Table 2.

Statistical tests utilized in this manuscript

	Data Structure	Type of Test	95% Confidence Interval
a	Non-normally distributed	Mann Whitney Test	−1.982 to 0
b	Normally Distributed	2-way repeated measures ANOVA	BL		−1.786 to 0.9921
			2 Days		1.399 to 4.177
			2 Weeks		1.116 to 3.894
			3 Week		0.6599 to 3.438
			4 Weeks		1.513 to 4.291
			6 Weeks		1.053 to 3.831
			8 Weeks		0.9196 to 3.698
c	Normally Distributed	2-way repeated measures ANOVA	BL		−1.895 to 1.047
			2 Days		−3.114 to −0.1722
			2 Weeks		−3.073 to −0.1315
			3 Weeks		−3.467 to −0.5257
			4 Weeks		−3.824 to −0.8828
			6 Weeks		−3.708 to −0.7663
			8 Weeks		−3.797 to −0.8067
d	Normally Distributed	2-way repeated measures ANOVA	BL		−2.017 to −0.6001
			2 Days		−0.1975 to 1.219
			2 Weeks		0.2870 to 1.704
			3 Weeks		−0.1994 to 1.218
			4 Weeks		−0.1833 to 1.234
			6 Weeks		−0.2278 to 1.189
			8 Weeks		−0.5649 to 0.8520
e	Normally Distributed	2-way repeated measures ANOVA	BL		−6.579 to 70.27
			2 Days		15.50 to 92.35
			2 Weeks		−2.743 to 74.11
			3 Weeks		−0.06598 to 76.78
			4 Weeks		−0.1534 to 76.70
			6 Weeks		−18.63 to 58.22
			8 Weeks		−6.579 to 70.27
f	Normally Distributed	Student’s t-test	−12.04 to 8.812
g	Normally Distributed	2-way repeated measures ANOVA	BL		−19.12 to 9.119
			2 Days		31.71 to 59.95
			2 Weeks		27.96 to 56.20
			3 Weeks		35.05 to 63.29
			4 Weeks		31.30 to 59.54
			6 Weeks		31.30 to 59.54
			8 Weeks		31.30 to 59.54
h	Normally Distributed	2-way repeated measures ANOVA	BL		−30.63 to 19.91
			2 Days		−20.39 to 30.15
			2 Weeks		−14.44 to 36.10
			3 Weeks		7.705 to 58.25
			4 Weeks		15.32 to 65.87
			6 Weeks		11.63 to 62.18
			8 Weeks		10.93 to 62.31
i	Normally Distributed	2-way repeated measures ANOVA	BL		−17.81 to 23.52
			2 Days		1.122 to 42.45
			2 Weeks		8.086 to 49.41
			3 Weeks		−10.31 to 31.02
			4 Weeks		−13.34 to 27.99
			6 Weeks		−10.49 to 30.84
			8 Weeks		−22.63 to 18.70
j	Normally Distributed	1-way ANOVA	0.8595 to 1.648 for Young height
			0.2179 to 1.028 for Aged height
			0.3473 to 1.482 for Young width
			0.4451 to 1.610 for Aged height
k	Normally Distributed	2-way ANOVA	15.00 mN		−2.979 to 1.547
			35.00 mN		−2.442 to 2.084
			70.00 mN		1.031 to 5.556
			140.0 mN		3.124 to 7.650
l	Normally Distributed	2-way ANOVA	15.00 mN		−2.376 to 2.028
			35.00 mN		−2.117 to 2.287
			70.00 mN		−0.5504 to 3.854
			140.0 mN		−0.3346 to 4.101
m	Normally Distributed	2-way ANOVA	15.00 mN		−1.645 to 2.507
			35.00 mN		−1.791 to 2.361
			70.00 mN		−3.398 to 0.7536
			140.0 mN		−5.603 to −1.451
n	Non-Normally Distributed	Kruskal-Wallis Test	Aged CFA − 2 days vs Aged PBS − 2 days		−0.3564 to 0.4208
			Aged CFA − 2 days vs Young CFA − 2 days		−0.3346 to 0.4188
			Aged CFA − 2 days vs Young PBS − 2 days		−0.2396 to 0.5376
			Aged PBS − 2 days vs Young CFA − 2 days		−0.3906 to 0.4105
			Aged PBS − 2 days vs Young PBS − 2 days		−0.2949 to 0.5285
			Young CFA − 2 days vs Young PBS − 2 days		−0.2937 to 0.5074
