Membrane and action potential properties of male and female nucleus accumbens shell medium spiny neurons
| Property | Male | Female | Statistics (t/U, p) | Data structure | Type of test | 95% confidence interval |
|---|---|---|---|---|---|---|
| Resting potential (mV) | −82.78 ± 1.650 (27) | −86.10 ± 0.8644 (35) | 1.90, 0.06 | Normally distributed | Student's t test | −0.18 to 6.82 |
| Input resistance (MΩ) | 337.6 ± 32.57 (27) | 278.6 ± 18.24 (35) | 397.0, 0.29 | Normality not assumed | Mann–Whitney U test | −98.03 to 24.29 |
| Time constant of the membrane (ms) | 22.53 ± 1.60 (27) | 20.38 ± 1.03 (35) | 1.18, 0.24 | Normally distributed | Student's t test | −1.51 to 5.82 |
| Capacitance (pF) | 70.53 ± 2.547 (27) | 78.28 ± 3.398 (35) | 1.17, 0.09 | Normally distributed | Student's t test | −16.70 to 1.19 |
| Rectified range input resistance (MΩ) | 204.6 ± 16.4 (27) | 169.3 ± 9.5 (35) | 364.0, 0.13 | Normality not assumed | Mann–Whitney U test | −56.6 to 7.7 |
| Inward rectification (MΩ) | 132.9 ± 17.17 (27) | 109.3 ± 9.40 (35) | 426.0, 0.51 | Normality not assumed | Mann–Whitney U test | −43.60 to 19.45 |
| Inward rectification (%) | 62.83 ± 1.46 (27) | 62.53 ± 1.21 (35) | 464.0, 0.91 | Normality not assumed | Mann–Whitney U test | −3.85 to 3.68 |
| Sag index | 0.014 ± 0.002 (27) | 0.014 ± 0.002 (35) | 448.5, 0.74 | Normality not assumed | Mann–Whitney U test | −0.005 to 0.003 |
| AP threshold (mV) | −54.25 ± 0.98 (27) | −55.16 ± 0.66 (35) | 400.0, 0.31 | Normality not assumed | Mann–Whitney U test | −3.17 to 0.85 |
| AP amplitude (mV) | 63.40 ± 1.13 (27) | 61.97 ± 1.62 (35) | 421.0, 0.47 | Normality not assumed | Mann–Whitney U test | −6.59 to 3.50 |
| AP width at half-peak (ms) | 2.14 ± 0.10 (27) | 2.06 ± 0.04 (35) | 444.5, 0.70 | Normality not assumed | Mann–Whitney U test | −0.20 to 0.13 |
| AHP peak (mV) | −8.87 ± 0.53 (27) | −7.61 ± 0.51 (35) | 1.68, 0.10 | Normally distributed | Student's t test | −2.75 to 0.24 |
| AHP time to peak (ms) | 38.05 ± 4.09 (27) | 34.83 ± 1.901 (35) | 458.5, 0.85 | Normality not assumed | Mann–Whitney U test | −7.77 to 5.37 |
| Delay to first spike (ms) | 445.3 ± 16.56 (25) | 455.5 ± 15.89 (27) | 0.44, 0.66 | Normally distributed | Student's t test | −56.35 to 35.95 |
| Rheobase (nA) | 0.059 ± 0.005 (27) | 0.069 ± 0.005 (35) | 0.03, 0.16 | Normally distributed | Student's t test | −0.024 to 0.004 |
| FI slope (Hz/nA) | 356.3 ± 40.55 (27) | 365.7 ± 17.59 (35) | 369.5, 0.15 | Normality not assumed | Mann–Whitney U test | −16.64 to 81.30 |
Values are reported as the mean ± SEM (sample size), unless otherwise indicated. The sag index is unitless. None of these neurons fired spontaneous action potentials. No significant differences were detected. AP, Action potential; AHP, afterhyperpolarization.