Statistical tests utilized in this manuscript
Data Structure | Type of Test | 95% Confidence Interval | ||||
---|---|---|---|---|---|---|
a | Non-normally distributed | Mann Whitney Test | −1.982 to 0 | |||
b | Normally Distributed | 2-way repeated measures ANOVA | BL | −1.786 to 0.9921 | ||
2 Days | 1.399 to 4.177 | |||||
2 Weeks | 1.116 to 3.894 | |||||
3 Week | 0.6599 to 3.438 | |||||
4 Weeks | 1.513 to 4.291 | |||||
6 Weeks | 1.053 to 3.831 | |||||
8 Weeks | 0.9196 to 3.698 | |||||
c | Normally Distributed | 2-way repeated measures ANOVA | BL | −1.895 to 1.047 | ||
2 Days | −3.114 to −0.1722 | |||||
2 Weeks | −3.073 to −0.1315 | |||||
3 Weeks | −3.467 to −0.5257 | |||||
4 Weeks | −3.824 to −0.8828 | |||||
6 Weeks | −3.708 to −0.7663 | |||||
8 Weeks | −3.797 to −0.8067 | |||||
d | Normally Distributed | 2-way repeated measures ANOVA | BL | −2.017 to −0.6001 | ||
2 Days | −0.1975 to 1.219 | |||||
2 Weeks | 0.2870 to 1.704 | |||||
3 Weeks | −0.1994 to 1.218 | |||||
4 Weeks | −0.1833 to 1.234 | |||||
6 Weeks | −0.2278 to 1.189 | |||||
8 Weeks | −0.5649 to 0.8520 | |||||
e | Normally Distributed | 2-way repeated measures ANOVA | BL | −6.579 to 70.27 | ||
2 Days | 15.50 to 92.35 | |||||
2 Weeks | −2.743 to 74.11 | |||||
3 Weeks | −0.06598 to 76.78 | |||||
4 Weeks | −0.1534 to 76.70 | |||||
6 Weeks | −18.63 to 58.22 | |||||
8 Weeks | −6.579 to 70.27 | |||||
f | Normally Distributed | Student’s t-test | −12.04 to 8.812 | |||
g | Normally Distributed | 2-way repeated measures ANOVA | BL | −19.12 to 9.119 | ||
2 Days | 31.71 to 59.95 | |||||
2 Weeks | 27.96 to 56.20 | |||||
3 Weeks | 35.05 to 63.29 | |||||
4 Weeks | 31.30 to 59.54 | |||||
6 Weeks | 31.30 to 59.54 | |||||
8 Weeks | 31.30 to 59.54 | |||||
h | Normally Distributed | 2-way repeated measures ANOVA | BL | −30.63 to 19.91 | ||
2 Days | −20.39 to 30.15 | |||||
2 Weeks | −14.44 to 36.10 | |||||
3 Weeks | 7.705 to 58.25 | |||||
4 Weeks | 15.32 to 65.87 | |||||
6 Weeks | 11.63 to 62.18 | |||||
8 Weeks | 10.93 to 62.31 | |||||
i | Normally Distributed | 2-way repeated measures ANOVA | BL | −17.81 to 23.52 | ||
2 Days | 1.122 to 42.45 | |||||
2 Weeks | 8.086 to 49.41 | |||||
3 Weeks | −10.31 to 31.02 | |||||
4 Weeks | −13.34 to 27.99 | |||||
6 Weeks | −10.49 to 30.84 | |||||
8 Weeks | −22.63 to 18.70 | |||||
j | Normally Distributed | 1-way ANOVA | 0.8595 to 1.648 for Young height | |||
0.2179 to 1.028 for Aged height | ||||||
0.3473 to 1.482 for Young width | ||||||
0.4451 to 1.610 for Aged height | ||||||
k | Normally Distributed | 2-way ANOVA | 15.00 mN | −2.979 to 1.547 | ||
35.00 mN | −2.442 to 2.084 | |||||
70.00 mN | 1.031 to 5.556 | |||||
140.0 mN | 3.124 to 7.650 | |||||