o	Non-Normally Distributed	Contingency Table followed by Fisher’s Exact Test	1.081 to 10.12 for Aged Odds Ratio
			0.9011 to 8.377 for Young Odds Ratio
			(95% CI cannot be calculated for Fisher’s Exact test alone)
p	Normally Distributed	2-way ANOVA	15.00 mN		−3.588 to 0.02884
			35.00 mN		−3.934 to −0.3170
			70.00 mN		−3.356 to 0.2608
			140.0 mN		−1.688 to 2.081
q	Normally Distributed	2-way ANOVA	15.00 mN		−3.198 to 1.455
			35.00 mN		−3.370 to 1.284
			70.00 mN		−3.109 to 1.544
			140.0 mN		−2.994 to 1.691
r	Normally Distributed	2-way ANOVA	15.00 mN		−1.302 to 2.701
			35.00 mN		−0.3710 to 3.632
			70.00 mN		2.282 to 6.286
			140.0 mN		1.971 to 6.009
s	Normally Distributed	2-way ANOVA	15.00 mN		−2.522 to 1.914
			35.00 mN		−3.274 to 1.162
			70.00 mN		−5.297 to −0.8607
			140.0 mN		−5.677 to −1.170
t	Non-Normally Distributed	Kruskal-Wallis Test	Aging PBS vs CFA		0.1826 to 1.322
		Kruskal-Wallis Test	Young PBS vs CFA		0.4658 to 1.511
u	Non-Normally Distributed	Contingency Table followed by Fisher’s Exact Test	1.590 to 15.73 for Young Odds Ratio
			0.5893 to 5.284 for Aged Odds Ratio
			(95% CI cannot be calculated for Fisher’s Exact test alone)
v	Non-Normally Distributed	Contingency Table followed by Fisher’s Exact Test	1.770 to 2.348 for Young 2-day CFA vs PBS Odds Ratio
			1.597 to 1.995 for Aged 2-day CFA vs PBS Odds Ratio
			(95% CI cannot be calculated for Fisher’s Exact test alone)
w	Normally Distributed	One-way ANOVA	CFA - 8 wk vs PBS - 8 wk		0.05562 to 0.1652
			CFA 2 days vs PBS - 2 days		−0.1246 to −0.01475
			Aged - 8 wk PBS vs Aged 8 wk CFA		−0.06385 to 0.01858
			Aged 2 day CFA vs Aged 2 day PBS		0.03960 to 0.1341
x	Normally Distributed	One-way ANOVA	Aged 8 Wk CFA vs Aged 8 Wk PBS		−0.7127 to 0.9026
			Aged 8 Wk CFA vs Aged 2 day CFA		−0.4550 to 1.054
			Aged 8 Wk CFA vs Aged 2 day PBS		−0.7648 to 0.8323
			Aged 8 Wk CFA vs Young 8 week CFA		−0.5910 to 0.9439
			Aged 8 Wk CFA vs Young 8 week PBS		−1.376 to 0.2588
			Aged 8 Wk CFA vs Young 2 days CFA		−0.4760 to 1.073
			Aged 8 Wk CFA vs Young 2 days PBS		−0.6258 to 0.9712
			Aged 8 Wk PBS vs Aged 2 day CFA		−0.6031 to 1.012
			Aged 8 Wk PBS vs Aged 2 day PBS		−0.9101 to 0.7877
			Aged 8 Wk PBS vs Young 8 week CFA		−0.7382 to 0.9013
			Aged 8 Wk PBS vs Young 8 week PBS		−1.520 to 0.2131
			Aged 8 Wk PBS vs Young 2 days CFA		−0.6228 to 1.030
			Aged 8 Wk PBS vs Young 2 days PBS		−0.7712 to 0.9267
			Aged 2 day CFA vs Aged 2 day PBS		−1.064 to 0.5327
			Aged 2 day CFA vs Young 8 week CFA		−0.8905 to 0.6444
			Aged 2 day CFA vs Young 8 week PBS		−1.676 to −0.04072
			Aged 2 day CFA vs Young 2 days CFA		−0.7755 to 0.7735
			Aged 2 day CFA vs Young 2 days PBS		−0.9253 to 0.6717
			Aged 2 day PBS vs Young 8 week CFA		−0.6680 to 0.9535
			Aged 2 day PBS vs Young 8 week PBS		−1.451 to 0.2658
			Aged 2 day PBS vs Young 2 days CFA		−0.5526 to 1.082
			Aged 2 day PBS vs Young 2 days PBS		−0.7013 to 0.9792
