Table 1.

Materials and reagents

Reagent or resourceSourceCatalog numberRRID
Antibodies
Rabbit anti Iba1Wako019-19471AB_839504
Goat polyclonal anti Iba1Novus BiologicalsNB100-1028AB_521594
Mouse anti-NeuNMilliporeMAB377AB_2298772
Rabbit anti-NeuNCell Signaling24307AB_2651140
Chicken anti-glial fibrillary acidic proteinMilliporeAB5541AB_177521
Rabbit anti-GFPAbcamab290AB_303395
chemicals
Pilocarpine hydrochlorideSigmaP6503N/A
(−)-Scopolamine methyl bromideSigmaS8502N/A
Lectin-DyLight 488Vector LaboratoriesDL-1174AB_2336404
DAPISigmaD9542N/A
Experimental models: organisms/strains
ROSA-Green miceThe Jackson Laboratory007906IMSR_JAX:007906
mT/mG miceThe Jackson Laboratory007676IMSR_JAX:007676
tdTomato miceThe Jackson Laboratory007914IMSR_JAX:007914
Cx3cr1-CreERT2 miceThe Jackson Laboratory020940IMSR_JAX:020940
Cx3cr1-Cre miceMMRRC036395-UCDMMRRC_036395-UCD
Software and algorithms
Fiji-ImageJFiji https://fiji.sc/ or
https://imagej.nih.gov/ij/
SCR_002285
GraphPad Prism 7.0GraphPad Software https://www.graphpad.comSCR_002798
Zen BlackZeiss https://www.zeiss.comN/A
Zen BlueZeiss https://www.zeiss.comSCR_013672
NeurolucidaMBF Bioscience http://www.mbfbioscience.comSCR_001775
  • The source, category, and PRID number of antibodies, chemicals, and mouse lines were provided.