l | Normally Distributed | 2-way ANOVA | 15.00 mN | −2.376 to 2.028 | ||
35.00 mN | −2.117 to 2.287 | |||||
70.00 mN | −0.5504 to 3.854 | |||||
140.0 mN | −0.3346 to 4.101 | |||||
m | Normally Distributed | 2-way ANOVA | 15.00 mN | −1.645 to 2.507 | ||
35.00 mN | −1.791 to 2.361 | |||||
70.00 mN | −3.398 to 0.7536 | |||||
140.0 mN | −5.603 to −1.451 | |||||
n | Non-Normally Distributed | Kruskal-Wallis Test | Aged CFA − 2 days vs Aged PBS − 2 days | −0.3564 to 0.4208 | ||
Aged CFA − 2 days vs Young CFA − 2 days | −0.3346 to 0.4188 | |||||
Aged CFA − 2 days vs Young PBS − 2 days | −0.2396 to 0.5376 | |||||
Aged PBS − 2 days vs Young CFA − 2 days | −0.3906 to 0.4105 | |||||
Aged PBS − 2 days vs Young PBS − 2 days | −0.2949 to 0.5285 | |||||
Young CFA − 2 days vs Young PBS − 2 days | −0.2937 to 0.5074 | |||||
o | Non-Normally Distributed | Contingency Table followed by Fisher’s Exact Test | 1.081 to 10.12 for Aged Odds Ratio | |||
0.9011 to 8.377 for Young Odds Ratio | ||||||
(95% CI cannot be calculated for Fisher’s Exact test alone) | ||||||
p | Normally Distributed | 2-way ANOVA | 15.00 mN | −3.588 to 0.02884 | ||
35.00 mN | −3.934 to −0.3170 | |||||
70.00 mN | −3.356 to 0.2608 | |||||
140.0 mN | −1.688 to 2.081 | |||||
q | Normally Distributed | 2-way ANOVA | 15.00 mN | −3.198 to 1.455 | ||
35.00 mN | −3.370 to 1.284 | |||||
70.00 mN | −3.109 to 1.544 | |||||
140.0 mN | −2.994 to 1.691 | |||||
r | Normally Distributed | 2-way ANOVA | 15.00 mN | −1.302 to 2.701 | ||
35.00 mN | −0.3710 to 3.632 | |||||
70.00 mN | 2.282 to 6.286 | |||||
140.0 mN | 1.971 to 6.009 | |||||
s | Normally Distributed | 2-way ANOVA | 15.00 mN | −2.522 to 1.914 | ||
35.00 mN | −3.274 to 1.162 | |||||
70.00 mN | −5.297 to −0.8607 | |||||
140.0 mN | −5.677 to −1.170 | |||||
t | Non-Normally Distributed | Kruskal-Wallis Test | Aging PBS vs CFA | 0.1826 to 1.322 | ||
Young PBS vs CFA | 0.4658 to 1.511 | |||||
u | Non-Normally Distributed | Contingency Table followed by Fisher’s Exact Test | 1.590 to 15.73 for Young Odds Ratio | |||
0.5893 to 5.284 for Aged Odds Ratio | ||||||
(95% CI cannot be calculated for Fisher’s Exact test alone) | ||||||
v | Non-Normally Distributed | Contingency Table followed by Fisher’s Exact Test | 1.770 to 2.348 for Young 2-day CFA vs PBS Odds Ratio | |||
1.597 to 1.995 for Aged 2-day CFA vs PBS Odds Ratio | ||||||
(95% CI cannot be calculated for Fisher’s Exact test alone) | ||||||
w | Normally Distributed | One-way ANOVA | CFA - 8 wk vs PBS - 8 wk | 0.05562 to 0.1652 | ||
CFA 2 days vs PBS - 2 days | −0.1246 to −0.01475 | |||||
Aged - 8 wk PBS vs Aged 8 wk CFA | −0.06385 to 0.01858 | |||||
Aged 2 day CFA vs Aged 2 day PBS | 0.03960 to 0.1341 | |||||
