Table 1.

Tabulation of all proteins identified from mAb295-immunoisolated β2*-nAChRs from human temporal cortex, indicating which proteins have been identified in mouse cortex or whole mouse brain (Iso, isoform of protein identified; GN, gene name)

Accession_HUMANDescriptionMouse CtxMouse Brain
1433E14-3-3 protein ε GN = YWHAEIsoIso
1433G;1433F14-3-3 protein γ GN = YWHAGIsoIso
1433S14-3-3 protein σ GN = SFNIsoIso
1433Z14-3-3 protein ζ/δ GN = YWHAZIsoYes
ACHA4;ACHA2;ACHA6Neuronal acetylcholine receptor subunit α4 GN = CHRNA4YesYes
ACHB2Neuronal acetylcholine receptor subunit β2 GN = CHRNB2YesYes
ACTB;ACTBM;POTEEActin, cytoplasmic 1 GN = ACTBYesIso
ACTC;ACTAActin, α cardiac muscle 1 GN = ACTC1IsoYes
ACTN1;ACTN3α-Actinin-1 GN = ACTN1NoYes
ACTN2α-Actinin-2 GN = ACTN2NoYes
ACTN4α-Actinin-4 GN = ACTN4NoIso
ADDAα-Adducin GN = ADD1NoNo
ADT1;ADT2;ADT4ADP/ATP translocase 1 GN = SLC25A4YesYes
ALBUSerum albumin GN = ALBNoNo
ANK2Ankyrin-2 GN = ANK2NoYes
AP1B1AP-1 complex subunit β1 GN = AP1B1NoNo
ARP2Actin-related protein 2 GN = ACTR2NoIso
ARP3Actin-related protein 3 GN = ACTR3NoYes
ARPC3Actin-related protein 2/3 complex subunit 3 GN = ARPC3NoIso
ARPC4Actin-related protein 2/3 complex subunit 4 GN = ARPC4NoIso
AT1A1;AT12ASodium/potassium-transporting ATPase subunit α1 GN = ATP1A1IsoIso
AT1A2Sodium/potassium-transporting ATPase subunit α2 GN = ATP1A2IsoYes
AT1A3;AT1A4;ATP4ASodium/potassium-transporting ATPase subunit α3 GN = ATP1A3YesYes
AT1B1Sodium/potassium-transporting ATPase subunit β1 GN = ATP1B1YesYes
AT2B2;AT2B1Plasma membrane calcium-transporting ATPase 2 GN = ATP2B2NoNo
ATPAATP synthase subunit α, mito GN = ATP5A1YesYes
ATPBATP synthase subunit β, mito GN = ATP5BYesYes
BASP1Brain acid-soluble protein 1 GN = BASP1NoNo
CALMCalmodulin GN = CALM1YesYes
CANB1Calcineurin subunit B type 1 GN = PPP3R1NoNo
CLH1Clathrin heavy chain 1 GN = CLTC SV = 5YesNo
CNTN1Contactin-1 GN = CNTN1NoYes
COX2Cytochrome c oxidase subunit 2 GN = MT-CO2NoYes
COX41Cytochrome c oxidase subunit 4 isoform 1, mito GN = COX4I1NoYes
COX6CCytochrome c oxidase subunit 6C GN = COX6CNoYes
CX6B1Cytochrome c oxidase subunit 6B1 GN = COX6B1NoYes
CX7A2Cytochrome c oxidase subunit 7A2, mito GN = COX7A2NoYes
DREBDrebrin GN = DBN1NoYes
DYN1Dynamin-1 GN = DNM1NoNo
E41L3Band 4.1-like protein 3 GN = EPB41L3NoNo
EAA1Excitatory amino acid transporter 1 GN = SLC1A3IsoIso
EAA2Excitatory amino acid transporter 2 GN = SLC1A2YesYes
EF1A1;EF1A2Elongation factor 1-α1 GN = EEF1A1NoNo
FCGBPIgGFc-binding protein GN = FCGBPNoNo
FRIHFerritin heavy chain GN = FTH1NoNo
FRILFerritin light chain GN = FTLNoNo
G3PGlyceraldehyde-3-phosphate dehydrogenase GN = GAPDHYesYes
GBB1;GBB2;GBB3;GBB4Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit β1 GN = GNB1YesYes
GELSGelsolin GN = GSNNoYes
GFAPGlial fibrillary acidic protein GN = GFAPYesYes
GNAO;GNA12Guanine nucleotide-binding protein G(o) subunit α GN = GNAO1YesYes
GPM6ANeuronal membrane glycoprotein M6-a GN = GPM6ANoNo
H13Histone H1.3 GN = HIST1H1DNoIso
H2A1BHistone H2A type 1-B/E GN = HIST1H2ABNoYes
H2B1M;H2B1AHistone H2B type 1-M GN = HIST1H2BMNoYes
H4Histone H4 GN = HIST1H4ANoYes
HBAHemoglobin subunit α GN = HBA1NoIso
HBB;HBDHemoglobin subunit β GN = HBBNoYes