			Young 8 week CFA vs Young 8 week PBS		−1.565 to 0.09424
			Young 8 week CFA vs Young 2 days CFA		−0.6650 to 0.9091
			Young 8 week CFA vs Young 2 days PBS		−0.8145 to 0.8069
			Young 8 week PBS vs Young 2 days CFA		0.02128 to 1.693
			Young 8 week PBS vs Young 2 days PBS		−0.1269 to 1.590
			Young 2 days CFA vs Young 2 days PBS		−0.9432 to 0.6916
y	Normally Distributed	Chi Square followed by Fisher’s exact test	1.342 to 24.34 for naive vs 8-week CFA Odds Ratio
			1.111 to 21.82 for 2-day CFA vs 8-week CFA Odds Ratio
			(95% CI cannot be calculated for Fisher’s Exact test alone)
z	Normally Distributed	1-way ANOVA	Naive vs 2-day CFA		−31.44 to 46.29
			Naive vs 8-week CFA		−21.96 to 85.90
			2-day CFA vs 8-week CFA		−30.92 to 80.01
aa	Normally Distributed	Chi Square	0.2197 to 31.37 for naïve vs 2-day Odds Ratio
			0.03641 to 6.866 for 2-day vs 8-week Odds Ratio
			0.1677 to 10.27 for naïve vs 8-week Odds Ratio
			(95% CI cannot be calculated for Fisher’s Exact test alone)
bb	Normally Distributed	1-way ANOVA	Naive vs 2-day		−99.31 to 0.3113
			Naive vs 8-week		−90.56 to 9.061
			2-day vs 8-week		−41.06 to 58.56
cc	Normally Distributed	2-way ANOVA	Naive vs 2-day
			Force
			15.00		−8.125 to 1.035
			35.00		−7.587 to 1.573
			70.00		−7.013 to 2.147
			140.0		−6.602 to 2.701
			Naive vs 8 week
			Force		−7.934 to 2.113
			15.00		−8.314 to 1.732
			35.00		−8.317 to 1.729
			70.00		−7.230 to 2.816
			140.0		−7.934 to 2.113
			2-day vs 8 week
			Force
			15.00		−4.665 to 5.935
			35.00		−5.584 to 5.017
			70.00		−6.161 to 4.440
			140.0		−5.619 to 5.106
dd	Non-Normally Distributed	Kruskall Wallis Test		Naïve	2-day	8-week
			Lower 95% CI	0.4476	0.7617	0.9286
			Upper 95% CI	0.8724	1.083	1.929
ee	Normally Distributed	Student’s t-test for PBS vs CFA for each time point and gene	−0.9854 to −0.1312 Young 2-day Nav1.7
			−1.848 to 0.07474 Young 2-day Nav1.8
			−1.643 to 0.4202 Young 2-day Nav1.9
			−1.886 to 0.2060 Young 2-day Piezo2
			−2.157 to −0.7731 Young 2-day TRPA1
			−1.294 to 0.06031 Young 2-day TRPV1
			−1.014 to 0.1110 Young 2-day TRPC3
			0.007134 to 1.246 Young 2-day TRPC6
			−1.581 to 0.6142 Young 2-day Kv1.1
			−1.399 to 0.07587 Young 2-day Kv7.2
			−1.710 to −0.06359 Young 2-day Kv7.3
			−0.6277 to 0.7177 Young 8-week Nav1.7
			−0.9606 to 0.4572 Young 8-week Nav1.8
			−0.8359 to 0.6292 Young 8-week Nav1.9
			−1.141 to 0.8441 Young 8-week Piezo2
			−0.7882 to 0.4815 Young 8-week TRPA1
			−0.9529 to 0.4529 Young 8-week TRPV1
			−1.469 to 0.8719 Young 8-week TRPC3
			−0.5417 to 1.498 Young 8-week TRPC6
			−0.7716 to 0.1383 Young 8-week Kv1.1
			−1.292 to 0.4155 Young 8-week Kv7.2
			−1.370 to 0.3438 Young 8-week Kv7.3
			−0.6742 to 0.5142 Aged 2-day Nav1.7
			−0.9797 to 0.9464 Aged 2-day Nav1.8
			−0.9318 to 1.192 Aged 2-day Nav1.9
			−0.8022 to 0.4022 Aged 2-day Piezo2
			−2.579 to −0.4873 Aged 2-day TRPA1
			−1.068 to 0.08780 Aged 2-day TRPV1
