Control | βERKD | ||||||
Total ERβ | Double-labeled | Total ERβ | Double-labeled | ||||
Bregma | Cell number | Cell number | % in total | Cell number | Cell number | % in total | |
MPOA | +0.02 | 20.5 ± 4.7 | 15.3 ± 3.6 | 76.2 ± 6.3 | 8.0 ± 2.0* | 0 | 0 |
-0.10 | 40.8 ± 8.2 | 32.0 ± 6.3 | 79.5 ± 3.8 | 5.0 ± 1.0** | 0 | 0 | |
-0.22 | 9.8 ± 2.1 | 7.7 ± 2.1 | 74.2 ± 5.9 | 0.8 ± 0.5** | 0 | 0 | |
MeA | -1.82 | 158.8 ± 14.7 | 117.0 ± 9.0 | 73.9 ± 1.3 | 15.3 ± 4.0** | 0.7 ± 0.3 | 3.6 ± 1.8 |
-1.94 | 223.0 ± 19.1 | 141.8 ± 16.7 | 63.2 ± 4.8 | 80.8 ± 19.1** | 1.3 ± 1.3 | 1.2 ± 1.2 | |
-2.06 | 198.7 ± 18.4 | 210.0 ± 15.5 | 70.1 ± 1.4 | 96.3 ± 11.5** | 0.7 ± 0.3 | 0.8 ± 0.4 |
Total ERβ cell number, Number of ERβ-immunopositive cells/side in the targeted area; double-labeled cell number, number of double-stained cells with ERβ and GFP; double-labeled % in total, percentage of double-stained cells in the total number of ERβ-immunopositive cells.
*p< 0.05, **p< 0.01 versus Control.