x | Normally Distributed | One-way ANOVA | Aged 8 Wk CFA vs Aged 8 Wk PBS | −0.7127 to 0.9026 | ||
Aged 8 Wk CFA vs Aged 2 day CFA | −0.4550 to 1.054 | |||||
Aged 8 Wk CFA vs Aged 2 day PBS | −0.7648 to 0.8323 | |||||
Aged 8 Wk CFA vs Young 8 week CFA | −0.5910 to 0.9439 | |||||
Aged 8 Wk CFA vs Young 8 week PBS | −1.376 to 0.2588 | |||||
Aged 8 Wk CFA vs Young 2 days CFA | −0.4760 to 1.073 | |||||
Aged 8 Wk CFA vs Young 2 days PBS | −0.6258 to 0.9712 | |||||
Aged 8 Wk PBS vs Aged 2 day CFA | −0.6031 to 1.012 | |||||
Aged 8 Wk PBS vs Aged 2 day PBS | −0.9101 to 0.7877 | |||||
Aged 8 Wk PBS vs Young 8 week CFA | −0.7382 to 0.9013 | |||||
Aged 8 Wk PBS vs Young 8 week PBS | −1.520 to 0.2131 | |||||
Aged 8 Wk PBS vs Young 2 days CFA | −0.6228 to 1.030 | |||||
Aged 8 Wk PBS vs Young 2 days PBS | −0.7712 to 0.9267 | |||||
Aged 2 day CFA vs Aged 2 day PBS | −1.064 to 0.5327 | |||||
Aged 2 day CFA vs Young 8 week CFA | −0.8905 to 0.6444 | |||||
Aged 2 day CFA vs Young 8 week PBS | −1.676 to −0.04072 | |||||
Aged 2 day CFA vs Young 2 days CFA | −0.7755 to 0.7735 | |||||
Aged 2 day CFA vs Young 2 days PBS | −0.9253 to 0.6717 | |||||
Aged 2 day PBS vs Young 8 week CFA | −0.6680 to 0.9535 | |||||
Aged 2 day PBS vs Young 8 week PBS | −1.451 to 0.2658 | |||||
Aged 2 day PBS vs Young 2 days CFA | −0.5526 to 1.082 | |||||
Aged 2 day PBS vs Young 2 days PBS | −0.7013 to 0.9792 | |||||
Young 8 week CFA vs Young 8 week PBS | −1.565 to 0.09424 | |||||
Young 8 week CFA vs Young 2 days CFA | −0.6650 to 0.9091 | |||||
Young 8 week CFA vs Young 2 days PBS | −0.8145 to 0.8069 | |||||
Young 8 week PBS vs Young 2 days CFA | 0.02128 to 1.693 | |||||
Young 8 week PBS vs Young 2 days PBS | −0.1269 to 1.590 | |||||
Young 2 days CFA vs Young 2 days PBS | −0.9432 to 0.6916 | |||||
y | Normally Distributed | Chi Square followed by Fisher’s exact test | 1.342 to 24.34 for naive vs 8-week CFA Odds Ratio | |||
1.111 to 21.82 for 2-day CFA vs 8-week CFA Odds Ratio | ||||||
(95% CI cannot be calculated for Fisher’s Exact test alone) | ||||||
z | Normally Distributed | 1-way ANOVA | Naive vs 2-day CFA | −31.44 to 46.29 | ||
Naive vs 8-week CFA | −21.96 to 85.90 | |||||
2-day CFA vs 8-week CFA | −30.92 to 80.01 | |||||
aa | Normally Distributed | Chi Square | 0.2197 to 31.37 for naïve vs 2-day Odds Ratio | |||
0.03641 to 6.866 for 2-day vs 8-week Odds Ratio | ||||||
0.1677 to 10.27 for naïve vs 8-week Odds Ratio | ||||||
(95% CI cannot be calculated for Fisher’s Exact test alone) | ||||||
bb | Normally Distributed | 1-way ANOVA | Naive vs 2-day | −99.31 to 0.3113 | ||
Naive vs 8-week | −90.56 to 9.061 | |||||
2-day vs 8-week | −41.06 to 58.56 | |||||
cc | Normally Distributed | 2-way ANOVA | Naive vs 2-day | |||