			0.02838 to 0.9250 Aged 2-day TRPC3
			−0.5024 to 1.082 Aged 2-day TRPC6
			−0.07159 to 0.5116 Aged 2-day Kv1.1
			−0.4090 to 0.6290 Aged 2-day Kv7.2
			−0.4557 to 0.5357 Aged 2-day Kv7.3
			−0.3108 to 0.5842 Aged 8-week Nav1.7
			−0.7250 to 0.02833 Aged 8-week Nav1.8
			−0.6878 to 0.1078 Aged 8-week Nav1.9
			−0.7805 to 0.3605 Aged 8-week Piezo2
			−1.981 to −0.4358 Aged 8-week TRPA1
			−0.7546 to −0.03539 Aged 8-week TRPV1
			−0.6783 to 0.8150 Aged 8-week TRPC3
			−1.052 to 0.8521 Aged 8-week TRPC6
			−0.1824 to 0.8290 Aged 8 week Kv1.1
			−0.5183 to 0.6183 Aged 8 week Kv7.2
			−0.4955 to 0.6255 Aged 8 week Kv7.3
ff	Normally Distributed	One-way ANOVA	Nav1.7
			Aged 2-day vs Aged 8 weeks		−0.2611 to 0.5441
			Aged 2-day vs Young 2-day		−0.8386 to −0.03346
			Aged 8 weeks vs Young 8 week		−0.3855 to 0.2719
			Young 2-day vs Young 8 week		0.1920 to 0.8494
			Nav1.8
			Aged 2-day vs Aged 8 weeks		−1.284 to 0.7893
			Aged 2-day vs Young 2-day		−2.104 to −0.03105
			Aged 8 weeks vs Young 8 week		−0.7631 to 0.9298
			Young 2-day vs Young 8 week		0.05728 to 1.750
			Nav1.9
			Aged 2-day vs Aged 8 weeks		−1.233 to 0.6500
			Aged 2-day vs Young 2-day		−1.694 to 0.1896
			Aged 8 weeks vs Young 8 week		−0.6290 to 0.9088
			Young 2-day vs Young 8 week		−0.1685 to 1.369
			Piezo2
			Aged 2-day vs Aged 8 weeks		−1.071 to 1.067
			Aged 2-day vs Young 2-day		−1.880 to 0.2578
			Aged 8 weeks vs Young 8 week		−0.8390 to 0.9063
			Young 2-day vs Young 8 week		−0.03008 to 1.715
			TRPA1
			Aged 2-day vs Aged 8 weeks		−0.8925 to 2.265
			Aged 2-day vs Young 2-day		−1.406 to 1.752
			Aged 8 weeks vs Young 8 week		−0.04212 to 2.536
			Young 2-day vs Young 8 week		−0.4714 to 3.050
			TRPV1
			Aged 2-day vs Aged 8 weeks		−0.6561 to 0.7992
			Aged 2-day vs Young 2-day		−0.9202 to 0.5352
			Aged 8 weeks vs Young 8 week		−0.4682 to 0.7201
			Young 2-day vs Young 8 week		−0.2041 to 0.9841
			TRPC3
			Aged 2-day vs Aged 8 weeks		−1.036 to 0.4285
			Aged 2-day vs Young 2-day		−1.465 to −0.0002279
			Aged 8 weeks vs Young 8 week		−0.8156 to 0.3804
			Young 2-day vs Young 8 week		−0.3868 to 0.8092
			TRPC6
			Aged 2-day vs Aged 8 weeks		−0.3808 to 0.8767
			Aged 2-day vs Young 2-day		−0.3464 to 0.9110
			Aged 8 weeks vs Young 8 week		−0.3308 to 0.6959
			Young 2-day vs Young 8 week		−0.3651 to 0.6616
			Kv1.1
			Aged 2-day vs Aged 8 weeks		−0.9476 to 1.019
			Aged 2-day vs Young 2-day		−1.693 to 0.2736
			Aged 8 weeks vs Young 8 week		−1.234 to 0.3716
			Young 2-day vs Young 8 week		−0.4887 to 1.117
			Kv7.2
			Aged 2-day vs Aged 8 weeks		−0.9518 to 0.8372
			Aged 2-day vs Young 2-day		−1.666 to 0.1225
			Aged 8 weeks vs Young 8 week		−1.099 to 0.3614
			Young 2-day vs Young 8 week		−0.3847 to 1.076
			Kv7.3
			Aged 2-day vs Aged 8 weeks		−1.049 to 1.010
			Aged 2-day vs Young 2-day		−2.029 to 0.02973
			Aged 8 weeks vs Young 8 week		−1.274 to 0.4071
			Young 2-day vs Young 8 week		−0.2940 to 1.387