Quantification of day of onset, clinical score, and paralysis in EAE experiments
Experiment | Genotype | Mice, n | Day of onset (95% CI) | p | Maximum clinical score(95% CI) | p | Paralysis, % | p |
---|---|---|---|---|---|---|---|---|
1 | lysM-Cre:HIF-1αfl/fl | 17 | 11.7 (11.0–12.5) | 0.77f | 2.71 (2.43–2.98) | 0.77g | 23.5 | 0.73h |
HIF-1αfl/fl | 22 | 11.4 (11.0–12.0) | 2.79 (2.54–3.02) | 31.8 | ||||
2 | lysM-Cre:VHLfl/fl | 20 | 7.95 (7.0–9.5) | 0.90i | 2.95 (2.32–3.52) | 0.90j | 50 | 0.99k |
VHLfl/fl | 15 | 8.56 (7.0–10.0) | 2.82 (2.15–3.47) | 46.7 | ||||
3 | GFAP-Cre:HIF-1αfl/fl | 24 | 11.40 (11.0–12.0) | 0.99 s | 2.81 (2.43–3.17) | 0.99 t | 62.5 | 0.78 u |
HIF-1αfl/fl | 27 | 11.29 (11.0–12.0) | 2.96 (2.64–3.26) | 55.6 | ||||
4 | GFAP-Cre:lysM-Cre:HIF-1αfl/fl | 16 | 11.44 (10.5–12.0) | 0.65 v | 2.56 (2.26–2.88) | 0.98 w | 12.5 | 0.19 x |
HIF-1αfl/fl | 13 | 11.98 (11.5–12.5) | 2.57 (2.27–2.86) | 38.5 |
Experiment 1: EAE induction in lysM-Cre:HIF-1αfl/fl versus control mice HIF-1αfl/fl. Experiment 2: EAE induction in lysMCre:VHLfl/fl versus control mice VHLfl/fl . Experiment 3: EAE induction in GFAP-Cre:HIF-1αfl/fl versus control mice HIF-1αfl/fl . Experiment 4: EAE induction in GFAP-Cre:lysM-Cre:HIF-1αfl/fl versus control mice HIF-1αfl/fl . Day of onset defined as the first day that the score is ≥0.5. A linear mixed-effects model was used to estimate means and 95% confidence interval (CI) for both day of onset and maximum clinical score for each genotype group. A Fisher’s exact test was used to determine whether there is a significant relationship between genotype and mice that achieve score 3 or higher during the experiment (percentage paralysis). Superscript letters refer to the statistical results in Figures 3A, 3B, 5A and 5B. Results, and Table 2.