Force | ||||||
15.00 | −8.125 to 1.035 | |||||
35.00 | −7.587 to 1.573 | |||||
70.00 | −7.013 to 2.147 | |||||
140.0 | −6.602 to 2.701 | |||||
Naive vs 8 week | ||||||
Force | −7.934 to 2.113 | |||||
15.00 | −8.314 to 1.732 | |||||
35.00 | −8.317 to 1.729 | |||||
70.00 | −7.230 to 2.816 | |||||
140.0 | −7.934 to 2.113 | |||||
2-day vs 8 week | ||||||
Force | ||||||
15.00 | −4.665 to 5.935 | |||||
35.00 | −5.584 to 5.017 | |||||
70.00 | −6.161 to 4.440 | |||||
140.0 | −5.619 to 5.106 | |||||
dd | Non-Normally Distributed | Kruskall Wallis Test | Naïve | 2-day | 8-week | |
Lower 95% CI | 0.4476 | 0.7617 | 0.9286 | |||
Upper 95% CI | 0.8724 | 1.083 | 1.929 | |||
ee | Normally Distributed | Student’s t-test for PBS vs CFA for each time point and gene | −0.9854 to −0.1312 Young 2-day Nav1.7 | |||
−1.848 to 0.07474 Young 2-day Nav1.8 | ||||||
−1.643 to 0.4202 Young 2-day Nav1.9 | ||||||
−1.886 to 0.2060 Young 2-day Piezo2 | ||||||
−2.157 to −0.7731 Young 2-day TRPA1 | ||||||
−1.294 to 0.06031 Young 2-day TRPV1 | ||||||
−1.014 to 0.1110 Young 2-day TRPC3 | ||||||
0.007134 to 1.246 Young 2-day TRPC6 | ||||||
−1.581 to 0.6142 Young 2-day Kv1.1 | ||||||
−1.399 to 0.07587 Young 2-day Kv7.2 | ||||||
−1.710 to −0.06359 Young 2-day Kv7.3 | ||||||
−0.6277 to 0.7177 Young 8-week Nav1.7 | ||||||
−0.9606 to 0.4572 Young 8-week Nav1.8 | ||||||
−0.8359 to 0.6292 Young 8-week Nav1.9 | ||||||
−1.141 to 0.8441 Young 8-week Piezo2 | ||||||
−0.7882 to 0.4815 Young 8-week TRPA1 | ||||||
−0.9529 to 0.4529 Young 8-week TRPV1 | ||||||
−1.469 to 0.8719 Young 8-week TRPC3 | ||||||
−0.5417 to 1.498 Young 8-week TRPC6 | ||||||
−0.7716 to 0.1383 Young 8-week Kv1.1 | ||||||
−1.292 to 0.4155 Young 8-week Kv7.2 | ||||||
−1.370 to 0.3438 Young 8-week Kv7.3 | ||||||
−0.6742 to 0.5142 Aged 2-day Nav1.7 | ||||||
−0.9797 to 0.9464 Aged 2-day Nav1.8 | ||||||
−0.9318 to 1.192 Aged 2-day Nav1.9 | ||||||
−0.8022 to 0.4022 Aged 2-day Piezo2 | ||||||
−2.579 to −0.4873 Aged 2-day TRPA1 | ||||||
−1.068 to 0.08780 Aged 2-day TRPV1 | ||||||
0.02838 to 0.9250 Aged 2-day TRPC3 | ||||||
−0.5024 to 1.082 Aged 2-day TRPC6 | ||||||
−0.07159 to 0.5116 Aged 2-day Kv1.1 | ||||||
−0.4090 to 0.6290 Aged 2-day Kv7.2 | ||||||
−0.4557 to 0.5357 Aged 2-day Kv7.3 | ||||||
−0.3108 to 0.5842 Aged 8-week Nav1.7 | ||||||
−0.7250 to 0.02833 Aged 8-week Nav1.8 | ||||||
−0.6878 to 0.1078 Aged 8-week Nav1.9 | ||||||
−0.7805 to 0.3605 Aged 8-week Piezo2 | ||||||
−1.981 to −0.4358 Aged 8-week TRPA1 | ||||||
−0.7546 to −0.03539 Aged 8-week TRPV1 | ||||||
−0.6783 to 0.8150 Aged 8-week TRPC3 | ||||||
−1.052 to 0.8521 Aged 8-week TRPC6 | ||||||
−0.1824 to 0.8290 Aged 8 week Kv1.1 | ||||||
−0.5183 to 0.6183 Aged 8 week Kv7.2 | ||||||
