Percentage of NR islands labeled for PSD proteins and the ratios of labeling intensity at islands to that at PSDs
| Antibodies | Percentage of islands labeleda (number of exp, number of islands scored) | Ratio of labeling intensityb |
|---|---|---|
| NR2B | 97.5 ± 2.5 (4 exp, 42) | 1.76 ± 0.13 (3 exp) |
| GluR2 | 36.3 ± 0.6 (2 exp, 33) | 1.07 ± 0.24 (2 exp) |
| SAP102 | 44.7 ± 4.8 (6 exp, 113) | 1.02 ± 0.15 (4 exp) |
| PSD-95 | 18.3 ± 4.8 (3 exp, 46) | 0.39 ± 0.08 (3 exp) |
| GKAP | 55.7 ± 15.9 (3 exp, 38) | 0.70 ± 0.15 (3 exp) |
| Shank2 | 67.5 ± 12.5 (2 exp, 44) | 0.60 ± 0.12 (2 exp) |
| Homer1 | 63.8 ± 6.4 (5 exp, 105) | 0.59 ± 0.06 (3 exp) |
| CaMKII | 100 (5 exp, 48) | 1.0 ± 0.14 (3 exp) |
Data are reported as the mean ± SEM.
a Percentage of islands labeled at neuronal somas in 3-week-old dissociated hippocampal cultures after depolarization with high K+. Islands were first identified by the structural characteristic of the cytoplasmic density, and then scored for presence of immunogold labeling with the various antibodies.
b Ratio of labeling intensities for each antibody as the number of labels per running micrometer of island divided by number of labels per running micrometer of PSDs in the same experiment.