IsoYes
NDUS8NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mito GN = Ndufs8IsoYes
NDUV1NADH dehydrogenase [ubiquinone] flavoprotein 1, mito GN = Ndufv1IsoYes
NDUV2NADH dehydrogenase [ubiquinone] flavoprotein 2, mito GN = Ndufv2YesYes
NESTNestin GN = NesNoNo
NFHNeurofilament heavy polypeptide GN = NefhIsoIso
NFLNeurofilament light polypeptide GN = Nefl SV = 5YesYes
NFMNeurofilament medium polypeptide GN = Nefm SV = 4YesYes
OCAD2OCIA domain-containing protein 2 GN = Ociad2NoYes
ODO2Dihydrolipoyl-lysine residue succinyltransferas 2-oxoglutarate dehydrogenase complex, mito GN = DlstYesYes
ODPAPyruvate dehydrogenase E1 component subunit α, somatic form, mito GN = Pdha1NoYes
ODPBPyruvate dehydrogenase E1 component subunit β, mito GN = PdhbNoYes
PHBProhibitin GN = PhbNoYes
PHB2Prohibitin-2 GN = Phb2NoYes
RAB3ARas-related protein Rab-3A GN = Rab3aIsoYes
SFXN3Sideroflexin-3 GN = Sfxn3NoYes
SNP25Synaptosomal-associated protein 25 GN = Snap25YesYes
SPTB2Spectrin β chain, nonerythrocytic 1 GN = Sptbn1YesYes
SPTN1Spectrin α chain, nonerythrocytic 1 GN = Sptan1 SV = 4YesYes
STX1BSyntaxin-1B GN = Stx1bYesYes
SYPHSynaptophysin GN = SypNoNo
SYT1Synaptotagmin-1 GN = Syt1YesYes
TBA4ATubulin α4A chain GN = Tuba4aYesYes
TBB2ATubulin β2A chain GN = Tubb2aYesYes
TBB3Tubulin β3 chain GN = Tubb3YesYes
TBB4ATubulin β4A chain GN = Tubb4aYesYes
TBB4BTubulin β4B chain GN = Tubb4bYesYes
TBB5Tubulin β5 chain GN = Tubb5YesYes
THY1Thy-1 membrane glycoprotein GN = Thy1YesNo
TPM3Tropomyosin α3 chain GN = Tpm3IsoNo
VA0D1V-type proton ATPase subunit d 1 GN = Atp6v0d1YesIso
VATAV-type proton ATPase catalytic subunit A GN = Atp6v1aYesYes
VDAC1Voltage-dependent anion-selective channel protein 1 GN = Vdac1NoYes
VDAC2Voltage-dependent anion-selective channel protein 2 GN = Vdac2NoYes
VIMEVimentin GN = VimNoYes
VPP1V-type proton ATPase 116 kDa subunit a isoform 1 GN = Atp6v0a1YesIso