−0.4955 to 0.6255 Aged 8 week Kv7.3 | ||||||
ff | Normally Distributed | One-way ANOVA | Nav1.7 | |||
Aged 2-day vs Aged 8 weeks | −0.2611 to 0.5441 | |||||
Aged 2-day vs Young 2-day | −0.8386 to −0.03346 | |||||
Aged 8 weeks vs Young 8 week | −0.3855 to 0.2719 | |||||
Young 2-day vs Young 8 week | 0.1920 to 0.8494 | |||||
Nav1.8 | ||||||
Aged 2-day vs Aged 8 weeks | −1.284 to 0.7893 | |||||
Aged 2-day vs Young 2-day | −2.104 to −0.03105 | |||||
Aged 8 weeks vs Young 8 week | −0.7631 to 0.9298 | |||||
Young 2-day vs Young 8 week | 0.05728 to 1.750 | |||||
Nav1.9 | ||||||
Aged 2-day vs Aged 8 weeks | −1.233 to 0.6500 | |||||
Aged 2-day vs Young 2-day | −1.694 to 0.1896 | |||||
Aged 8 weeks vs Young 8 week | −0.6290 to 0.9088 | |||||
Young 2-day vs Young 8 week | −0.1685 to 1.369 | |||||
Piezo2 | ||||||
Aged 2-day vs Aged 8 weeks | −1.071 to 1.067 | |||||
Aged 2-day vs Young 2-day | −1.880 to 0.2578 | |||||
Aged 8 weeks vs Young 8 week | −0.8390 to 0.9063 | |||||
Young 2-day vs Young 8 week | −0.03008 to 1.715 | |||||
TRPA1 | ||||||
Aged 2-day vs Aged 8 weeks | −0.8925 to 2.265 | |||||
Aged 2-day vs Young 2-day | −1.406 to 1.752 | |||||
Aged 8 weeks vs Young 8 week | −0.04212 to 2.536 | |||||
Young 2-day vs Young 8 week | −0.4714 to 3.050 | |||||
TRPV1 | ||||||
Aged 2-day vs Aged 8 weeks | −0.6561 to 0.7992 | |||||
Aged 2-day vs Young 2-day | −0.9202 to 0.5352 | |||||
Aged 8 weeks vs Young 8 week | −0.4682 to 0.7201 | |||||
Young 2-day vs Young 8 week | −0.2041 to 0.9841 | |||||
TRPC3 | ||||||
Aged 2-day vs Aged 8 weeks | −1.036 to 0.4285 | |||||
Aged 2-day vs Young 2-day | −1.465 to −0.0002279 | |||||
Aged 8 weeks vs Young 8 week | −0.8156 to 0.3804 | |||||
Young 2-day vs Young 8 week | −0.3868 to 0.8092 | |||||
TRPC6 | ||||||
Aged 2-day vs Aged 8 weeks | −0.3808 to 0.8767 | |||||
Aged 2-day vs Young 2-day | −0.3464 to 0.9110 | |||||
Aged 8 weeks vs Young 8 week | −0.3308 to 0.6959 | |||||
Young 2-day vs Young 8 week | −0.3651 to 0.6616 | |||||
Kv1.1 | ||||||
Aged 2-day vs Aged 8 weeks | −0.9476 to 1.019 | |||||
Aged 2-day vs Young 2-day | −1.693 to 0.2736 | |||||
Aged 8 weeks vs Young 8 week | −1.234 to 0.3716 | |||||
Young 2-day vs Young 8 week | −0.4887 to 1.117 | |||||
Kv7.2 | ||||||
Aged 2-day vs Aged 8 weeks | −0.9518 to 0.8372 | |||||
Aged 2-day vs Young 2-day | −1.666 to 0.1225 | |||||
Aged 8 weeks vs Young 8 week | −1.099 to 0.3614 | |||||
Young 2-day vs Young 8 week | −0.3847 to 1.076 | |||||
Kv7.3 | ||||||
Aged 2-day vs Aged 8 weeks | −1.049 to 1.010 | |||||
Aged 2-day vs Young 2-day | −2.029 to 0.02973 | |||||
Aged 8 weeks vs Young 8 week | −1.274 to 0.4071 | |||||
Young 2-day vs Young 8 week | −0.2940 to 1.387 |