HORNHornerin GN = HRNRNoNo
HS12AHeat shock 70 kDa protein 12A GN = HSPA12ANoIso
HS71LHeat shock 70 kDa protein 1-like GN = HSPA1LNoIso
HSPB1Heat shock protein β1 GN = HSPB1NoIso
IGHG4;IGHG2Ig γ-4 chain C region GN = IGHG4NoIso
IGHMIg μ chain C region GN = IGHMNoYes
IGKCIg κ chain C region GN = IGKCNoIso
KCC2A;KCC2DCalcium/calmodulin-dependent protein kinase type II subunit α GN = CAMK2AYesYes
KCC2B;KCC2GCalcium/calmodulin-dependent protein kinase type II subunit β GN = CAMKIIBYesYes
MBPMyelin basic protein GN = MBPYesYes
ML12A;MYL9Myosin regulatory light chain 12A GN = MYL12ANoIso
MOGMyelin-oligodendrocyte glycoprotein GN = MOGNoNo
MPCPPhosphate carrier protein, mito GN = SLC25A3YesYes
MYH10Myosin-10 GN = MYH10YesYes
MYH9;MYH11;MYH14Myosin-9 GN = MYH9IsoYes
MYL6;MYL6BMyosin light polypeptide 6 GN = MYL6YesYes
MYO5A;MYO5BUnconventional myosin-Va GN = MYO5ANoYes
MYPRMyelin proteolipid protein GN = PLP1NoYes
NDUA4Cytochrome c oxidase subunit NDUFA4 GN = NDUFA4YesIso
NDUS3NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mito GN = NDUFS3YesYes
NDUV2NADH dehydrogenase [ubiquinone] flavoprotein 2, mito GN = NDUFV2YesYes
NFLNeurofilament light polypeptide GN = NEFLYesYes
NFMNeurofilament medium polypeptide GN = NEFMYesYes
NSFVesicle-fusing ATPase GN = NSFNoYes
ODB2Lipoamide acyltransferase of branched-chain α-keto acid dehydrogenase mito GN = DBTNoYes
ODO2Dihydrolipoyllysine-residue succinyltransferase 2-oxoglutarate dehydrogenase mito GN = DLSTYesYes
QCR2Cytochrome b-c1 complex subunit 2, mito GN = UQCRC2NoYes
RAB1ARas-related protein Rab-1A GN = RAB1AIsoYes
RL40Ubiquitin-60S ribosomal protein L40 GN = UBA52NoNo
RO52E3 ubiquitin-protein ligase TRIM21 GN = TRIM21NoNo
S10A7;S1A7AProtein S100-A7 GN = S100A7NoNo
S10A8Protein S100-A8 GN = S100A8NoNo
S10A9Protein S100-A9 GN = S100A9NoNo
SIR2NAD-dependent protein deacetylase sirtuin-2 GN = SIRT2NoNo
SNP25Synaptosomal-associated protein 25 GN = SNAP25YesYes
SPTB2;SPTB1Spectrin β chain, nonerythrocytic 1 GN = SPTBN1YesYes
SPTN1Spectrin α chain, nonerythrocytic 1 GN = SPTAN1YesYes
SPTN2Spectrin β chain, nonerythrocytic 2 GN = SPTBN2IsoIso
SSBPSingle-stranded DNA-binding protein, mito GN = SSBP1NoNo
STX1ASyntaxin-1A GN = STX1AIsoIso
STX1B;STX2Syntaxin-1B GN = STX1BYesYes
STXB1Syntaxin-binding protein 1 GN = STXBP1NoYes
SV2ASynaptic vesicle glycoprotein 2A GN = SV2ANoNo
SYT1Synaptotagmin-1 GN = SYT1YesYes
TBA1A,C;TBA3C,E;TBA8Tubulin α1A chain GN = TUBA1AIsoYes
TBA1BTubulin α1B chain GN = TUBA1BIsoIso
TBA4A;TBA4B;TBAL3Tubulin α4A chain GN = TUBA4AYesYes
TBB2A;TBB1;TBB2BTubulin β2A chain GN = TUBB2AYesYes
TBB3Tubulin β3 chain GN = TUBB3YesYes
TBB4A;TBB6,8;TBB8LTubulin β4A chain GN = TUBB4AYesYes
TBB4BTubulin β4B chain GN = TUBB4BYesYes
TBB5Tubulin β chain GN = TUBBYesYes
THY1Thy-1 membrane glycoprotein GN = THY1YesNo
TMOD2Tropomodulin-2 GN = TMOD2NoYes
TPM4;TPM1Tropomyosin α4 chain GN = TPM4IsoNo
VA0D1;VA0D2V-type proton ATPase subunit d 1 GN = ATP6V0D1YesIso
VAMP3Vesicle-associated membrane protein 3 GN = VAMP3NoIso
VATAV-type proton ATPase catalytic subunit A GN = ATP6V1AYesYes
VISL1Visinin-like protein 1 GN = VSNL1NoNo
VPP1V-type proton ATPase 116 kDa subunit a isoform 1 GN = ATP6V0A1 PE = 2YesIso