Summary of pair comparison tests*
Developmental profile of microglia and phagocytic cups | |||||||||||||
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P7 | P10 | P12 | P14 | P17 | P21 | ||||||||
a | Total microglia | compared with P5 | t(6) = 4.534 | t(6) = 4.534 d = 3.70 | t(6) = 1.277 | t(6) = 1.782 | t(6) = 4.534 d = 3.70 | t(6) = 4.534 d = 3.70 | |||||
b | Amoeboid microglia | compared with P5 | t(6) = 1.330 | t(6) = 2.621 d = 2.14 | t(6) = 3.845 d = 3.13 | t(6) = 4.045 d = 3.30 | t(6) = 4.043 d = 3.30 | t(6) = 4.044 d = 3.30 | |||||
c | Stout microglia | compared with P5 | t(6) = 0.395 | t(6) = 1.886 | t(6) = 6.416 d = 5.23 | t(6) = 7.634 d = 6.23 | t(6) = 8.654 d = 7.06 | t(6) = 8.373 d = 6.83 | |||||
d | Microglia with thick processes | compared with P5 | t(6) = 2.659 d = 2.17 | t(6) = 7.858 d = 6.41 | t(6) = 8.657 d = 7.06 | t(6) = 5.344 d = 4.36 | t(6) = 1.926 | t(6) = 1.574 | |||||
e | Microglia with thin processes | compared with P5 | t(6) = 1.000 | t(6) = 5.078 d = 4.14 | t(6) = 3.352 d = 2.73 | t(6) = 4.653 d = 3.79 | t(6) = 25.466 d = 20.79 | t(6) = 31.079 d = 25.37 | |||||
f | Phagocytic cup | compared with P17 | P5 t(6) = 29.336 d = 23.95 | P7 t(6) = 26.068 d = 21.28 | P10 t(6) = 24.687 d = 20.15 | P12 t(6) = 20.255 d = 16.53 | P14 t(6) = 11.571 d = 9.44 | P21 t(6) = 15.762 d = 12.86 | |||||
Microglia and phagocytic cups in the ML and GL during postnatal development | |||||||||||||
g | Total microglia | compared with ML | GL t(10) = 3.356 d = 2.12 | GL t(10) = 2.640 d = 1.66 | GL t(10) = 3.160 d = 1.99 | GL t(10) = 7.438 d = 4.70 | |||||||
h | Amoeboid microglia | compared with ML | GL t(10) = 1.226 | GL t(10) = 0.049 | GL t(10) = 1.000 | GL t(10) = 0.139 | |||||||
i | Stoutmicroglia | compared with ML | GL t(10) = 5.209 d = 3.29 | GL t(10) = 1.192 | GL t(10) = 3.354 d = 2.12 | GL t(10) = 8.846 d = 5.59 | |||||||
j | Microglia with thick processes | compared with ML | GL t(10) = 1.703 | GL t(10) = 1.326 | GL t(10) = 0.601 | GL t(10) = 1.003 | |||||||
k | Microglia with thin processes | compared with ML | GL t(10) = 0.604 | GL t(10) = 0.116 | GL t(10) = 2.191 d = 1.38 | GL t(10) = 6.186 d = 3.91 | |||||||
l | Phagocytic cup | compared with ML | GL t(10) = 4.013 d = 2.53 | GL t(10) = 2.400 d = 1.51 | GL t(10) = 6.814 d = 4.30 | GL t(10) = 2.279 d = 1.44 | |||||||
Microglial phagocytic activity during the third postnatal week of development | |||||||||||||
P15 | P16 | P18 | P19 | ||||||||||
m | Phagocytic cup | compared with GL P17 | GL t(10) = 3.978 d = 2.51 | GL t(10) = 2.198 d = 1.39 | GL t(10) = 0.568 | GL t(10) = 0.518 | |||||||
m | Phagocytic cup | compared with ML P17 | ML t(10) = 1.499 | ML t(10) = 0.029 | ML t(10) = 1.300 | ML t(10) = 2.313 d = 1.46 | |||||||
P15 | P16 | P17 | P18 | P19 | |||||||||
m | Phagocytic cup | compared with the ML | GL t(10) = 0.757 | GL t(10) = 3.052 d = 1.93 | GL t(10) = 4.091 d = 2.58 | GL t(10) = 7.765 d = 4.91 | GL t(10) = 3.348 d = 2.11 | ||||||
Phagocytic cup size during the third postnatal week of development | |||||||||||||
P10 | P14 | P21 | |||||||||||
n | Phagocytic cup | compared with P17 | t(10) = 2.037 d = 1.28 | t(14) = 5.262 d = 2.81 | t(14) = 3.547 d = 1.73 | ||||||||
Pyknotic bodies in the GL than the ML during postnatal development | |||||||||||||
P5 | P14 | P17 | P21 | ||||||||||
p | Pyknotic bodies | compared with GL P7 | GL t(8) = 0.328 | GL t(8) = 34.179 d = 24.16 | GL t(8) = 25.394 d = 17.95 | GL t(8) = 39.642 d = 28.03 | |||||||
p | Pyknotic bodies | compared with ML P7 | ML t(8) = 1.401 | ML t(8) = 0.416 | ML t(8) = 0.729 | ML t(8) = 0.939 | |||||||
P5 | P7 | P14 | P17 | P21 | |||||||||
p | Pyknotic bodies | compared with ML | GL t(10) = 7.163 d = 4.53 | GL t(6) = 19.461 d = 15.88 | GL t(10) = 1.628 | GL t(10) = 0.100 | GL t(10) = 1.392 | ||||||
Sex differences in microglia morphology on P17 cerebellum | |||||||||||||
GL | ML | ||||||||||||
q/u | Amoeboid microglia | compared with females | males t(4) = 0.000 | males t(4) = 1.000 | |||||||||
r/v | Stout microglia | compared with females | males t(4) = 1.278 | males t(4) = 0.496 | |||||||||
s/w | Microglia with thick processes | compared with females | males t(4) = 1.920 | males t(4) = 1.683 | |||||||||
t/x | Microglia with thin processes | compared with females | males t(4) = 3.445 d = 3.445 | males t(4) = 1.858 | |||||||||
y/z | Phagocytic cup | compared with females | males t(4) = 1.044 | males t(4) = 1.726 | |||||||||
aa/bb | Total microglia | compared with females | males t(4) = 0.892 | males t(4) = 1.323 |